{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T12:31:04Z","timestamp":1781613064916,"version":"3.54.5"},"reference-count":50,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T00:00:00Z","timestamp":1754265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health","award":["RRC202523685965"],"award-info":[{"award-number":["RRC202523685965"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Enterococci, particularly Enterococcus faecalis and Enterococcus faecium, are Gram-positive cocci that can cause severe infections in hospitalized patients. The rise of vancomycin-resistant enterococci (VRE) and vancomycin-variable enterococci (VVE) poses significant challenges in healthcare settings due to their resistance to multiple antibiotics. Methods: We conducted a point prevalence survey (PPS) to assess the prevalence of VRE and VVE colonization in hospitalized patients. Rectal swabs were collected from 160 patients and analyzed using molecular assays (MAs) and culture. Whole-genome sequencing (WGS) and core-genome multilocus sequence typing (cgMLST) were performed to identify the genetic diversity. Results: Of the 160 rectal swabs collected, 54 (33.7%) tested positive for the vanA and\/or vanB genes. Culture-based methods identified 47 positive samples (29.3%); of these, 44 isolates were identified as E. faecium and 3 as E. faecalis. Based on the resistance profiles, 35 isolates (74.5%) were classified as VRE, while 12 (25.5%) were classified as VVE. WGS and cgMLST analyses identified seven clusters of E. faecium, with sequence type (ST) 80 being the most prevalent. Various resistance genes and virulence factors were identified, and this study also highlighted intra- and inter-ward transmission of VRE strains. Conclusions: Our findings underscore the potential for virulence and resistance of both the VRE and VVE strains, and they highlight the importance of effective infection control measures to prevent their spread. VVE in particular should be carefully monitored as they often escape detection. Integrating molecular data with clinical information will hopefully enhance our ability to predict and prevent future VRE infections.<\/jats:p>","DOI":"10.3390\/antibiotics14080795","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T10:13:51Z","timestamp":1754475231000},"page":"795","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Surveillance and Characterization of Vancomycin-Resistant and Vancomycin-Variable Enterococci in a Hospital Setting"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4214-2812","authenticated-orcid":false,"given":"Claudia","family":"Rotondo","sequence":"first","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9796-7813","authenticated-orcid":false,"given":"Valentina","family":"Antonelli","sequence":"additional","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0940-7583","authenticated-orcid":false,"given":"Alberto","family":"Rossi","sequence":"additional","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7783-6194","authenticated-orcid":false,"given":"Silvia","family":"D\u2019Arezzo","sequence":"additional","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9131-7502","authenticated-orcid":false,"given":"Marina","family":"Selleri","sequence":"additional","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1768-6017","authenticated-orcid":false,"given":"Michele","family":"Properzi","sequence":"additional","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9041-6461","authenticated-orcid":false,"given":"Silvia","family":"Turco","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, 01100 Viterbo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3901-6926","authenticated-orcid":false,"given":"Giovanni","family":"Chillemi","sequence":"additional","affiliation":[{"name":"Bioinformatics Research Unit in Infectious Diseases, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"},{"name":"Department of Experimental Medicine, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4729-577X","authenticated-orcid":false,"given":"Valentina","family":"Dimartino","sequence":"additional","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2644-1862","authenticated-orcid":false,"given":"Carolina","family":"Venditti","sequence":"additional","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Guerci","sequence":"additional","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Gall\u00ec","sequence":"additional","affiliation":[{"name":"Clinical Risk Management, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5369-0437","authenticated-orcid":false,"given":"Carla","family":"Nisii","sequence":"additional","affiliation":[{"name":"Microbiology and Biobank Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Arcangeli","sequence":"additional","affiliation":[{"name":"Clinical Risk Management, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8271-6920","authenticated-orcid":false,"given":"Emanuela","family":"Caraffa","sequence":"additional","affiliation":[{"name":"Systemic and Immune Depression-Associated Infections Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0754-6917","authenticated-orcid":false,"given":"Stefania","family":"Cicalini","sequence":"additional","affiliation":[{"name":"Systemic and Immune Depression-Associated Infections Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2198-1947","authenticated-orcid":false,"given":"Carla","family":"Fontana","sequence":"additional","affiliation":[{"name":"Microbiology 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