{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:13:35Z","timestamp":1790144015717,"version":"4.0.1"},"reference-count":93,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"International Development Research Center (IDRC)","award":["110298-001"],"award-info":[{"award-number":["110298-001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>This study investigated the molecular and phenotypic characteristics of antimicrobial resistance in Escherichia coli isolates recovered from the ceca of healthy broilers in Tunisia. A total of 111 E. coli isolates were obtained from chicken samples collected at slaughterhouses and cultured on cefotaxime-supplemented MacConkey agar. All isolates exhibited a multidrug-resistant (MDR) phenotype, and 72.1% were confirmed as extended-spectrum \u03b2-lactamase (ESBL) producers. The most frequent \u03b2-lactamase gene was blaCTX-M-G1, followed by blaTEM and blaSHV. Carbapenem resistance genes (blaOXA-48 and blaIMP) were detected in 12.6% and 6.3% of isolates, respectively, while six isolates harbored the colistin resistance gene mcr-1. Among the tested virulence genes, fimH, traT, and iutA were the most prevalent, detected in over 70% of isolates. Class 1 integrons were present in 83% of isolates, and class 2 integrons in 39.6%, with gene cassettes encoding resistance to trimethoprim (dfrA) and streptomycin (aadA). These findings highlight the widespread presence of MDR and ESBL-producing E. coli strains with virulence traits and integrons in poultry, underscoring the risk of transmission to humans. This study provides essential data supporting the implementation of integrated surveillance strategies in line with the One Health approach.<\/jats:p>","DOI":"10.3390\/antibiotics14090931","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T08:56:46Z","timestamp":1758013006000},"page":"931","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Phenotypic and Molecular Study of Multidrug-Resistant Escherichia coli Isolates Expressing Diverse Resistance and Virulence Genes from Broilers in Tunisia"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7180-6787","authenticated-orcid":false,"given":"Ghassan","family":"Tayh","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, National School of Veterinary Medicine, University of Manouba, LR16AGR01, Sidi Thabet, Ariana 2020, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Nsibi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, National School of Veterinary Medicine, University of Manouba, LR16AGR01, Sidi Thabet, Ariana 2020, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4677-7547","authenticated-orcid":false,"given":"Khaled","family":"Abdallah","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, National School of Veterinary Medicine, University of Manouba, LR16AGR01, Sidi Thabet, Ariana 2020, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8208-0121","authenticated-orcid":false,"given":"Omar","family":"Abbes","sequence":"additional","affiliation":[{"name":"DICK Company, Poulina Group Holding, Ben Arous 2034, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Fliss","sequence":"additional","affiliation":[{"name":"Department of Food Sciences and Nutrition, University of Laval, Qu\u00e9bec City, QC G1K 7P4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilia","family":"Messadi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, National School of Veterinary Medicine, University of Manouba, LR16AGR01, Sidi Thabet, Ariana 2020, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5698","DOI":"10.1128\/AEM.70.9.5698-5700.2004","article-title":"Large-scale population structure of human commensal Escherichia coli isolates","volume":"70","author":"Grenet","year":"2004","journal-title":"Appl. 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