{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T05:59:15Z","timestamp":1774159155138,"version":"3.50.1"},"reference-count":27,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: Drug susceptibility testing in Mycobacterium species typically requires prolonged incubation periods during which the chemical integrity of antibiotics may not be maintained, potentially compromising the reliability and accuracy of minimum inhibitory concentration (MIC) determinations. Objectives: This study evaluated the in vitro stability of several antibiotics, including recently introduced agents (bedaquiline [BDQ], pretomanid, delamanid and clofazimine) used for treating multidrug-resistant mycobacteriosis (linezolid and moxifloxacin), and those commonly included in combination regimens (rifampicin, isoniazid, ethambutol and clarithromycin). Methods: Antibiotics were pre-incubated at 37 \u00b0C before MIC determination and those exhibiting two or more dilutions in MIC were further tested in combination with ascorbic acid (AA) and pyruvate (P). Results: All antibiotics demonstrated stability except BDQ, which showed significant MIC variation after pre-incubation, which was prevented when BDQ was combined with AA and P. Conclusions: These findings suggest that the combined use of AA and P may serve as an effective stabilizing strategy for BDQ during MIC determination.<\/jats:p>","DOI":"10.3390\/antibiotics15030316","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T09:44:41Z","timestamp":1773999881000},"page":"316","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced In Vitro Stability of Bedaquiline with Ascorbic Acid and Pyruvate During Long-Term Incubation in Mycobacterium Species"],"prefix":"10.3390","volume":"15","author":[{"given":"Sara","family":"Batista","sequence":"first","affiliation":[{"name":"Departament de Fonaments Cl\u00ednics, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, c\/Casanova 143, 08036 Barcelona, Spain"},{"name":"Fundaci\u00f3 de Recerca Cl\u00ednic Barcelona-Institut d\u2019Investigacions Biom\u00e8diques August\u00ed Pi i Sunyer (FRCB-IDIBAPS), 08036 Barcelona, Spain"},{"name":"Servei de Microbiologia, CDB, Hospital Cl\u00ednic de Barcelona, c\/Villarroel 170, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi","family":"Lamata","sequence":"additional","affiliation":[{"name":"LIPPSO, Departament de Qu\u00edmica, Institut de Tecnologia Agroaliment\u00e0ria, Universitat de Girona, Maria Aur\u00e8lia Capmany 69, 17003 Girona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9792-6106","authenticated-orcid":false,"given":"Lidia","family":"Feliu","sequence":"additional","affiliation":[{"name":"LIPPSO, Departament de Qu\u00edmica, Institut de Tecnologia Agroaliment\u00e0ria, Universitat de Girona, Maria Aur\u00e8lia Capmany 69, 17003 Girona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4988-4970","authenticated-orcid":false,"given":"Marta","family":"Planas","sequence":"additional","affiliation":[{"name":"LIPPSO, Departament de Qu\u00edmica, Institut de Tecnologia Agroaliment\u00e0ria, Universitat de Girona, Maria Aur\u00e8lia Capmany 69, 17003 Girona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana","family":"Fernandez-Pittol","sequence":"additional","affiliation":[{"name":"Departament de Fonaments Cl\u00ednics, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, c\/Casanova 143, 08036 Barcelona, Spain"},{"name":"Servei de Microbiologia, CDB, Hospital Cl\u00ednic de Barcelona, c\/Villarroel 170, 08036 Barcelona, Spain"},{"name":"ISGlobal Barcelona, Institute for Global Health, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Martinez","sequence":"additional","affiliation":[{"name":"Servei de Microbiologia, CDB, Hospital Cl\u00ednic de Barcelona, c\/Villarroel 170, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena","family":"San Nicol\u00e1s","sequence":"additional","affiliation":[{"name":"Servei de Microbiologia, CDB, Hospital Cl\u00ednic de Barcelona, c\/Villarroel 170, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9241-7051","authenticated-orcid":false,"given":"Griselda","family":"Tud\u00f3","sequence":"additional","affiliation":[{"name":"Departament de Fonaments Cl\u00ednics, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, c\/Casanova 143, 08036 Barcelona, Spain"},{"name":"Servei de Microbiologia, CDB, Hospital Cl\u00ednic de Barcelona, c\/Villarroel 170, 08036 Barcelona, Spain"},{"name":"ISGlobal Barcelona, Institute for Global Health, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7248-6706","authenticated-orcid":false,"given":"Julian","family":"Gonzalez-Martin","sequence":"additional","affiliation":[{"name":"Departament de Fonaments Cl\u00ednics, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, c\/Casanova 143, 08036 Barcelona, Spain"},{"name":"Servei de Microbiologia, CDB, Hospital Cl\u00ednic de Barcelona, c\/Villarroel 170, 08036 Barcelona, Spain"},{"name":"ISGlobal Barcelona, Institute for Global Health, 08036 Barcelona, Spain"},{"name":"CIBER of Infectious Diseases (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,20]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2025). 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