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Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior through a Visiting Senior Professor Scholarship","doi-asserted-by":"publisher","award":["88887.023263\/2024-00"],"award-info":[{"award-number":["88887.023263\/2024-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: Corynebacterium ulcerans is an emerging zoonotic pathogen capable of cau-sing diphtheria-like infections in humans. Objectives: we report, for the first time in Brazil, the detection and phenotypic\/genomic characterization of three atoxigenic ST-339 strains isolated from domestic animals, including one with a ciprofloxacin resistance profile linked to double GyrA mutations (S89L, D93G). Methods: species identification was performed by MALDI-TOF MS, followed by in vitro antimicrobial susceptibility testing, whole-genome sequencing, and bioinformatic analyses to predict virulence determinants, antimicrobial resistance genes, CRISPR\u2013Cas systems, mobile genetic elements, and in silico structural analysis as well as phylogenetic reconstruction. Results: whole-genome sequencing confirmed species identity, revealed high genetic similarity, and identified distinct phylogenetic subclades, suggesting potential international dissemination. Genomic analyses showed conserved virulence determinants, such as incomplete pilus clusters, iron acquisition systems, and the pld gene, with the absence of the tox gene. Molecular modeling and dynamics simulations indicated that GyrA mutations disrupt critical ciprofloxacin\u2013magnesium\u2013water interactions, reducing binding stability. Mobile genetic elements, prophages, and CRISPR\u2013Cas systems underscored the genomic plasticity of these isolates. Conclusions: these findings document a little-studied antimicrobial resistance mechanism in zoonotic C. ulcerans, highlighting the need for strengthened surveillance and further research on virulence and resistance, even in ato-xigenic strains.<\/jats:p>","DOI":"10.3390\/antibiotics14080843","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T07:50:46Z","timestamp":1755676246000},"page":"843","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Quinolone Resistance and Zoonotic Potential of Corynebacterium ulcerans from Domestic Animals in Brazil"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6773-0381","authenticated-orcid":false,"given":"Fernanda Diniz","family":"Prates","sequence":"first","affiliation":[{"name":"Operational Technical Nucleus, Microbiology, Hermes Pardini Institute, Vespasiano 33200-000, MG, Brazil"},{"name":"Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3293-8496","authenticated-orcid":false,"given":"Max Roberto Batista","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Operational Technical Nucleus, Microbiology, Hermes Pardini Institute, Vespasiano 33200-000, MG, Brazil"},{"name":"Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5297-7532","authenticated-orcid":false,"given":"Jailan","family":"da Silva Sousa","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6933-8961","authenticated-orcid":false,"given":"Lincoln","family":"de Oliveira Sant\u2019Anna","sequence":"additional","affiliation":[{"name":"Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, Rio de Janeiro State University, Rio de Janeiro 20550-013, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2975-1144","authenticated-orcid":false,"given":"Tayn\u00e1","family":"do Carmo Sant\u2019Anna Cardoso","sequence":"additional","affiliation":[{"name":"Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, Rio de Janeiro State University, Rio de Janeiro 20550-013, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Couto Calazans","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, Rio de Janeiro State University, Rio de Janeiro 20550-013, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7299-3724","authenticated-orcid":false,"given":"Siomar","family":"de Castro Soares","sequence":"additional","affiliation":[{"name":"Institute of Biological and Natural Sciences, Federal University of Tri\u00e2ngulo Mineiro, Uberaba 38025-350, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8031-9454","authenticated-orcid":false,"given":"Bruno Silva","family":"Andrade","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics and Computational Chemistry, Department of Biological Sciences, State University of Southwest of Bahia, Jequi\u00e9 45083-900, BA, Brazil"},{"name":"France\u2019s National Research Institute for Agriculture, Food and Environment, 75338 Rennes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5303-6395","authenticated-orcid":false,"given":"Louisy Sanches","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, Rio de Janeiro State University, Rio de Janeiro 20550-013, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4775-2280","authenticated-orcid":false,"given":"Vasco Ariston","family":"de Carvalho Azevedo","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, 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