{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:04:01Z","timestamp":1790197441742,"version":"4.1.0"},"reference-count":122,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives:\u00a0Escherichia coli is a ubiquitous commensal organism in humans, animals, and the environment, but certain strains harbour virulence and antimicrobial resistance (AMR) determinants that can cause significant disease. Food-producing animals, including dairy cattle, may act as reservoirs for AMR E. coli, and raw milk and raw milk products can serve as potential exposure pathways to humans. However, data on the prevalence and genomic characteristics of AMR E. coli in raw milk in Ireland are limited. This study aimed to describe the occurrence of commensal and clinically relevant AMR E. coli in raw milk and raw milk dairy products in Ireland and to characterise their antimicrobial susceptibility and genetic characteristics. Methods: A total of 139 raw milk and raw milk dairy product samples were collected and analysed for commensal E. coli and fluoroquinolone-resistant, extended-spectrum \u03b2-lactamase (ESBL)\/AmpC \u03b2-lactamase and carbapenemase-producing E. coli. AMR patterns were determined in line with EU surveillance guidelines based on the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines which use minimum inhibitory concentration (MIC) breakpoints. Whole genome sequencing (WGS) was conducted on selected isolates to identify AMR genes (ARG), virulence factors, plasmid replicons, efflux pump, disinfectant resistance genes, multi-locus sequence types (MLSTs) and phylogenetic diversity. Results: A total of forty-seven E. coli isolates were recovered (33.8% isolation rate). Thirteen isolates exhibited resistance to between two and nine antimicrobials, with twelve classified as multidrug resistant (MDR). The highest resistance frequencies were to ampicillin, sulfamethoxazole, trimethoprim and tetracycline. Four fluoroquinolone-resistant isolates, one ESBL producer (blaCTX-M-3), and one carrying a AmpC promoter mutation were identified; no carbapenemase producers were detected. WGS revealed diverse sequence types, multiple virulence determinants, plasmid replicons, intrinsic efflux pump genes, and limited presence of the disinfectant resistance gene qacE\u03941. Conclusions: Raw milk and raw milk dairy products in Ireland can harbour AMR E. coli, including MDR and potentially pathogenic strains, highlighting the need for ongoing surveillance within the dairy supply chain.<\/jats:p>","DOI":"10.3390\/antibiotics15040326","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T12:58:59Z","timestamp":1774357139000},"page":"326","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phenotypic and Genomic Profiling of Escherichia coli from Irish Raw Milk and Raw Milk Products: A Baseline Study"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1948-3703","authenticated-orcid":false,"given":"Deirdre M.","family":"Prendergast","sequence":"first","affiliation":[{"name":"Department of Agriculture, Food and the Marine (DAFM) Laboratories, Backweston Laboratory Campus, Celbridge, W23 X3PH Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian","family":"Teeling","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and the Marine (DAFM) Laboratories, Backweston Laboratory Campus, Celbridge, W23 X3PH Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and the Marine (DAFM) Laboratories, Backweston Laboratory Campus, Celbridge, W23 X3PH Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saibh","family":"Healy","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and the Marine (DAFM) Laboratories, Backweston Laboratory Campus, Celbridge, W23 X3PH Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"Madigan","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and the Marine (DAFM) Laboratories, Backweston Laboratory Campus, Celbridge, W23 X3PH Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sin\u00e9ad","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and the Marine (DAFM) Laboratories, Backweston Laboratory Campus, Celbridge, W23 X3PH Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5613-441X","authenticated-orcid":false,"given":"Amalia","family":"Naranjo-Lucena","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and the Marine (DAFM) Laboratories, Backweston Laboratory Campus, Celbridge, W23 X3PH Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montserrat","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and the Marine (DAFM) Laboratories, Backweston Laboratory Campus, Celbridge, W23 X3PH Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/S1286-4579(03)00049-2","article-title":"Medical and economic impact of extraintestinal infections due to Escherichia coli: Focus on an increasingly important endemic problem","volume":"5","author":"Russo","year":"2003","journal-title":"Microbes Infect."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Pakbin, B., Br\u00fcck, W.M., and Rossen, J.W.A. 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