{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T14:29:23Z","timestamp":1793284163641,"version":"4.5.0"},"reference-count":73,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,10]],"date-time":"2025-08-10T00:00:00Z","timestamp":1754784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Introduction:\u00a0Staphylococcus aureus is a high-priority foodborne pathogen contributing to several food poisoning outbreaks. Methicillin- and vancomycin-resistant S. aureus (MRSA and VRSA), pose significant public health concerns due to their potential for serious illness, antibiotic resistance, and transmission within both healthcare and community settings. These bacteria can cause numerous infections, ranging from skin and soft tissue infections to life-threatening conditions like bloodstream infections, pneumonia, and endocarditis. Although several publications are concerned with Staphylococcus aureus contamination in ready-to-eat (RTE) food products, little published data is available about its prevalence in pizza, which is widely distributed and consumed worldwide. Methods: The current study is intended to determine the prevalence, virulence genes, and antimicrobial resistance profiles of S. aureus in three hundred ready-to-eat pizza samples (100 each of meat, chicken, and canned tuna pizzas) collected from different restaurants in Mansoura City, Egypt. The typical colonies on Baird\u2013Parker selective agar supplemented with egg yolk tellurite emulsion were counted and further confirmed based on Gram staining, coagulase testing, catalase testing, carbohydrate fermentation, and thermostable nuclease production. The genomic DNA of the confirmed coagulase-positive isolates was prepared and subjected to PCR analyses for detecting the nuc gene, mecA (methicillin resistance gene), and vancomycin resistance gene (vanA), as well as six selected S. aureus virulence genes: sea, seb, sec, sed, hla, and tsst. The antimicrobial resistance profile of the S. aureus isolates was determined against 16 antimicrobial agents belonging to six classes using the agar disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines (CLSI), except for oxacillin and vancomycin, which were assessed using the MIC test. Results: The results revealed that 56% (56\/100), 56% (56\/100), and 40% (40\/100) of chicken, meat, and canned tuna pizzas were positive for S. aureus, with an overall prevalence of 50.7% (152\/300). All 560 isolates (100%) were verified as S. aureus based on molecular confirmation of the nuc gene. Interestingly, 48.6% (272\/560) and 8.6% (48\/560) of the isolates tested were identified as methicillin- and vancomycin-resistant S. aureus (MRSA and VRSA) through detection of mecA and vanA genes, respectively. Among the S. aureus isolates tested, the hla gene was detected in 87.1% (488\/560), while the enterotoxin genes sea, seb, sec, and sed were identified in 50% (280\/560), 78.6% (440\/560), 9.8% (55\/560), and 24.5% (137\/560) of isolates, respectively. All recovered isolates (n = 560) were classified as multidrug-resistant and were resistant to penicillin, oxacillin, and ampicillin. Moreover, 77% (431\/560), 24% (134\/560), 8% (45\/560), and 8.6% (48\/560) of isolates were resistant to cefotaxime, ciprofloxacin, azithromycin, and vancomycin, respectively. Conclusions: The current study emphasizes that ready-to-eat pizza is highly contaminated with multidrug-resistant S. aureus, highlighting the urgent need for rationalizing antibiotic use in both veterinary and human medicine to prevent the transmission of resistant bacteria through the food chain. Additionally, strict adherence to good hygienic practices throughout all stages of the food chain is essential to minimize overall contamination and enhance food safety.<\/jats:p>","DOI":"10.3390\/antibiotics14080817","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T11:02:09Z","timestamp":1754910129000},"page":"817","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Prevalence and Characterization of the Antimicrobial Resistance and Virulence Profiles of Staphylococcus aureus in Ready-to-Eat (Meat, Chicken, and Tuna) Pizzas in Mansoura City, Egypt"],"prefix":"10.3390","volume":"14","author":[{"given":"Sara Amgad","family":"Elsalkh","sequence":"first","affiliation":[{"name":"Department of Food Hygiene, Safety, and Technology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira Ibrahim","family":"Zakaria","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene, Safety, and Technology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir Mohammed","family":"Abd-Elghany","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene, Safety, and Technology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6057-882X","authenticated-orcid":false,"given":"K\u00e1lm\u00e1n","family":"Imre","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4701-4229","authenticated-orcid":false,"given":"Adriana","family":"Morar","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3435-9436","authenticated-orcid":false,"given":"Khalid Ibrahim","family":"Sallam","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene, Safety, and Technology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,10]]},"reference":[{"key":"ref_1","unstructured":"Dodd, C.E.R., Aldsworth, T.G., Stein, R.A., and Cliver, D.O. (2003). Staphylococcus\u00a0aureus. International Handbook of Foodborne Pathogens, CRC Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2933","DOI":"10.3168\/jds.2010-3956","article-title":"Distribution of coagulase-negative Staphylococcus species from milk and environment of dairy cows differs between herds","volume":"94","author":"Piessens","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Puah, S.M., Chua, K.H., and Tan, J.A.M.A. (2016). Virulence factors and antibiotic susceptibility of Staphylococcus aureus isolates in ready-to-eat foods: Detection of S. aureus contamination and a high prevalence of virulence genes. Int. J. Environ. Res. Public Health, 13.","DOI":"10.3390\/ijerph13020199"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Bhunia, A.K. (2018). Staphylococcus\u00a0aureus. Foodborne Microbial Pathogens, Springer.","DOI":"10.1007\/978-1-4939-7349-1"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3201\/eid1701.P11101","article-title":"Foodborne illness acquired in the United States\u2014Major pathogens","volume":"17","author":"Scallan","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kadariya, J., Smith, T.C., and Thapaliya, D. (2014). Staphylococcus aureus and staphylococcal food-borne disease: An ongoing challenge in public health. BioMed Res. Int., 2014.","DOI":"10.1155\/2014\/827965"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.mib.2013.11.004","article-title":"Staphylococcus aureus toxins","volume":"17","author":"Otto","year":"2014","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1111\/j.1574-6976.2011.00311.x","article-title":"Staphylococcus aureus and its food poisoning toxins: Characterization and outbreak investigation","volume":"36","author":"Hennekinne","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0168-1605(01)00443-3","article-title":"Implication of milk and milk products in food-borne diseases in France and in different industrialised countries","volume":"67","author":"Dufour","year":"2001","journal-title":"Int. J. Food Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Morar, A., Ban-Cucerzan, A., Herman, V., T\u00eerziu, E., Sallam, K.I., Abd-Elghany, S.M., and Imre, K. (2021). Multidrug Resistant Coagulase-Positive Staphylococcus aureus and Their Enterotoxins Detection in Traditional Cheeses Marketed in Banat Region, Romania. Antibiotics, 10.","DOI":"10.3390\/antibiotics10121458"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/microbiolspec.GPP3-0057-2018","article-title":"Antibiotic resistance and the MRSA problem","volume":"7","author":"Vestergaard","year":"2019","journal-title":"Microbiol. Spectr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.tim.2013.11.003","article-title":"The emergence of mecC methicillin-resistant Staphylococcus aureus","volume":"22","author":"Paterson","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_13","unstructured":"NFSA (Egypt\u2019s National Food Safety Authority) (2021). Egypt\u2019s National Food Safety Authority Issues New Decision Regulating Microbiological Contaminants, Official Gazette. Decision of the Board of Directors No. (1) of the Year 2021 on the Technical Regulations of Microbiological Criteria for Food."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"12","DOI":"10.21608\/bvmj.2019.15641.1063","article-title":"Prevalence of methicillin-resistant Staphylococcus aureus in some ready-to-eat meat products","volume":"37","author":"Saifm","year":"2019","journal-title":"Benha Vet. Med. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1501\/Vetfak_0000002628","article-title":"Microbiological evaluation of ready-to-eat sandwiches served near hospitals and schools","volume":"61","author":"Beyaz","year":"2014","journal-title":"Ankara \u00dcniv. Vet. Fak. Derg."},{"key":"ref_16","first-page":"595","article-title":"Microbial quality of cooked meat foods in Tehran University\u2019s restaurants","volume":"24","author":"Tavakoli","year":"2008","journal-title":"Pak. J. Med. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1111\/j.1745-4565.2010.00221.x","article-title":"Detection and enumeration of microorganisms in ready-to-eat foods, ready-to-cook foods, and fresh-cut produce in Korea","volume":"30","author":"Chung","year":"2010","journal-title":"J. Food Saf."},{"key":"ref_18","first-page":"1324","article-title":"Prevalence of methicillin-resistant Staphylococcus aureus in raw hamburgers and ready-to-eat sandwiches commercialized in supermarkets and fast food outlets in Brazil","volume":"6","author":"Contreras","year":"2015","journal-title":"Food Nutr. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wu, S., Huang, J., Wu, Q., Zhang, J., Zhang, F., Yang, X., Wu, H., Zen, H., Chen, M., and Ding, Y. (2018). Staphylococcus aureus isolated from retail meat and meat products in China: Incidence, antibiotic resistance and genetic diversity. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.02767"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Mahros, M.A., Abd-Elghany, S.M., and Sallam, K.I. (2021). Multidrug-, methicillin-, and vancomycin-resistant Staphylococcus aureus isolated from ready-to-eat meat sandwiches: An ongoing food and public health concern. Int. J. Food Microbiol., 346.","DOI":"10.1016\/j.ijfoodmicro.2021.109165"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"110117","DOI":"10.1016\/j.foodcont.2023.110117","article-title":"Exploring the potential of zinc oxide nanoparticles against pathogenic multi-drug resistant Staphylococcus aureus from ready-to-eat meat and its proposed mechanism","volume":"156","author":"Sobhy","year":"2024","journal-title":"Food Control"},{"key":"ref_22","first-page":"377","article-title":"Quality assessment of some imported and local canned tuna sold in Kafrelsheikh, Egypt","volume":"13","author":"Nagy","year":"2023","journal-title":"J. Adv. Vet. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.foodcont.2014.01.039","article-title":"Growth of Staphylococcus aureus and enterotoxin production in pre-cooked tuna meat","volume":"42","author":"Wu","year":"2014","journal-title":"Food Control"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"231","DOI":"10.4315\/0362-028X.JFP-12-335","article-title":"Microbiological quality and risk factors related to sandwiches served in bakeries, caf\u00e9s, and sandwich bars in South Korea","volume":"76","author":"Jang","year":"2013","journal-title":"J. Food Prot."},{"key":"ref_25","first-page":"281","article-title":"Antimicrobial susceptibility and molecular typing of methicillin-resistant Staphylococcus aureus in retail foods in Shaanxi, China","volume":"11","author":"Wang","year":"2014","journal-title":"Pathog. Dis."},{"key":"ref_26","first-page":"63","article-title":"Phenotypic and genotypic characterization of tetracycline-resistant Staphylococcus aureus isolated from food of animal origin","volume":"9","author":"Arafa","year":"2017","journal-title":"Egypt. J. Environ. Res."},{"key":"ref_27","first-page":"1479","article-title":"Prevalence of multidrug-resistant Staphylococcus aureus in meals served at hospitals","volume":"56","author":"Ghanem","year":"2025","journal-title":"Egypt. J. Vet. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1893\/0005-3155-83.1.33","article-title":"Prevalence of MRSA on meat products in central Kentucky","volume":"83","author":"Wells","year":"2012","journal-title":"Bios"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s13756-017-0257-1","article-title":"Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food","volume":"6","author":"Gandomi","year":"2017","journal-title":"J. Antimicrob. Resist. Infect. Control"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1093\/jac\/dkr437","article-title":"MRSA: The first half century","volume":"67","author":"Moellering","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1177\/1757913917743881","article-title":"WHO global priority pathogens list on antibiotic resistance: An urgent need for action to integrate One Health data","volume":"138","author":"Asokan","year":"2018","journal-title":"Perspect. Public Health"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.cofs.2019.04.002","article-title":"Klebsiella pneumoniae carbapenemase (KPC), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococcus spp. (VRE) in the food production chain and biofilm formation on abiotic surfaces","volume":"26","author":"Alonso","year":"2019","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Maharjan, M., Sah, A.K., Pyakurel, S., Thapa, S., Maharjan, S., Adhikari, N., Rijal, K.R., Ghimire, P., and Shrestha, U.T. (2021). Molecular confirmation of vancomycin-resistant Staphylococcus aureus with vanA gene from a hospital in Kathmandu. Int. J. Microbiol., 2021.","DOI":"10.1155\/2021\/3847347"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.jare.2019.10.005","article-title":"Vancomycin-resistant Staphylococcus aureus infections: A review of case updating and clinical features","volume":"21","author":"Cong","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Saber, T., Samir, M., El-Mekkawy, R.M., Ariny, E., El-Sayed, S.R., Enan, G., Abdelatif, S.H., Askora, A., Merwad, A.M.A., and Tartor, Y.H. (2022). Methicillin- and vancomycin-resistant Staphylococcus aureus from humans and ready-to-eat meat: Characterization of antimicrobial resistance and biofilm formation ability. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.735494"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Afshari, A., Taheri, S., Hashemi, M., Norouzy, A., Nematy, M., and Mohamadi, S. (2022). Methicillin- and vancomycin-resistant Staphylococcus aureus and vancomycin-resistant enterococci isolated from hospital foods: Prevalence and antimicrobial resistance patterns. Curr. Microbiol., 79.","DOI":"10.1007\/s00284-022-03022-0"},{"key":"ref_37","unstructured":"World Health Organization (2017). WHO Publishes List of Bacteria for Which New Antibiotics Are Urgently Needed, World Health Organization. Available online: https:\/\/www.who.int\/en\/news-room\/detail\/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Ertas Onmaz, N., Demirezen Yilmaz, D., Imre, K., Morar, A., Gungor, C., Yilmaz, S., Gundog, D.A., Dishan, A., Herman, V., and Gungor, G. (2022). Green Synthesis of Gold Nanoflowers Using Rosmarinus officinalis and Helichrysum italicum Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111466"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Chieffi, D., Fanelli, F., Cho, G.S., Schubert, J., Blaiotta, G., Franz, C.M.A.P., Bania, J., and Fusco, V. (2020). Novel insights into the enterotoxigenic potential and genomic background of Staphylococcus aureus isolated from raw milk. J. Food Microbiol., 90.","DOI":"10.1016\/j.fm.2020.103482"},{"key":"ref_40","first-page":"63","article-title":"Staphylococcus aureus and food poisoning","volume":"2","author":"Baron","year":"2003","journal-title":"Genet. Mol. Res."},{"key":"ref_41","first-page":"353","article-title":"Growth limits of Staphylococcus aureus as a function of temperature, acetic acid, NaCl concentration, and inoculum level","volume":"9","author":"Jamshidi","year":"2008","journal-title":"Iran. J. Vet. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.3390\/toxins2071751","article-title":"Food poisoning and Staphylococcus aureus enterotoxins","volume":"2","author":"Mendoza","year":"2010","journal-title":"Toxins"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Wu, S., Huang, J., Zhang, F., Wu, Q., Zhang, J., Pang, R., Zeng, H., Yang, X., Chen, M., and Wang, G. (2019). Prevalence and characterization of food-related methicillin-resistant Staphylococcus aureus (MRSA) in China. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00304"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.4315\/0362-028X.JFP-16-532","article-title":"Prevalence of staphylococcal enterotoxins in ready-to-eat foods sold in Istanbul","volume":"80","author":"Ulusoy","year":"2017","journal-title":"J. Food Prot."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Hughes, A.C., Kirkland, M., Du, W., Rasooly, R., Hernlem, B., Tam, C., Zhang, Y., and He, X. (2023). Development of thermally stable nanobodies for detection and neutralization of staphylococcal enterotoxin B. Toxins, 15.","DOI":"10.3390\/toxins15060400"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.3390\/toxins2082177","article-title":"Staphylococcal enterotoxins","volume":"2","author":"Pinchuk","year":"2010","journal-title":"Toxins"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.4315\/0362-028X.JFP-14-027","article-title":"Virulence and resistance gene profiles of Staphylococcus aureus strains isolated from ready-to-eat foods","volume":"77","author":"Baumgartner","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_48","first-page":"388","article-title":"Staphylococcus aureus contamination during food preparation, processing and handling","volume":"5","author":"Mahmoud","year":"2014","journal-title":"Int. J. Chem. Eng. Appl."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/S0378-1097(02)01193-X","article-title":"The effect of cell density and specific growth rate on accessory gene regulator and toxic shock syndrome toxin-1 gene expression in Staphylococcus aureus","volume":"218","author":"Wright","year":"2003","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1501\/Vetfak_0000002749","article-title":"Isolation and characterization of Staphylococcus aureus strains isolated from beef, sheep and chicken meat","volume":"63","author":"Keyvan","year":"2016","journal-title":"J. Ankara \u00dcniv. Vet. Fak. Derg."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"110042","DOI":"10.1016\/j.lwt.2020.110042","article-title":"Characterization of enterotoxigenic Staphylococcus aureus from ready-to-eat seafood (RTES)","volume":"135","author":"Beshiru","year":"2021","journal-title":"LWT"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/S1473-3099(18)30605-4","article-title":"Attributable deaths and disability-adjusted life-years caused by infections with antibiotic-resistant bacteria in the EU and the European Economic Area in 2015: A population-level modeling analysis","volume":"19","author":"Cassini","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Elshebrawy, H.A., Kasem, N.G., and Sallam, K.I. (2025). Methicillin- and vancomycin-resistant Staphylococcus aureus in chicken carcasses, ready-to-eat chicken meat sandwiches, and buffalo milk. Int. J. Food Microbiol., 427.","DOI":"10.1016\/j.ijfoodmicro.2024.110968"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.4315\/0362-028X.JFP-15-150","article-title":"Molecular characterization and antimicrobial resistance profile of methicillin-resistant Staphylococcus aureus in retail chicken","volume":"78","author":"Sallam","year":"2015","journal-title":"J. Food Prot."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Yang, X., Zhang, J., Yu, S., Wu, Q., Guo, W., Huang, J., and Cai, S. (2016). Prevalence of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in retail ready-to-eat foods in China. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00816"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fm.2016.03.004","article-title":"Prevalence, antimicrobial susceptibility and molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) in bulk tank milk from southern Italy","volume":"58","author":"Parisi","year":"2016","journal-title":"Food Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Childs-Kean, L.M., Shaeer, K.M., Varghese Gupta, S., and Cho, J.C. (2019). Aminoglycoside allergic reactions. Pharmacy, 7.","DOI":"10.3390\/pharmacy7030124"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.ijfoodmicro.2017.03.001","article-title":"Methicillin-resistant and -susceptible Staphylococcus aureus from retail meat in Denmark","volume":"249","author":"Tang","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Sadiq, A., Samad, M., Basharat, N., Ali, S., Saad, Z., Khan, A.N., Ahmed, Y., and Khan, A. (2020). Methicillin-resistant Staphylococcus aureus (MRSA) in slaughterhouses and meat shops in the capital territory of Pakistan during 2018\u20132019. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.577707"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1128\/JCM.03166-12","article-title":"Prevalence and characterization of methicillin-resistant Staphylococcus aureus isolates from retail meat and humans in Georgia","volume":"51","author":"Jackson","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"993","DOI":"10.4315\/0362-028X.JFP-13-466","article-title":"Retail ready-to-eat food as a potential vehicle for Staphylococcus spp. harboring antibiotic resistance genes","volume":"77","author":"Zadernowska","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s41182-021-00366-4","article-title":"A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran","volume":"49","author":"Mesbah","year":"2021","journal-title":"Trop. Med. Health"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Chieffi, D., Fanelli, F., and Fusco, V. (2023). Antimicrobial and biocide resistance in Staphylococcus aureus: Genomic features, decontamination strategies, and the role of S. aureus complex-related species, with a focus on ready-to-eat food and food-contact surfaces. Front. Food Sci. Technol., 3.","DOI":"10.3389\/frfst.2023.1165871"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Lin, Q., Sun, H., Yao, K., Cai, J., Ren, Y., and Chi, Y. (2019). The prevalence, antibiotic resistance and biofilm formation of Staphylococcus aureus in bulk ready-to-eat foods. Biomolecules, 9.","DOI":"10.3390\/biom9100524"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Beshiru, A., Isichei-Ukah, B.O., Uwhuba, K.E., Igere, B.E., and Igbinosa, E.O. (2024). Prevalence, characterization, and implications of methicillin-resistant Staphylococcus aureus (MRSA) in ready-to-eat foods from Delta, Nigeria: A concern for consumer safety. Sustain. Microbiol., 1.","DOI":"10.1093\/sumbio\/qvae007"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Adeyemi, F.M., Oyedara, O.O., Yusuf-Omoloye, N.A., Ajigbewu, O.H., Ndaji, O.L., Adegbite-Badmus, M.K., Olumakinde, T.S., and Oluokun, T.E. (2024). Guardians of resistance and virulence: Detection of mec, femA, Van, pvl, hlg and spa genes in methicillin- and vancomycin-resistant Staphylococcus aureus from clinical and food samples in Southwestern Nigeria. BMC Microbiol., 24.","DOI":"10.1186\/s12866-024-03660-3"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1749-6632.2011.05962.x","article-title":"Linezolid, the first oxazolidinone antibacterial agent","volume":"1222","author":"Leach","year":"2011","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1128\/AAC.00775-08","article-title":"Linezolid alone or combined with rifampin against methicillin-resistant Staphylococcus aureus in experimental foreign-body infection","volume":"53","author":"Baldoni","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1007\/s13765-013-4012-x","article-title":"Characterization of antibiotic-resistant Staphylococcus aureus isolates from ready-to-eat foods in Korea","volume":"57","author":"Park","year":"2014","journal-title":"J. Korean Soc. Appl. Biol. Chem."},{"key":"ref_70","unstructured":"(2021). Microbiology of the Food Chain\u2014Horizontal Method for the Enumeration of Coagulase Positive Staphylococci (Staphylococcus aureus and Other Species)\u2014Part 1: Method Using Baird-Parker Agar Medium (Standard No. ISO 6888-1:2021-08)."},{"key":"ref_71","first-page":"416","article-title":"Study on the hemolysin phenotype and the genotype distribution of Staphylococcus aureus causing bovine mastitis in Shandong dairy farms","volume":"9","author":"Wang","year":"2011","journal-title":"Int. J. Appl. Res. Vet. Med."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1128\/JCM.38.3.1032-1035.2000","article-title":"Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance","volume":"38","author":"Mehrotra","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_73","unstructured":"CLSI (Clinical and Laboratory Standards Institute) (2020). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [30th ed.]. CLSI Supplement M100."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/8\/817\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:27:31Z","timestamp":1760034451000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/8\/817"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,10]]},"references-count":73,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["antibiotics14080817"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics14080817","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,10]]}}}