{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:26:44Z","timestamp":1789154804672,"version":"build-2803163510"},"reference-count":44,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: The aim of the present study was to evaluate the antimicrobial, antibiofilm, anti-quorum sensing, and cytotoxic activities of the essential oils extracted from the leaves of Dorystoechas hastata Boiss &amp; Helder. ex Bentham (Lamiaceae) (DHL-EO) as well as to determine the chemical composition of the essential oils obtained from both the leaves and roots. Methods: The essential oils of the root and leaf were extracted by the hydrodistillation method. The chemical composition of the two oils was determined by Gas Chromatography\u2013Mass Spectrometry (GC-MS). The antimicrobial activity of DHL-EO was determined against Gram-positive, Gram-negative bacteria, and various Candida species using the broth microdilution method. Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum ATCC 12472 were used for antibiofilm and anti-quorum sensing activities, respectively. The cytotoxic activity of the DHL-EO was examined by MTT assay. Results: Eucalyptol (21.3%), 2-bornanone (17.0%), and \u03b1-pinene (10.3%) were the main compounds of the DHL-EO. The root essential oil (DHR-EO) had trans-ferruginol (19.2%), guaiol (14.1%), and ar-abietatriene (14.0%) as the main components. The DHL-EO displayed weak and moderate antimicrobial activity. The DHL-EO showed moderate antibacterial activity against Staphylococcus aureus ATCC 29213 (methicillin-susceptible, MSSA) and S. aureus ATCC 43300 (methicillin-resistant, MRSA), with a MIC value of 12.5 mg\/mL. The DHL-EO exhibited the strongest antifungal activity against Candida parapsilosis RSKK 994, with a MIC value of 0.78 mg\/mL. It also demonstrated antifungal activity against C. parapsilosis ATCC 22019 and Candida krusei RSKK 3016, with MIC values of 3.12 mg\/mL. The DHL-EO showed antibiofilm activity in a concentration-dependent manner, particularly at higher concentrations, and inhibited violacein production in a dose-dependent manner, with anti-quorum sensing activity. The DHL-EO displayed moderate cytotoxic activity against MCF-7 (IC50: 110.3 \u03bcg\/mL) and A549 (IC50: 120.4 \u03bcg\/mL) cell lines. Conclusions: The chemical composition of DHL-EO and DHR-EO showed qualitative and quantitative differences from each other in the present study. The essential oil of the leaves showed moderate cytotoxic and antibacterial activities.<\/jats:p>","DOI":"10.3390\/antibiotics14101019","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T07:51:28Z","timestamp":1760428288000},"page":"1019","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Antimicrobial: Antibiofilm, Anti-Quorum Sensing and Cytotoxic Activities of Dorystoechas hastata Boiss &amp; Heldr. ex Bentham Essential Oil"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7434-3175","authenticated-orcid":false,"given":"Timur Hakan","family":"Barak","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basar","family":"Karaca","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Ankara University, 06560 Ankara, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huseyin","family":"Servi","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Istanbul Yeni Y\u00fczy\u0131l University, 34010 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7976-7057","authenticated-orcid":false,"given":"Simge","family":"Kara Ertekin","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul Yeni Y\u00fczy\u0131l University, 34010 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tu\u011fba Buse","family":"\u015eent\u00fcrk","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhittin","family":"Dinc","sequence":"additional","affiliation":[{"name":"Department of Mathematic and Science Education, Ahmet Kele\u015fo\u011flu Faculty of Education, Necmettin Erbakan University, 42090 Konya, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatice","family":"Ustuner","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Akdeniz University, 07070 Antalya, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3760-1996","authenticated-orcid":false,"given":"Mujde","family":"Eryilmaz","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/S0305-1978(03)00037-1","article-title":"Chemodiversity of exudate flavonoids in some members of the Lamiaceae Biochem","volume":"31","author":"Roitman","year":"2003","journal-title":"Syst. 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