{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:29:38Z","timestamp":1789684178185,"version":"4.0.1"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Staphylococcus pseudintermedius (S. pseudintermedius) is an opportunistic pathogen frequently isolated from dogs, involved in a wide range of infections, particularly otitis externa. Increasing antimicrobial resistance (AMR), including methicillin-resistant S. pseudintermedius (MRSP), poses significant challenges for veterinary and potentially human health. This study aimed to assess the prevalence and antimicrobial resistance profiles of S. pseudintermedius in dogs with otitis externa compared to clinically healthy dogs. Methods: Between 2022 and 2025, samples were collected from 400 dogs with otitis externa and 360 healthy dogs in veterinary clinics from Timi\u0219oara. Ear swabs were processed by conventional microbiological techniques and confirmed using MALDI-TOF MS. Antimicrobial susceptibility was tested using the VITEK\u00ae 2 Compact system, following CLSI VET01, Fifth Edition (2018) standards. Fourteen antimicrobials from 11 classes were evaluated. Results: S. pseudintermedius was isolated in 40% of dogs with otitis externa and in 21.1% of healthy dogs. The highest resistance in both groups was observed to tetracycline (37.5% and 25%, respectively). No resistance was recorded to linezolid, vancomycin, teicoplanin, tigecycline, or fusidic acid. MRSP strains were identified in 1.2% of dogs with otitis, displaying multidrug resistance (MDR). MDR strains were also detected in 8.7% of diseased and 4% of healthy dogs, indicating the potential for subclinical reservoirs. Conclusions: The findings highlight the notable prevalence and AMR of S. pseudintermedius in both symptomatic and asymptomatic dogs. The detection of MRSP and MDR strains emphasizes the need for prudent antibiotic use and continuous AMR surveillance in veterinary medicine to mitigate zoonotic risks and preserve antimicrobial efficacy.<\/jats:p>","DOI":"10.3390\/antibiotics14101027","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T06:06:32Z","timestamp":1760508392000},"page":"1027","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Antimicrobial Resistance Profile of Staphylococcus pseudintermedius Isolated from Dogs with Otitis Externa and Healthy Dogs: Veterinary and Zoonotic Implications"],"prefix":"10.3390","volume":"14","author":[{"given":"Ionela","family":"Popa","sequence":"first","affiliation":[{"name":"Department of Semiology, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8913-4942","authenticated-orcid":false,"given":"Ionica","family":"Iancu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vlad","family":"Iorgoni","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5859-5057","authenticated-orcid":false,"given":"Janos","family":"Degi","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2531-6469","authenticated-orcid":false,"given":"Alexandru","family":"Gligor","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6057-882X","authenticated-orcid":false,"given":"Kalman","family":"Imre","sequence":"additional","affiliation":[{"name":"Department of Food Safety and Hygiene, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emil","family":"T\u00eerziu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7112-7680","authenticated-orcid":false,"given":"Timea","family":"Bochi\u0219","sequence":"additional","affiliation":[{"name":"Department of Semiology, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u0103lin","family":"Pop","sequence":"additional","affiliation":[{"name":"Department of Semiology, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana-Maria","family":"Plotuna","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9380-1985","authenticated-orcid":false,"given":"Paula","family":"Nistor","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marius","family":"Pentea","sequence":"additional","affiliation":[{"name":"Department of Anatomy, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7118-6488","authenticated-orcid":false,"given":"Viorel","family":"Herman","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ileana","family":"Nichita","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lynch, S.A., and Helbig, K.J. (2021). The Complex Diseases of Staphylococcus pseudintermedius in Canines: Where to Next?. Vet. Sci., 8.","DOI":"10.3390\/vetsci8010011"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Vi\u00f1es, J., F\u00e0bregas, N., P\u00e9rez, D., Cusc\u00f3, A., Fonticoba, R., Francino, O., Ferrer, L., and Migura-Garcia, L. (2022). Concordance between Antimicrobial Resistance Phenotype and Genotype of Staphylococcus pseudintermedius from Healthy Dogs. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111625"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.vetmic.2018.09.013","article-title":"Comparative genomics of phenotypic antimicrobial resistances in methicillin-resistant Staphylococcus pseudintermedius of canine origin","volume":"225","author":"Wegener","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1128\/JCM.01288-15","article-title":"Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands","volume":"54","author":"Duim","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Lee, G.Y., and Yang, S.J. (2020). Comparative assessment of genotypic and phenotypic correlates of Staphylococcus pseudintermedius strains isolated from dogs with otitis externa and healthy dogs. Comp. Immunol. Microbiol. Infect. Dis., 70.","DOI":"10.1016\/j.cimid.2019.101376"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Popa, I., Imre, K., Morar, A., Iancu, I., Iorgoni, V., Bochi\u0219, T., Pop, C., Gligor, A., Florea, T., and Popa, S.A. (2025). Questionnaire-Based Survey Regarding the Rational Usage of Antimicrobial Agents in Food-Producing Animals in Romania. Vet. Sci., 12.","DOI":"10.3390\/vetsci12050408"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Parasana, D.K., Kalyani, I.H., Kachchhi, A.V., Koringa, P.G., Makwana, P.M., Patel, D.R., Ramani, U.V., Javia, B.B., and Ghodasara, S.N. (2025). Profiling of antimicrobial resistance genes from Staphylococcus pseudintermedius isolated from dogs with pyoderma using whole genome sequencing. Comp. Immunol. Microbiol. Infect. Dis., 116.","DOI":"10.1016\/j.cimid.2024.102288"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ertas Onmaz, N., Demirezen Yilmaz, D., Imre, K., Morar, A., Gungor, C., Yilmaz, S., Gundog, D.A., Dishan, A., Herman, V., and Gungor, G. (2022). Green Synthesis of Gold Nanoflowers Using Rosmarinus officinalis and Helichrysum italicum Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111466"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Abdallah, R., Mostafa, N.Y., Kirrella, G.A.K., Gaballah, I., Imre, K., Morar, A., Herman, V., Sallam, K.I., and Elshebrawy, H.A. (2023). Antimicrobial Effect of Moringa oleifera Leaves Extract on Foodborne Pathogens in Ground Beef. Foods, 12.","DOI":"10.3390\/foods12040766"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.vetmic.2019.04.002","article-title":"Molecular characterisation of methicillin-resistant Staphylococcus pseudintermedius from dogs and the description of their SCCmec elements","volume":"233","author":"Krapf","year":"2019","journal-title":"Vet. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Viegas, F.M., Santana, J.A., Silva, B.A., Xavier, R.G.C., Bonisson, C.T., C\u00e2mara, J.L.S., Renn\u00f3, M.C., Cunha, J.L.R., Figueiredo, H.C.P., and Lobato, F.C.F. (2022). Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0269422"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Morais, C., Costa, S.S., Leal, M., Ramos, B., Andrade, M., Ferreira, C., Abrantes, P., Pomba, C., and Couto, I. (2023). Genetic diversity and antimicrobial resistance profiles of Staphylococcus pseudintermedius associated with skin and soft-tissue infections in companion animals in Lisbon, Portugal. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1167834"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1093\/jac\/dkr367","article-title":"Review on methicillin-resistant Staphylococcus pseudintermedius","volume":"66","author":"Catry","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Moses, I.B., Santos, F.F., and Gales, A.C. (2023). Human colonization and infection by Staphylococcus pseudintermedius: An emerging and underestimated zoonotic pathogen. Microorganisms, 11.","DOI":"10.3390\/microorganisms11030581"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"43","DOI":"10.2478\/macvetrev-2023-0012","article-title":"Otitis externa in dogs: Distribution and antimicrobial susceptibility patterns of Staphylococcus spp. isolates","volume":"46","author":"Hassan","year":"2023","journal-title":"Mac. Vet. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/j.1365-3164.2009.00842.x","article-title":"Species distribution and antimicrobial susceptibility of staphylococci isolated from canine otitis externa","volume":"21","author":"Penna","year":"2010","journal-title":"Vet. Dermatol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.apjtb.2015.11.012","article-title":"An update on microbiological causes of canine otitis externa in Campania Region, Italy","volume":"6","author":"Nocera","year":"2016","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Rosales, R.S., Ram\u00edrez, A.S., Moya-Gil, E., de la Fuente, S.N., Su\u00e1rez-P\u00e9rez, A., and Poveda, J.B. (2024). Microbiological survey and evaluation of antimicrobial susceptibility patterns of microorganisms obtained from suspect cases of canine otitis externa in Gran Canaria, Spain. Animals, 14.","DOI":"10.3390\/ani14050742"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"449","DOI":"10.24425\/pjvs.2023.145052","article-title":"Prevalence of the microbiological causes of canine otitis externa and the antibiotic susceptibility of the isolated bacterial strains","volume":"26","author":"Tesin","year":"2023","journal-title":"Pol. J. Vet. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e121","DOI":"10.1017\/S0950268818003278","article-title":"Antimicrobial resistance patterns of bacteria isolated from dogs with otitis","volume":"147","author":"Cazeau","year":"2019","journal-title":"Epidemiol. Infect."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e20170738","DOI":"10.1590\/0103-8478cr20170738","article-title":"Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius in dogs with otitis externa","volume":"48","author":"Scherer","year":"2018","journal-title":"Ci\u00eancia Rural"},{"key":"ref_22","first-page":"153","article-title":"Antimicrobial susceptibility of Staphylococcus aureus and Staphylococcus pseudintermedius isolated from various animals","volume":"52","author":"Rubin","year":"2011","journal-title":"Can. Vet. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1089\/mdr.2009.0865","article-title":"Antimicrobial resistance in Staphylococcus pseudintermedius in the Norwegian dog population","volume":"15","author":"Sunde","year":"2009","journal-title":"Microb. Drug Resist."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1111\/avj.12007","article-title":"Identification and antibiotic susceptibility of bacterial isolates from dogs with otitis externa in Australia","volume":"91","author":"Bugden","year":"2013","journal-title":"Aust. Vet. J."},{"key":"ref_25","unstructured":"European Medicines Agency (2025, July 20). Advice on Designation of Antimicrobials or Groups of Antimicrobials Reserved for Treatment of Certain Infections in Humans in Relation to Implementing Measures Under Article 37(5) of Regulation (EU) 2019\/6 on Veterinary Medicinal Products. Available online: https:\/\/food.ec.europa.eu\/system\/files\/2022-03\/ah_vet-med_imp-reg-2019-06_ema-advice_art-37-5.pdf."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Papi\u0107, B., Golob, M., Zdovc, I., Ku\u0161ar, D., and Avber\u0161ek, J. (2021). Genomic insights into the emergence and spread of methicillin-resistant Staphylococcus pseudintermedius in veterinary clinics. Vet. Microbiol., 258.","DOI":"10.1016\/j.vetmic.2021.109119"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Iorgoni, V., Iancu, I., Popa, I., Gligor, A., Orghici, G., Sicoe, B., Dreghiciu, C., Purec, D., Nistor, P., and Florea, B. (2025). First Report of Respiratory Infection Caused by Multidrug-Resistant Escherichia coli in an Ostrich in Romania. Antibiotics, 14.","DOI":"10.3390\/antibiotics14040354"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"276-e55","DOI":"10.1111\/j.1365-3164.2012.01056.x","article-title":"Antimicrobial resistance of Staphylococcus pseudintermedius","volume":"23","author":"Kadlec","year":"2012","journal-title":"Vet. Dermatol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1177\/104063871102300227","article-title":"Prevalence, sites of colonization, and antimicrobial resistance among Staphylococcus pseudintermedius isolated from healthy dogs in Saskatoon, Canada","volume":"23","author":"Rubin","year":"2011","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.cvsm.2012.09.003","article-title":"Antimicrobial resistance in staphylococci in small animals","volume":"43","author":"Cain","year":"2013","journal-title":"Vet. Clin. North America. Small Anim. Pract."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.jgar.2025.02.010","article-title":"Antimicrobial susceptibility and genetic diversity of staphylococcus pseudintermedius isolated from companion animals and human clinical patients in Japan: Potential zoonotic implications","volume":"42","author":"Usui","year":"2025","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Srednik, M.E., Perea, C.A., Giacoboni, G.I., Hicks, J.A., Foxx, C.L., Harris, B., and Schlater, L.K. (2023). Genomic Features of Antimicrobial Resistance in Staphylococcus pseudintermedius Isolated from Dogs with Pyoderma in Argentina and the United States: A Comparative Study. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241411361"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1093\/jac\/dkq037","article-title":"Assessing the antimicrobial susceptibility of bacteria obtained from animals","volume":"65","author":"Schwarz","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"372","DOI":"10.37358\/MP.18.3.5032","article-title":"Phenotypic Study and Sensitivity to Anti-Infective Chemotherapy of Bacterial Strains Isolated from Cutaneous-Mucosal Infections","volume":"55","author":"Velimirovici","year":"2018","journal-title":"Mater. Plast."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3505","DOI":"10.1007\/s11259-024-10508-8","article-title":"Methicillin-Resistant Staphylococcus pseudintermedius: Epidemiological Changes, Antibiotic Resistance, and Alternative Therapeutic Strategies","volume":"48","author":"Nocera","year":"2024","journal-title":"Vet. Res. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Feuer, L., Frenzer, S.K., Merle, R., B\u00e4umer, W., L\u00fcbke-Becker, A., Klein, B., and Bartel, A. (2024). Comparative Analysis of Methicillin-Resistant Staphylococcus pseudintermedius Prevalence and Resistance Patterns in Canine and Feline Clinical Samples: Insights from a Three-Year Study in Germany. Antibiotics, 13.","DOI":"10.3390\/antibiotics13070660"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Tanveer, M., Ntakiyisumba, E., Hirwa, F., Yoon, H., Oh, S.-I., Kim, C., Kim, M.H., Yoon, J.-S., and Won, G. (2024). Prevalence of Bacterial Pathogens Isolated from Canines with Pyoderma and Otitis Externa in Korea: A Systematic Review and Meta-Analysis. Vet. Sci., 11.","DOI":"10.3390\/vetsci11120656"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wang, Q., Chen, S., Ma, S., Jiao, Y., Hong, H., Wang, S., Huang, W., An, Q., Song, Y., and Dang, X. (2025). Antimicrobial Resistance and Risk Factors of Canine Bacterial Skin Infections. Pathogens, 14.","DOI":"10.3390\/pathogens14040309"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Iorgoni, V., Iancu, I., Popa, I., Gligor, A., Orghici, G., Sicoe, B., Badea, C., Dreghiciu, C., Luca, I., and Nistor, P. (2025). The First Report of a Pulmonary Abscess Due to Streptococcus intermedius in Rabbits in Romania. Microorganisms, 13.","DOI":"10.3390\/microorganisms13040769"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Iancu, I., Popa, S.A., Degi, J., Gligor, A., Popa, I., Iorgoni, V., Nistor, P., Imre, K., Nichita, I., and Herman, V. (2025). Aerobic uterine pathogens in dairy cattle: Surveillance and antimicrobial resistance profiles in postpartum endometritis. Antibiotics, 14.","DOI":"10.3390\/antibiotics14070650"},{"key":"ref_41","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2018). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals, Clinical and Laboratory Standards Institute. [5th ed.]. CLSI document VET01."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/10\/1027\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T06:35:24Z","timestamp":1760510124000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/10\/1027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,14]]},"references-count":41,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["antibiotics14101027"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics14101027","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,14]]}}}