{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:22:24Z","timestamp":1790389344400,"version":"4.1.0"},"reference-count":116,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Antimicrobial resistance is a critical global health challenge. Among resistant pathogens, the group of bacteria collectively referred to as ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) is of particular concern due to their ability to evade multiple classes of antimicrobials. This study aimed to investigate the occurrence and resistance patterns of ESKAPE bacteria in wild birds from Northern and Central Italy sites, and to assess the presence of other bacteria of public health relevance. Methods: Cloacal swabs were collected from 141 wild birds. Samples were processed on selective and differential media, and bacterial identification was performed using Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry. Antimicrobial susceptibility was evaluated through Minimum Inhibitory Concentration assays and interpreted according to international guidelines. Results: Thirty-seven isolates belonging to the ESKAPE group were identified: E. faecium (n = 10), K. pneumoniae (n = 9), P. aeruginosa (n = 8), Enterobacter spp. (n = 7), S. aureus (n = 2), and A. baumannii (n = 1). Multidrug-resistant isolates were observed among K. pneumoniae and Enterobacter hormaechei. Escherichia coli, although not included in the ESKAPE group, was frequently detected and often co-isolated with clinically relevant bacteria, highlighting its potential role as a reservoir of resistance genes. Conclusions: Wild birds can harbor resistant bacteria of clinical importance, including multidrug-resistant ESKAPE species. Their presence in avian populations underscores the role of wildlife in the environmental dissemination of antimicrobial resistance, with implications for both animal and human health.<\/jats:p>","DOI":"10.3390\/antibiotics14101025","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T14:34:15Z","timestamp":1760452455000},"page":"1025","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Looking for ESKAPE Bacteria: Occurrence and Phenotypic Antimicrobial Resistance Profiles in Wild Birds from Northern and Central Italy Sites"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6157-7258","authenticated-orcid":false,"given":"Guido","family":"Grilli","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1496-5195","authenticated-orcid":false,"given":"Maria Cristina","family":"Rapi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1106-2130","authenticated-orcid":false,"given":"Laura","family":"Musa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giacomo","family":"Di Giacinto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8773-838X","authenticated-orcid":false,"given":"Fabrizio","family":"Passamonti","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06100 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Raimondi","sequence":"additional","affiliation":[{"name":"Wildlife Rescue Centres (CRAS)\u2014\u201cBosco WWF di Vanzago\u201d, Via delle 3 Campane, 20043 Vanzago, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriana","family":"Cianca","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06100 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Pia","family":"Franciosini","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06100 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"ref_1","unstructured":"WHO (2025, April 14). 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