{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T10:39:05Z","timestamp":1771324745389,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"United States Department of Agriculture, National Institute of Food and Agriculture","award":["2018-68006-28103"],"award-info":[{"award-number":["2018-68006-28103"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Antimicrobial resistance in food\u2013animal environments threatens sustainable production and public health, yet small farms remain poorly characterized as potential reservoirs of antimicrobial resistant bacteria. To address this, we investigated the prevalence and antimicrobial resistance profiles of Escherichia coli, Klebsiella spp., and Enterococcus spp. from small-scale cattle farms in Tennessee, USA. Methods: Over one year, 153 environmental samples (soil, manure, water) were collected from 17 farms. Target bacteria were isolated and confirmed using selective agar, biochemical tests, and PCR, and tested against 12 antibiotics using the Kirby\u2013Bauer disk diffusion test. Multiple Antibiotic Resistance Index (MARI) and multidrug resistance (MDR) profiles were summarized. A complementary farmer survey of 26 farmers captured veterinary access, antibiotic use, manure handling, record keeping, and awareness of antimicrobial resistance. Results: Prevalence was highest for Enterococcus spp. (41.8%), followed by E. coli (23.5%) and Klebsiella spp. (12.4%). Seasonal variation was significant for E. coli and Enterococcus (p &lt; 0.05). Winter manure yielded highest detection of E. coli (55.6%) and Enterococcus (53.8%), whereas Klebsiella peaked in Fall soil (19.1%). Resistance patterns varied across species, with Enterococcus showing consistent resistance to all three. E. coli frequently resisted erythromycin, ampicillin, and azithromycin; and Klebsiella commonly resisted erythromycin, ampicillin, and cefotaxime, though some of these reflect intrinsic resistance rather than acquired clinical resistance. MARI values were 0.92 in manure and soil, identifying them as high-risk reservoirs. We identified 29 distinct MDR pattern. Bipartite network visualization highlighted \u201cresistance hubs\u201d around erythromycin, ampicillin, and vancomycin, particularly in Enterococcus. In our study, 76.9% of farmers consulted veterinarians before antibiotic use, 57.7% kept written antibiotic records, and 65.4% were aware of AMR as a public health issue. Small-scale cattle farms are potential reservoirs of multidrug resistant commensal bacteria. Conclusions: These findings provide an evidence-based foundation to guide targeted antimicrobial stewardship and promote sustainable management practices in small-scale food animal farms.<\/jats:p>","DOI":"10.3390\/antibiotics15020217","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T09:22:46Z","timestamp":1771320166000},"page":"217","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Susceptibility Profile and Multiple Antibiotics Resistance of Escherichia\u00a0coli, Klebsiella spp., and Enterococci from Small-Scale Cattle Farms in Tennessee"],"prefix":"10.3390","volume":"15","author":[{"given":"Goodness","family":"Olakanmi","sequence":"first","affiliation":[{"name":"Department of Food and Animal Sciences, Tennessee State University, 3500 John A. Merritt Boulevard, Nashville, TN 37209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maureen","family":"Nzomo","sequence":"additional","affiliation":[{"name":"Department of Food and Animal Sciences, Tennessee State University, 3500 John A. Merritt Boulevard, Nashville, TN 37209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5900-9972","authenticated-orcid":false,"given":"Bharat","family":"Pokharel","sequence":"additional","affiliation":[{"name":"Department of Food and Animal Sciences, Tennessee State University, 3500 John A. Merritt Boulevard, Nashville, TN 37209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah","family":"Mafiz","sequence":"additional","affiliation":[{"name":"Department of Food and Animal Sciences, Tennessee State University, 3500 John A. Merritt Boulevard, Nashville, TN 37209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnes","family":"Kilonzo-Nthenge","sequence":"additional","affiliation":[{"name":"Department of Food and Animal Sciences, Tennessee State University, 3500 John A. Merritt Boulevard, Nashville, TN 37209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.4103\/2045-080X.186181","article-title":"Tackling drug-resistant infections globally: Final report and recommendations","volume":"7","year":"2016","journal-title":"Arch. Pharm. Pract."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Manyi-Loh, C., Mamphweli, S., Meyer, E., and Okoh, A. (2018). Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. 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