{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T15:28:48Z","timestamp":1781969328896,"version":"3.54.5"},"reference-count":61,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Pisa, \u201cFondi Ateneo 2023\u201d"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: European hedgehogs (Erinaceus europaeus) are present in areas where there is human activity; therefore, they can be a source of pathogens for other animals and humans. Methods: Eighteen hedgehog carcasses were collected and analyzed for Staphylococcus spp. Isolated strains were typed and analyzed for exfoliative toxins genes and the phenotypic and genotypic characteristics of antimicrobial resistance. Results: A total of 54 strains were isolated and typed as S. aureus, S. xylosus, S. sciuri, S. pseudintermedius, S. simulans, S. chromogenes, S. epidermidis, S. hyicus, and S. lentus. No strains had the eta and etb genes coding for exfoliative toxins. Overall, 39\/54 (72.20%) isolates showed phenotypic resistance to at least one antimicrobial and 21\/54 (38.80%) showed more than one resistance. The lowest efficacy was observed for erythromycin, with 40\/54 (74.08%) strains classified as intermediate and 6\/54 (11.11%) classified as resistant. Among the 29 isolates shown to be penicillin-resistant, 11 (37.93%) were oxacillin-resistant, with a minimum inhibitory concentration (MIC). Among the 54 staphylococcal strains, 2 (3.70%) were resistant to vancomycin, both with an MIC value equal to the maximum concentration of the antibiotic tested (256 \u03bcg\/mL) and 2 (3.70%) had an intermediate resistance profile with an 8 \u03bcg\/mL MIC value. No strains had the genes vanA and vanB. Two of the 29 (6.90%) penicillin-resistant strains had the blaZ gene; 8 (27.13%) strains had the mecA gene. Overall, 2\/54 (3.70%) isolates were classified as extensively drug-resistant (XDR) and 9\/54 (16.66%) were classified as multidrug-resistant (MDR). Conclusions: Hedgehogs can harbor antimicrobial-resistant staphylococci and can be sources of these bacteria for other animals and humans. They can also serve as bioindicators of the pathogens and antimicrobial-resistant bacteria circulating in a given habitat.<\/jats:p>","DOI":"10.3390\/antibiotics14070725","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T14:38:36Z","timestamp":1752849516000},"page":"725","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antimicrobial-Resistant Staphylococcus spp. Harbored by Hedgehogs (Erinaceus europaeus) in Central Italy"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5292-0613","authenticated-orcid":false,"given":"Fabrizio","family":"Bertelloni","sequence":"first","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Pauselli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8198-5865","authenticated-orcid":false,"given":"Giulia","family":"Cagnoli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Biscontri","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2506-6543","authenticated-orcid":false,"given":"Renato","family":"Ceccherelli","sequence":"additional","affiliation":[{"name":"CRUMA-LIPU, Via delle Sorgenti 430, 57121 Livorno, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7997-776X","authenticated-orcid":false,"given":"Valentina Virginia","family":"Ebani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"},{"name":"Centre for Climate Change Impact, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Arnold, K.E., Williams, N.J., and Bennett, M. 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