{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:51:42Z","timestamp":1788245502416,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Funda\u00e7\u00e3o de Apoio ao Desenvolvimento do Ensino"},{"name":"Ci\u00eancia e Tecnologia do Estado de Mato Grosso do Sul"},{"DOI":"10.13039\/501100005668","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Distrito Federal","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004809","name":"Financiadora de Estudos e Projetos","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The growing global threat of antimicrobial resistance (AMR), particularly in cutaneous wound infections, represents a significant clinical and economic challenge. Biofilm formation by multidrug-resistant pathogens, such as Acinetobacter baumannii, often complicates healing and leads to therapeutic failure. Antimicrobial peptides (AMPs) are a promising alternative to conventional antibiotics due to their potent membrane-disrupting mechanism of action and lower propensity to induce resistance. Background\/Objectives: This study aimed to evaluate the antibacterial, antibiofilm, and in vivo efficacy of four snake venom-derived cathelicidin-like peptides\u2014Btn (15-34) and BotrAMP14 from Bothrops atrox, and Ctn (15-34) and CrotAMP14 from Crotalus durissus\u2014against multidrug-resistant A. baumannii, Escherichia coli, and Pseudomonas aeruginosa clinical isolates from skin infections, with emphasis on A. baumannii, a WHO priority pathogen. Methods: Minimal Inhibitory Concentration (MIC), Minimal Bactericidal Concentration (MBC), and Minimal Biofilm Inhibitory Concentration (MBIC) were determined against A. baumannii, Escherichia coli, and Pseudomonas aeruginosa. Time-kill kinetics, hemolytic activity, and cytotoxicity assays were performed. A murine skin wound infection model was established to evaluate in vivo antibacterial efficacy and safety. Results: MIC\/MBC values ranged from 0.78 to 25 \u00b5M against planktonic cells. In comparison, MBIC ranged from 1.56 to 12.5 \u00b5M against biofilms. BotrAMP14 eradicated A. baumannii within 4 min, while CrotAMP14 achieved bactericidal action in 20 min at 1.56 \u00b5M. Both peptides exhibited no hemolytic activity up to 128 \u00b5M and low cytotoxicity (IC50 &gt; 128 \u00b5M). In vivo, BotrAMP14 and CrotAMP14 demonstrated significant antibacterial activity at 24 h and 48 h post-infection, respectively, surpassing that of meropenem. Conclusions: These findings suggest that BotrAMP14 and CrotAMP14 are promising topical antimicrobial agents for managing multidrug-resistant skin infections and may help address the urgent need for alternative therapies against antibiotic-resistant pathogens.<\/jats:p>","DOI":"10.3390\/antibiotics15010077","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T09:13:01Z","timestamp":1768209181000},"page":"77","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cathelicidin-like Peptide for Resistant Acinetobacter baumannii Control"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3126-029X","authenticated-orcid":false,"given":"Elizabete de Souza","family":"C\u00e2ndido","sequence":"first","affiliation":[{"name":"Centro de An\u00e1lises Prote\u00f4micas e Bioqu\u00edmicas, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Gen\u00f4micas e Biotecnologia, Universidade Cat\u00f3lica de Bras\u00edlia, Bras\u00edlia 71966-700, DF, Brazil"},{"name":"S-Inova Biotech, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6381-3779","authenticated-orcid":false,"given":"Danieli Fernanda","family":"Buccini","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizangela de Barros","family":"Miranda","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3032-5431","authenticated-orcid":false,"given":"Regina Meneses","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7951-9125","authenticated-orcid":false,"given":"Amanda Loren de Oliveira","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9523-4843","authenticated-orcid":false,"given":"Valentina","family":"Nieto-Mar\u00edn","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2959-4984","authenticated-orcid":false,"given":"Ana Paula Ferreira","family":"Leal","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samilla Beatriz","family":"Rezende","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, 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P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Gen\u00f4micas e Biotecnologia, Universidade Cat\u00f3lica de Bras\u00edlia, Bras\u00edlia 71966-700, DF, Brazil"},{"name":"S-Inova Biotech, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/s41522-021-00243-2","article-title":"Burns and Biofilms: Priority Pathogens and in Vivo Models","volume":"7","author":"Maslova","year":"2021","journal-title":"NPJ Biofilms Microbiomes"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1038\/s42003-021-01956-y","article-title":"Bacteria-Specific pro-Photosensitizer Kills Multidrug-Resistant Staphylococcus Aureus and Pseudomonas 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