{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:24:38Z","timestamp":1767626678705,"version":"3.46.0"},"reference-count":77,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["305003\/2022-4"],"award-info":[{"award-number":["305003\/2022-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Science and Technology in Human Pathogenic Fungi","award":["FUNVIR-405934\/2022-0"],"award-info":[{"award-number":["FUNVIR-405934\/2022-0"]}]},{"DOI":"10.13039\/501100005283","name":"Cearense Foundation to Support Scientific and Technological Development","doi-asserted-by":"publisher","award":["PVS-0215 00099.01.00\/23"],"award-info":[{"award-number":["PVS-0215 00099.01.00\/23"]}],"id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"name":"FUNDECT (Foundation for the Support of the Development of Education"},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives Plant lectins have emerged as potential antifungal molecules, where the carbohydrate recognition domain (CRD) is possibly the main mode of action of these proteins. Previously, we saw that the lectin extracted from the seeds of Dioclea violacea (DVL) has anti-candida activity against Candida krusei cells by acting to inhibit ergosterol biosynthesis, cell wall deformation, and deregulation of the redox system. Methods We have now confirmed this anti-candida activity by proteomic analysis, with the expression of proteins that show us how C. krusei cells respond to this treatment. Results A total of 395 proteins were identified: 142 proteins exclusively found in untreated C. krusei cells and 245 proteins exclusive to DVL-treated cells. Eight proteins were detected in both conditions. Six displayed positive accumulation (fold change &gt; 1.5), one exhibited negative accumulation (fold change &lt; 0.5). We observed the expression of proteins related to cell wall remodeling; alteration of energy metabolism, suggesting a metabolic adaptation to stress; oxidative stress was responded to through the expression of proteins with antioxidant action, in addition to identifying multidrug transport proteins that are often involved in the process of antifungal resistance and sterol transport to the membrane. Conclusions Our results show the complexity of adaptive responses of C. krusei cells to treatment with DVL, elucidating new mechanisms of resistance and paving the way for the development of more effective and innovative antifungal therapies.<\/jats:p>","DOI":"10.3390\/antibiotics14121228","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T16:37:02Z","timestamp":1764952622000},"page":"1228","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["DVL, a Lectin from Dioclea violacea Seeds, Disturbs the Proteomic Profile of Candida krusei, Leading to Cell Death"],"prefix":"10.3390","volume":"14","author":[{"given":"Rom\u00e9rio R. S.","family":"Silva","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1 (UFC), Fortaleza 60451-970, CE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rayara J. P.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1 (UFC), Fortaleza 60451-970, CE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria H. C.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1 (UFC), Fortaleza 60451-970, CE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana L. E.","family":"Santos","sequence":"additional","affiliation":[{"name":"Medical School, Federal University of Cariri, Barbalha 63180-000, CE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00f4mulo F.","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Department of Fisheries Engineering, Federal University of Cear\u00e1 (UFC), Fortaleza 60440-900, CE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5988-5868","authenticated-orcid":false,"given":"Celso S.","family":"Nagano","sequence":"additional","affiliation":[{"name":"Department of Fisheries Engineering, Federal University of Cear\u00e1 (UFC), Fortaleza 60440-900, CE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2524-4434","authenticated-orcid":false,"given":"Pedro F. N.","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics Applied to Human Health, Center for Drug Research and Development (NPDM), Federal University of Cear\u00e1 (UFC), Fortaleza 60355-636, CE, Brazil"},{"name":"National Institute of Science and Technology in Human Pathogenic Fungi (FunVir), Ribeir\u00e3o Preto 14040-903, SP, Brazil"},{"name":"Visiting Researcher at the Cearense Foundation to Support Scientific and Technological Development, Fortaleza 60325-452, CE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9792-0369","authenticated-orcid":false,"given":"Claudener S.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Center for Agricultural Sciences and Biodiversity, Federal University of Cariri, Crato 63130-025, CE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15202","DOI":"10.3390\/molecules200815202","article-title":"Protein-Carbohydrate Interactions, and Beyond \u2026","volume":"20","year":"2015","journal-title":"Molecules"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1080\/07352689891304276","article-title":"Plant Lectins: A Composite of Several Distinct Families of Structurally and Evolutionary Related Proteins with Diverse Biological Roles","volume":"17","author":"Peumans","year":"1998","journal-title":"CRC Crit. 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