{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:45:27Z","timestamp":1788885927055,"version":"build-2803163510"},"reference-count":39,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,10]],"date-time":"2025-08-10T00:00:00Z","timestamp":1754784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["2022\/11917-1"],"award-info":[{"award-number":["2022\/11917-1"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["309921\/2023-6"],"award-info":[{"award-number":["309921\/2023-6"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["2022\/11917-1"],"award-info":[{"award-number":["2022\/11917-1"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["309921\/2023-6"],"award-info":[{"award-number":["309921\/2023-6"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["2022\/11917-1"],"award-info":[{"award-number":["2022\/11917-1"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["309921\/2023-6"],"award-info":[{"award-number":["309921\/2023-6"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["001"],"award-info":[{"award-number":["001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Extraintestinal pathogenic Escherichia coli (ExPEC) strains, particularly those belonging to phylogenetic group B2, are clinically significant due to their frequent involvement in urinary tract infections (UTIs) and display antimicrobial resistance profiles. While the association of phylogroup B2 E. coli with human urinary tract infections is well established, the growing number of reports of ExPEC strains in canine UTIs highlights their clinical relevance in small animal medicine and raises concerns about their potential role in zoonotic transmission. This study investigated the microbiological and genomic features of E. coli strains isolated from dogs with UTIs in S\u00e3o Paulo, Brazil. Methods: Between March and May 2023, a total of 60 E. coli strains from canine UTIs were screened for antimicrobial susceptibility and phylotyping. Accordingly, four strains (6.6%) were identified as multidrug-resistant (MDR) or belonging to phylogroup B2 and, therefore, were submitted for characterization by whole-genome sequencing. Results: The four E. coli strains exhibited diverse antimicrobial resistance profiles, including resistance to third- and fourth-generation cephalosporins and fluoroquinolones. Phylogenetic groups B1, B2, and G, and sequence types (ST) 73, ST224, ST1193, and ST12960 were identified. The resistome included clinically important \u03b2-lactam resistance genes, such as blaCTX-M-55 and blaCMY-2, as well as mutations in the quinolone-resistance-determining region. Virulence factors associated with ExPEC pathogenesis, including adhesion, iron acquisition, immune evasion, and toxin, were detected. Plasmid sequences were identified as carrying antimicrobial resistance and virulence genes, highlighting the potential for horizontal gene transfer. Conclusions: Our findings underscore the importance of genomic surveillance in companion animals to better understand the epidemiology of ExPEC strains and monitor the spread of MDR strains.<\/jats:p>","DOI":"10.3390\/antibiotics14080819","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T11:02:09Z","timestamp":1754910129000},"page":"819","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clonal Diversity of Extraintestinal Pathogenic Escherichia coli Strains Isolated from Canine Urinary Tract Infections in Brazil"],"prefix":"10.3390","volume":"14","author":[{"given":"Luciana","family":"Sartori","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Pedro Rueda","family":"Furlan","sequence":"additional","affiliation":[{"name":"Paulista School of Medicine, Federal University of Sao Paulo, S\u00e3o Paulo 04039-032, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4725-0125","authenticated-orcid":false,"given":"F\u00e1bio Parra","family":"Sellera","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Metropolitan University of Santos, Santos 11045-002, Brazil"},{"name":"Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5163-0131","authenticated-orcid":false,"given":"Fernanda Borges","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohanna Carvalho dos Santos Aoun","family":"Chikhani","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Infectious Diseases, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5284-6502","authenticated-orcid":false,"given":"Gabriel","family":"Gandolfi","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7107-5710","authenticated-orcid":false,"given":"Terezinha","family":"Kn\u00f6bl","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Foster-Nyarko, E., and Pallen, M.J. (2022). The microbial ecology of Escherichia coli in the vertebrate gut. FEMS Microbiol. Rev., 46.","DOI":"10.1093\/femsre\/fuac008"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Geurtsen, J., de Been, M., Weerdenburg, E., Zomer, A., McNally, A., and Poolman, J. (2022). Genomics and pathotypes of the many faces of Escherichia coli. FEMS Microbiol. Rev., 46.","DOI":"10.1093\/femsre\/fuac031"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Massella, E., Giacometti, F., Bonilauri, P., Reid, C.J., Djordjevic, S.P., Merialdi, G., Bacci, C., Fiorentini, L., Massi, P., and Bardasi, L. (2021). Antimicrobial resistance profile and ExPEC virulence potential in commensal Escherichia coli of multiple sources. Antibiotics, 10.","DOI":"10.3390\/antibiotics10040351"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Rezatofighi, S.E., Mirzarazi, M., and Salehi, M. (2021). Virulence genes and phylogenetic groups of uropathogenic Escherichia coli isolates from patients with urinary tract infection and uninfected control subjects: A case-control study. BMC Infect. Dis., 21.","DOI":"10.1186\/s12879-021-06036-4"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ram\u00edrez-Castillo, F.Y., Moreno-Flores, A.C., Avelar-Gonz\u00e1lez, F.J., M\u00e1rquez-D\u00edaz, F., Harel, J., and Guerrero-Barrera, A.L. (2018). An evaluation of multidrug-resistant Escherichia coli isolates in urinary tract infections from Aguascalientes, Mexico: Cross-sectional study. Ann. Clin. Microbiol. Antimicrob., 17.","DOI":"10.1186\/s12941-018-0286-5"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Whelan, S., Lucey, B., and Finn, K. (2023). Uropathogenic Escherichia coli (UPEC)-associated urinary tract infections: The molecular basis for challenges to effective treatment. Microorganisms, 11.","DOI":"10.3390\/microorganisms11092169"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"105493","DOI":"10.1016\/j.meegid.2023.105493","article-title":"Characterization of uropathogenic Escherichia coli phylogroups associated with antimicrobial resistance, virulence factor distribution, and virulence-related phenotypes","volume":"114","author":"Wang","year":"2023","journal-title":"Infect. Genet. Evol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kidsley, A.K., O\u2019Dea, M., Saputra, S., Jordan, D., Johnson, J.R., Gordon, D.M., Turni, C., Djordjevic, S.P., Abraham, S., and Trott, D.J. (2020). Genomic analysis of phylogenetic group B2 extraintestinal pathogenic E. coli causing infections in dogs in Australia. Vet. Microbiol., 248.","DOI":"10.1016\/j.vetmic.2020.108783"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Sroithongkham, P., Nittayasut, N., Yindee, J., Nimsamer, P., Payungporn, S., Pinpimai, K., Ponglowhapan, S., and Chanchaithong, P. (2024). Multidrug-resistant Escherichia coli causing canine pyometra and urinary tract infections are genetically related but distinct from those causing prostatic abscesses. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-62028-9"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Cozma, A.P., Rimbu, C.M., Zendri, F., Maciuca, I.E., and Timofte, D. (2022). Clonal dissemination of extended-spectrum cephalosporin-resistant Enterobacterales between dogs and humans in households and animal shelters of Romania. Antibiotics, 11.","DOI":"10.3390\/antibiotics11091242"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1038\/nrg3226","article-title":"Transforming clinical microbiology with bacterial genome sequencing","volume":"13","author":"Didelot","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3107","DOI":"10.1111\/1462-2920.14713","article-title":"Characterization and rapid identification of phylogroup G in Escherichia coli, a lineage with high virulence and antibiotic resistance potential","volume":"21","author":"Clermont","year":"2019","journal-title":"Environ. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e01291-22","DOI":"10.1128\/spectrum.01291-22","article-title":"Genomic and temporal trends in canine ExPEC reflect those of human ExPEC","volume":"10","author":"Elankumaran","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Nittayasut, N., Yindee, J., Boonkham, P., Yata, T., Suanpairintr, N., and Chanchaithong, P. (2021). Multiple and high-risk clones of extended-spectrum cephalosporin-resistant and blaNDM-5-harbouring uropathogenic Escherichia coli from cats and dogs in Thailand. Antibiotics, 10.","DOI":"10.3390\/antibiotics10111374"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kidsley, A.K., White, R.T., Beatson, S.A., Saputra, S., Schembri, M.A., Gordon, D., Johnson, J.R., O\u2019Dea, M., Mollinger, J.L., and Abraham, S. (2020). Companion animals are spillover hosts of the multidrug-resistant human extraintestinal Escherichia coli pandemic clones ST131 and ST1193. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01968"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.tvjl.2019.02.008","article-title":"International Society for Companion Animal Infectious Diseases (ISCAID) guidelines for the diagnosis and management of bacterial urinary tract infections in dogs and cats","volume":"247","author":"Weese","year":"2019","journal-title":"Vet. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1007\/s40265-019-01180-3","article-title":"Extended-spectrum \u03b2-lactamase-producing Enterobacteriaceae: Update on molecular epidemiology and treatment options","volume":"79","author":"Peirano","year":"2019","journal-title":"Drugs"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100236","DOI":"10.1016\/j.onehlt.2021.100236","article-title":"Global prevalence and molecular characterization of extended-spectrum \u03b2-lactamase-producing Escherichia coli in dogs and cats\u2013a scoping review and meta-analysis","volume":"12","author":"Benavides","year":"2021","journal-title":"One Health"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"117074","DOI":"10.1016\/j.envpol.2021.117074","article-title":"Biocide-tolerance and antibiotic-resistance in community environments and risk of direct transfers to humans: Unintended consequences of community-wide surface disinfecting during COVID-19?","volume":"283","author":"Chen","year":"2021","journal-title":"Environ. Pollut."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s13567-025-01493-0","article-title":"Zoonotic potential of uropathogenic Escherichia coli lineages from companion animals","volume":"56","author":"Jousserand","year":"2025","journal-title":"Vet. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1093\/jac\/dkaf130","article-title":"A comparative study of the molecular characteristics of human uropathogenic Escherichia coli collected from two hospitals in Norway and France in 2019","volume":"80","author":"Handal","year":"2025","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"da Silva, M.M., Sellera, F.P., Furlan, J.P.R., Aravena-Ram\u00edrez, V., Fuentes-Castillo, D., Fuga, B., Dos Santos Fr\u00f3es, A.J., de Sousa, A.L., Garino Junior, F., and Lincopan, N. (2025). Gut colonization of semi-aquatic turtles inhabiting the Brazilian Amazon by international clones of CTX-M-8-producing Escherichia coli. Vet. Microbiol., 301.","DOI":"10.1016\/j.vetmic.2024.110344"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.jgar.2018.10.023","article-title":"Genomic features of a highly virulent, ceftiofur-resistant, CTX-M-8-producing Escherichia coli ST224 causing fatal infection in a domestic cat","volume":"15","author":"Silva","year":"2018","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jgar.2025.06.011","article-title":"Occurrence of mcr-1.1-producing Escherichia coli in clinical settings in Tunisia","volume":"44","author":"Jaidane","year":"2025","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Fuga, B., Sellera, F.P., Cerdeira, L., Esposito, F., Cardoso, B., Fontana, H., Moura, Q., Cardenas-Arias, A., Sano, E., and Ribas, R.M. (2022). WHO critical priority Escherichia coli as One Health challenge for a post-pandemic scenario: Genomic surveillance and analysis of current trends in Brazil. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.01256-21"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e02554-22","DOI":"10.1128\/spectrum.02554-22","article-title":"Global phylogeny and F virulence plasmid carriage in pandemic Escherichia coli ST1193","volume":"10","author":"Wyrsch","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jgar.2024.11.020","article-title":"Emergent Escherichia coli of the highly virulent B2-ST1193 clone producing KPC-2 carbapenemase in ready-to-eat vegetables","volume":"41","author":"Dantas","year":"2025","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"D757","DOI":"10.1093\/nar\/gkae902","article-title":"EnteroBase in 2025: Exploring the genomic epidemiology of bacterial pathogens","volume":"53","author":"Dyer","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13099-019-0290-0","article-title":"Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: Recent reports","volume":"11","author":"Sarowska","year":"2019","journal-title":"Gut Pathog."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e00570-23","DOI":"10.1128\/jcm.00570-23","article-title":"Construction of the ETECFinder database for the characterization of enterotoxigenic Escherichia coli (ETEC) and revision of the VirulenceFinder web tool at the CGE website","volume":"62","author":"Scheutz","year":"2024","journal-title":"J. Clin. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Braz, V.S., Melchior, K., and Moreira, C.G. (2020). Escherichia coli as a multifaceted pathogenic and versatile bacterium. Front. Cell. Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.548492"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5811","DOI":"10.1038\/s41467-024-50268-2","article-title":"Population structure and antibiotic resistance of swine extraintestinal pathogenic Escherichia coli from China","volume":"15","author":"Li","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Santos, A.C.M., Santos, F.F., Silva, R.M., and Gomes, T.A.T. (2020). Diversity of hybrid- and hetero-pathogenic Escherichia coli and their potential implication in more severe diseases. Front. Cell. Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.00339"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.ijmm.2013.02.001","article-title":"Plasmids and the spread of resistance","volume":"303","author":"Carattoli","year":"2013","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_35","unstructured":"BrCAST (Breakpoint Tables for Interpretation of MICs and Zone Diameters, 2023). Breakpoint Tables for Interpretation of MICs and Zone Diameters, Version 13.0."},{"key":"ref_36","unstructured":"CLSI (2023). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Supplement VET01S, Clinical and Laboratory Standards Institute. [6th ed.]."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"160","DOI":"10.33086\/ijmlst.v5i2.4773","article-title":"Analysis of Purity and Concentration Escherichia coli DNA by Boiling Method Isolation with Addition of Proteinase-K and RNase","volume":"5","author":"Lesiani","year":"2023","journal-title":"Indones. J. Med. Lab. Sci. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1128\/AEM.66.10.4555-4558.2000","article-title":"Rapid and simple determination of the Escherichia coli phylogenetic group","volume":"66","author":"Clermont","year":"2000","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/8\/819\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:27:35Z","timestamp":1760034455000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/8\/819"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,10]]},"references-count":39,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["antibiotics14080819"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics14080819","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,10]]}}}