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Carlos III (Plan Estatal de I + D+i 2017\u20132020)","award":["PID2021\u2013125597NB-I00"],"award-info":[{"award-number":["PID2021\u2013125597NB-I00"]}]},{"name":"Instituto de Salud Carlos III (Plan Estatal de I + D+i 2017\u20132020)","award":["CP19\/00104"],"award-info":[{"award-number":["CP19\/00104"]}]},{"name":"Instituto de Salud Carlos III (Plan Estatal de I + D+i 2017\u20132020)","award":["TED2021-130793B-I00"],"award-info":[{"award-number":["TED2021-130793B-I00"]}]},{"name":"European Social Fund \u201cInvesting in your future\u201d","award":["CB21\/13\/00043"],"award-info":[{"award-number":["CB21\/13\/00043"]}]},{"name":"European Social Fund \u201cInvesting in your future\u201d","award":["RF20230503223"],"award-info":[{"award-number":["RF20230503223"]}]},{"name":"European Social Fund \u201cInvesting in your future\u201d","award":["PID2021\u2013125597NB-I00"],"award-info":[{"award-number":["PID2021\u2013125597NB-I00"]}]},{"name":"European Social Fund \u201cInvesting in your future\u201d","award":["CP19\/00104"],"award-info":[{"award-number":["CP19\/00104"]}]},{"name":"European Social Fund \u201cInvesting in your future\u201d","award":["TED2021-130793B-I00"],"award-info":[{"award-number":["TED2021-130793B-I00"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n (Proyectos de Transici\u00f3n Ecol\u00f3gica y Digital)","award":["CB21\/13\/00043"],"award-info":[{"award-number":["CB21\/13\/00043"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n (Proyectos de Transici\u00f3n Ecol\u00f3gica y Digital)","award":["RF20230503223"],"award-info":[{"award-number":["RF20230503223"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n (Proyectos de Transici\u00f3n Ecol\u00f3gica y Digital)","award":["PID2021\u2013125597NB-I00"],"award-info":[{"award-number":["PID2021\u2013125597NB-I00"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n (Proyectos de Transici\u00f3n Ecol\u00f3gica y Digital)","award":["CP19\/00104"],"award-info":[{"award-number":["CP19\/00104"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n (Proyectos de Transici\u00f3n Ecol\u00f3gica y Digital)","award":["TED2021-130793B-I00"],"award-info":[{"award-number":["TED2021-130793B-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Mycobacterium abscessus is an opportunistic pathogen causing infections mainly in patients with immunosuppression and chronic pulmonary pathologies. Extended treatment periods are needed to tackle this pathogen, bacterial eradication is rare, and recurrence can take place with time. New alternative treatments are being investigated, such as bacteriophage therapy. This work describes the characterization of the mycobacteriophage P3MA, showing its ability to infect clinical and standard M. abscessus strains. Methods: Phylogenetic analysis, electron microscopy, growth curves, biofilm assays, checkerboard, and granuloma-like medium studies were performed. Results: P3MA inhibited the growth of clinical samples in both planktonic and biofilm states as well as in a granuloma-like model. The study of the interaction with antibiotics revealed that P3MA exhibited an antagonistic effect combined with clarithromycin, indifference with amikacin, and synergy with imipenem. Conclusions: All these results suggest that, after genetic engineering, P3MA could be a promising candidate for phage therapy in combination with imipenem, including lung infections.<\/jats:p>","DOI":"10.3390\/antibiotics14080801","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T10:13:51Z","timestamp":1754475231000},"page":"801","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus"],"prefix":"10.3390","volume":"14","author":[{"given":"Antonio","family":"Broncano-Lavado","sequence":"first","affiliation":[{"name":"Clinical Microbiology Department, IIS-Fundaci\u00f3n Jim\u00e9nez D\u00edaz, Autonomous University of Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Jairo","family":"Aguilera-Correa","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier, Centre National de la Recherche Scientifique UMR 9004, Universit\u00e9 de Montpellier, 34293 Montpellier, France"},{"name":"CIBERINFEC-Consorcio Centro de Investigaci\u00f3n Biom\u00e9dica en Red (CIBER) de Enfermedades Infecciosas, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2636-3734","authenticated-orcid":false,"given":"Fran\u00e7oise","family":"Roquet-Ban\u00e8res","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier, Centre National de la Recherche Scientifique UMR 9004, Universit\u00e9 de Montpellier, 34293 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6604-4458","authenticated-orcid":false,"given":"Laurent","family":"Kremer","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier, Centre National de la Recherche Scientifique UMR 9004, Universit\u00e9 de Montpellier, 34293 Montpellier, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Institut de Recherche en Infectiologie de Montpellier, 34293 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5368-574X","authenticated-orcid":false,"given":"Ar\u00e1nzazu","family":"Mediero","sequence":"additional","affiliation":[{"name":"Bone and Joint Research Unit, IIS-Fundaci\u00f3n Jim\u00e9nez D\u00edaz, Autonomous University of Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4704-3131","authenticated-orcid":false,"given":"Mateo","family":"Seoane-Blanco","sequence":"additional","affiliation":[{"name":"Department of Macromolecular Structure, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cient\u00edficas, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4781-1375","authenticated-orcid":false,"given":"Mark J.","family":"van Raaij","sequence":"additional","affiliation":[{"name":"Department of Macromolecular Structure, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cient\u00edficas, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8876-1194","authenticated-orcid":false,"given":"Israel","family":"Pag\u00e1n","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas UPM-INIA\/CSIC and E.T.S. 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