{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T15:05:12Z","timestamp":1772291112611,"version":"3.50.1"},"reference-count":69,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n (SECIHTI) of Mexico through the call for proposals Ciencia de Frontera 2023","award":["CF-2023-G-645"],"award-info":[{"award-number":["CF-2023-G-645"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: Acinetobacter baumannii is a highly concerning pathogen in hospital settings, responsible for severe infections such as ventilator-associated pneumonia, urinary tract infections, and meningitis. Its remarkable genetic plasticity facilitates the rapid acquisition of antibiotic resistance, significantly complicating treatment and increasing mortality rates. As multidrug-resistant (MDR) infections continue to rise, phage therapy emerges as a viable alternative. Methods: This study reports the isolation and characterization of Acinetobacter phage vB_AbaM_A72 from stagnant water in Jalisco, Mexico. Results: Transmission electron microscopy revealed a myovirus-like morphology with an icosahedral head (91.32 \u00b1 0.12 nm) and a contractile tail (123.77 \u00b1 0.19 nm). The phage exhibited high environmental resilience, tolerating temperatures up to 60 \u00b0C and pH ranging from 5 to 11. Notably, A72 demonstrated a narrow host range but effectively inhibited the growth of an MDR A. baumannii strain for at least 12 h across different multiplicities of infection. Whole-genome sequencing confirmed the absence of virulence, antibiotic resistance, or lysogeny-associated genes. Comparative genomic analysis identified A72 as the first member of a newly described Obolenskvirus species, sharing only 76.4% similarity with its closest relatives. Conclusions: These findings underscore the importance of fully characterizing novel bacteriophages to expand therapeutic libraries and reinforce the feasibility of phage therapy as a promising approach against MDR A. baumannii infections.<\/jats:p>","DOI":"10.3390\/antibiotics14100961","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T08:56:44Z","timestamp":1758790604000},"page":"961","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Newly Discovered Obolenskvirus Phage with Sustained Lytic Activity Against Multidrug-Resistant Acinetobacter baumannii"],"prefix":"10.3390","volume":"14","author":[{"given":"Eduardo","family":"Vera-Jauregui","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda Microbiana y Alimentaria, Departamento de Ciencias B\u00e1sicas, Divisi\u00f3n de Desarrollo Biotecnol\u00f3gico, Centro Universitario de la Ci\u00e9nega, Universidad de Guadalajara, Av. Universidad 1115, Col. Lindavista, Ocotlan 47820, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5293-9704","authenticated-orcid":false,"given":"Mar\u00eda Guadalupe","family":"Avila-Novoa","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda Microbiana y Alimentaria, Departamento de Ciencias B\u00e1sicas, Divisi\u00f3n de Desarrollo Biotecnol\u00f3gico, Centro Universitario de la Ci\u00e9nega, Universidad de Guadalajara, Av. Universidad 1115, Col. Lindavista, Ocotlan 47820, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6664-1108","authenticated-orcid":false,"given":"Berenice","family":"Gonz\u00e1lez-Torres","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional para la Investigaci\u00f3n en Inocuidad Alimentaria (LANIIA), Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo (CIAD), Carretera a Eldorado Km 5.5, Campo El Diez, Culiacan 80110, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8982-4852","authenticated-orcid":false,"given":"Pedro Javier","family":"Guerrero-Medina","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda Microbiana y Alimentaria, Departamento de Ciencias B\u00e1sicas, Divisi\u00f3n de Desarrollo Biotecnol\u00f3gico, Centro Universitario de la Ci\u00e9nega, Universidad de Guadalajara, Av. Universidad 1115, Col. Lindavista, Ocotlan 47820, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5071-8270","authenticated-orcid":false,"given":"Cristobal","family":"Chaidez","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional para la Investigaci\u00f3n en Inocuidad Alimentaria (LANIIA), Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo (CIAD), Carretera a Eldorado Km 5.5, Campo El Diez, Culiacan 80110, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6134-4702","authenticated-orcid":false,"given":"Irvin","family":"Gonz\u00e1lez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional para la Investigaci\u00f3n en Inocuidad Alimentaria (LANIIA), Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo (CIAD), Carretera a Eldorado Km 5.5, Campo El Diez, Culiacan 80110, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2994-4358","authenticated-orcid":false,"given":"Jean Pierre","family":"Gonz\u00e1lez-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional para la Investigaci\u00f3n en Inocuidad Alimentaria (LANIIA), Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo (CIAD), Carretera a Eldorado Km 5.5, Campo El Diez, Culiacan 80110, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6014-4602","authenticated-orcid":false,"given":"Melesio","family":"Guti\u00e9rrez-Lomel\u00ed","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda Microbiana y Alimentaria, Departamento de Ciencias B\u00e1sicas, Divisi\u00f3n de Desarrollo Biotecnol\u00f3gico, Centro Universitario de la Ci\u00e9nega, Universidad de Guadalajara, Av. Universidad 1115, Col. Lindavista, Ocotlan 47820, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Urban-Chmiel, R., Marek, A., St\u0119pie\u0144-Py\u015bniak, D., Wieczorek, K., Dec, M., Nowaczed, A., and Osek, J. (2022). Antibiotic Resistance in Bacteria\u2014A Review. Antibiotics, 11.","DOI":"10.3390\/antibiotics11081079"},{"key":"ref_2","first-page":"e65","article-title":"Consenso latinoamericano para definir, categorizar y notificar pat\u00f3genos multirresistentes, con resistencia extendida o panresistentes","volume":"43","author":"Galas","year":"2019","journal-title":"Rev. Panam. 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