{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T13:34:15Z","timestamp":1788356055411,"version":"build-2803163510"},"reference-count":34,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MAHE","award":["MCODS\/1026\/2023"],"award-info":[{"award-number":["MCODS\/1026\/2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives:Titanium implant surface modifications enhance osseointegration and prevent microbial colonization, improving implant longevity. Antimicrobial coatings, particularly cerium- and bismuth-doped hydroxyapatite (CeHAp and BiHAp), have gained attention for reducing infection-related complications. This study evaluates the antimicrobial activity of CeHAp and BiHAp coatings on CpTi compared to untreated CpTi in artificial saliva at pH levels of 4.5, 6.5, and 8. Methods: Antibacterial efficacy against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Candida albicans (C. albicans) was assessed using the broth dilution method. Titanium rods coated with test compounds were incubated in inoculated nutrient broth, and microbial inhibition was determined via optical density at 600 nm. A statistical analysis was performed using the Kruskal\u2013Wallis ANOVA test, the median and Interquartile Range were determined for the variables, and a Dwass\u2013Steel\u2013Critchlow\u2013Fligner intergroup pairwise comparison was conducted. Results: The results showed that both the CeHAp and BiHAp coatings demonstrated significant antimicrobial activity against S. aureus (OD = 0.01) at pH 6.5, which was more pronounced than the activity observed against E. coli (OD = 0.05), with the difference being statistically significant (p = 0.001). The least antimicrobial activity was observed against C. albicans (0.21) at pH 8 (p = 0.001). Conclusion: These findings highlight the pH-dependent effectiveness of BiHAp and CeHAp coatings in inhibiting microbial growth. Their application on titanium implants may enhance antimicrobial properties, contributing to improved dental implant success and broader biomedical applications.<\/jats:p>","DOI":"10.3390\/antibiotics14070715","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T15:48:22Z","timestamp":1752680902000},"page":"715","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antimicrobial Behavior of Surface-Treated Commercially Pure Titanium (CpTi) for Dental Implants in Artificial Saliva\u2014In Vitro Study"],"prefix":"10.3390","volume":"14","author":[{"given":"Roshni","family":"Bopanna","sequence":"first","affiliation":[{"name":"Department of Periodontology, Manipal College of Dental Science Mangalore, Manipal Academy of Higher Education, Manipal 576 104, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8841-6235","authenticated-orcid":false,"given":"Neetha J.","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Manipal College of Dental Science Mangalore, Manipal Academy of Higher Education, Manipal 576 104, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashith M.","family":"Varadaraj","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopaedics, Manipal College of Dental Science Mangalore, Manipal Academy of Higher Education, Manipal 576 104, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1252-507X","authenticated-orcid":false,"given":"Himani","family":"Kotian","sequence":"additional","affiliation":[{"name":"Lecturer cum Biostatistician, Department of Community Medicine, Kasturba Medical College Mangalore, Manipal Academy of Higher Education, Manipal 576 104, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameep","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Manipal College of Dental Science Mangalore, Manipal Academy of Higher Education, Manipal 576 104, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1614-1256","authenticated-orcid":false,"given":"Simran","family":"Genescia","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Manipal College of Dental Science Mangalore, Manipal Academy of Higher Education, Manipal 576 104, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1111\/jre.12469","article-title":"Synergistic interactions between corrosion and wear at titanium-based dental implant connections: A scoping review","volume":"52","author":"Tarce","year":"2017","journal-title":"J. Periodontal Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Sui, J., Liu, S., Chen, M., and Zhang, H. (2022). Surface Bio-Functionalization of Anti-Bacterial Titanium Implants: A Review. Coatings, 12.","DOI":"10.3390\/coatings12081125"},{"key":"ref_3","first-page":"616","article-title":"Biofilm on dental implants: A review of the literature","volume":"24","author":"Subramani","year":"2009","journal-title":"Int. J. Oral. Maxillofac. Implant."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1177\/0885328211414942","article-title":"In vitro antibacterial activity and cytocompatibility of bismuth doped micro-arc oxidized titanium","volume":"27","author":"Lin","year":"2013","journal-title":"J. Biomater. Appl."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"9519","DOI":"10.1016\/j.biomaterials.2010.08.035","article-title":"Antimicrobial peptides on calcium phosphate-coated titanium for infection prevention","volume":"31","author":"Kindrachuk","year":"2010","journal-title":"Biomaterials"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1126\/science.3629258","article-title":"Biomaterial-centered infection: Microbial adhesion versus tissue integration","volume":"237","author":"Gristina","year":"1987","journal-title":"Science"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1902\/jop.2013.134001","article-title":"Academy Report: Peri-Implant Mucositis and Peri-Implantitis: A Current Understanding of Their Diagnoses and Clinical Implications","volume":"84","author":"Rosen","year":"2013","journal-title":"J. Periodontol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/prd.12185","article-title":"Modern implant dentistry based on osseointegration: 50 years of progress, current trends and open questions","volume":"73","author":"Buser","year":"2017","journal-title":"Periodontol. 2000"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3389\/fmats.2020.00213","article-title":"Cerium and Its Oxidant-Based Nanomaterials for Antibacterial Applications: A State-of-the-Art Review","volume":"7","author":"Qi","year":"2020","journal-title":"Front. Mater."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1302\/2046-3758.85.BJR-2018-0316","article-title":"Antibacterial coating of implants: Are we missing something?","volume":"8","author":"Tsuchiya","year":"2019","journal-title":"Bone Jt. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1021\/acs.bioconjchem.1c00129","article-title":"Overview of Antibacterial Strategies of Dental Implant Materials for the Prevention of Peri-Implantitis","volume":"32","author":"Chen","year":"2021","journal-title":"Bioconjug Chem."},{"key":"ref_12","first-page":"101933","article-title":"Targeting implant-associated infections: Titanium surface loaded with antimicrobial","volume":"24","author":"Souza","year":"2020","journal-title":"iScience"},{"key":"ref_13","first-page":"4292976","article-title":"In vitro evaluation of bacterial adhesion and bacterial viability of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis on the abutment surface of titanium and zirconium dental implants","volume":"2019","author":"Mazulis","year":"2019","journal-title":"Int. J. Dent."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Drayton, M., Kizhakkedathu, J.N., and Straus, S.K. (2020). Towards robust delivery of antimicrobial peptides to combat bacterial resistance. Molecules, 25.","DOI":"10.3390\/molecules25133048"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"10562","DOI":"10.1021\/acsomega.0c00986","article-title":"Poly-l-lysine\/Sodium Alginate Coating Loading Nanosilver for Improving the Antibacterial Effect and Inducing Mineralization of Dental Implants","volume":"5","author":"Guo","year":"2020","journal-title":"ACS Omega"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2218","DOI":"10.1002\/jbm.a.36081","article-title":"Antibacterial and bioactive coatings on titanium implant surfaces","volume":"105","author":"Aranya","year":"2017","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/jre.12737","article-title":"A peptide coating preventing the attachment of Porphyromonas gingivalis on the surfaces of dental implants","volume":"55","author":"Fang","year":"2020","journal-title":"J. Periodontal Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"8090","DOI":"10.1039\/C8TB01918E","article-title":"Surface engineering of titanium implants with enzyme-triggered antibacterial properties and enhanced osseointegration in vivo","volume":"6","author":"Yuan","year":"2018","journal-title":"J. Mater. Chem. B"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.mser.2004.11.001","article-title":"Surface modification of titanium, titanium alloys, and related materials for biomedical applications","volume":"47","author":"Liu","year":"2004","journal-title":"Mater. Sci. Eng. R. Rep."},{"key":"ref_20","first-page":"621","article-title":"Titanium coating: Introducing an antibacterial and bioactive chitosan-alginate film on titanium by spin coating","volume":"65","author":"Vakili","year":"2020","journal-title":"Biomed. Tech."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"124621","DOI":"10.1016\/j.cej.2020.124621","article-title":"Surface modification of titanium implants by ZIF-8@Levo\/LBL coating for inhibition of bacterial-associated infection and enhancement of in vivo osseointegration","volume":"390","author":"Tao","year":"2020","journal-title":"Chem. Eng. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"112041","DOI":"10.1016\/j.msec.2021.112041","article-title":"Multi-functional cerium oxide nanoparticles regulate inflammation and enhance osteogenesis","volume":"124","author":"Wei","year":"2021","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Predoi, D., Iconaru, S.L., Predoi, M.V., Groza, A., Gaiaschi, S., Rokosz, K., Raaen, S., Negrila, C.C., Prodan, A.-M., and Costescu, A. (2020). Development of Cerium-Doped Hydroxyapatite Coatings with Antimicrobial Properties for Biomedical Applications. Coatings, 10.","DOI":"10.3390\/coatings10060516"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1016\/j.jmrt.2021.03.009","article-title":"Structural, morphological and biological evaluations of cerium incorporated hydroxyapatite sol-gel coatings on Ti-6Al-4V for orthopaedic applications","volume":"12","author":"Priyadarshini","year":"2021","journal-title":"J. Mater. Res. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Pop, O.L., Mesaros, A., Vodnar, D.C., Suharoschi, R., T\u0103b\u0103ran, F., Mageru\u0219an, L., T\u00f3dor, I.S., Diaconeasa, Z., Balint, A., and Ciontea, L. (2020). Cerium Oxide Nanoparticles and Their Efficient Antibacterial Application In Vitro against Gram-Positive and Gram-Negative Pathogens. Nanomaterials, 10.","DOI":"10.3390\/nano10081614"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2852","DOI":"10.1016\/j.ceramint.2018.07.290","article-title":"Cerium-doped hydroxyapatite\/collagen coatings on titanium for bone implants","volume":"45","author":"Ciobanu","year":"2019","journal-title":"Ceram. Int."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1002\/jbm.a.37289","article-title":"Investigation on the antimicrobial properties of cerium-doped bioactive glasses","volume":"110","author":"Raimondi","year":"2022","journal-title":"J. Biomed. Mater. Res. A."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2354","DOI":"10.1016\/j.ccr.2007.03.003","article-title":"Biocoordination chemistry of bismuth: Recent advances","volume":"251","author":"Yang","year":"2007","journal-title":"Coord. Chem. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1016\/j.jnoncrysol.2010.11.086","article-title":"Discussion on the origin of NIR emission from Bi-doped materials","volume":"357","author":"Peng","year":"2011","journal-title":"J. Non-Cryst. Solids."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3199","DOI":"10.1016\/j.actbio.2010.02.049","article-title":"Bismuth-doped injectable calcium phosphate cement with improved radiopacity and potent antimicrobial activity for root canal filling","volume":"6","author":"Chen","year":"2010","journal-title":"Acta Biomater."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ciobanu, G., and Harja, M. (2019). Bismuth-doped nanohydroxyapatite coatings on titanium implants for improved radiopacity and antimicrobial activity. Nanomaterials, 9.","DOI":"10.3390\/nano9121696"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"45859","DOI":"10.1038\/srep45859","article-title":"pH-Controlled Cerium Oxide Nanoparticle Inhibition of Both Gram-Positive and Gram-Negative Bacteria Growth","volume":"7","author":"Alpaslan","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3195","DOI":"10.1039\/C6TB00396F","article-title":"Controlling the surface chemistry of cerium oxide nanoparticles for biological applications","volume":"4","author":"Gupta","year":"2016","journal-title":"J. Mater. Chem. B"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1177\/00220345630420051301","article-title":"Corrosion of Gold and Amalgam Placed in Contact with Each Other","volume":"42","author":"Fusayama","year":"1963","journal-title":"J. Dent. Res."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/7\/715\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:10:52Z","timestamp":1760033452000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/7\/715"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,16]]},"references-count":34,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["antibiotics14070715"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics14070715","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,16]]}}}