{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T03:04:37Z","timestamp":1780628677242,"version":"3.54.1"},"reference-count":33,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Catheter-associated urinary tract infections (CAUTIs) are one of the most common healthcare-related morbidities. Also, severe clinical outcomes derived from CAUTIs demand an urgent need for the development of novel antimicrobial catheter materials. Since CAUTIs are primarily driven by a wide range of microorganisms causing biofilm formation on the surfaces of catheters, evaluating the effectiveness of innovative antimicrobial materials against a broad spectrum of known uropathogens is warranted. We aim (1) to demonstrate the ability of incorporated organo-selenium versus that of an FDA-cleared antimicrobial catheter and (2) to show that the results of the study are consistent against the most common microorganisms causing urinary tract infections in humans. Methods: Based on encouraging preliminary studies, three percent of organo-selenium (weight-based), as a novel antimicrobial catheter material, was incorporated into thermoplastic polyurethane (TPU). This was compared in vitro with plain polyurethane catheters and the SILQ ClearTract catheter with a zwitterionic coating (FDA-cleared for its antimicrobial properties in December 2022). The antimicrobial activity was studied against Candida albicans, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus, MRSA, and Staphylococcus epidermidis by assessment of colony-forming unit counts along with visual confirmation using Scanning Electron Microscopy (SEM). Results: Plain polyurethane catheters (control group) showed 7- to 8-log of in vitro growth for all tested microbes, whereas the antimicrobial zwitterionic SILQ ClearTract catheters still showed 6- to 7-log of microbial growth. In contrast, organo-selenium-incorporated catheters demonstrated no detectable in vitro growth for all tested microbes (C. albicans, E. coli, K. pneumoniae, P. mirabilis, P. aeruginosa, S. aureus, MRSA, and S. epidermidis). SEM analysis also validated the findings. Conclusions: Potentially non-leaching organo-selenium, as a novel urinary catheter material, significantly inhibited microbial attachment, growth, and biofilm formation across a wide spectrum of common uropathogenic organisms compared to a zwitterionic catheter, providing a strong foundation for further detailed in vivo and clinical studies.<\/jats:p>","DOI":"10.3390\/antibiotics15060574","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T01:23:21Z","timestamp":1780622601000},"page":"574","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Comprehensive In Vitro Assessment of Broad-Spectrum Antimicrobial Efficacy of Organo-Selenium-Incorporated Urinary Catheter in Comparison to a Zwitterionic Surface Catheter"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3902-307X","authenticated-orcid":false,"given":"Harry","family":"May","sequence":"first","affiliation":[{"name":"School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9950-8623","authenticated-orcid":false,"given":"Md Abid","family":"Afridi","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3438-5829","authenticated-orcid":false,"given":"Phat L.","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7281-1407","authenticated-orcid":false,"given":"Hannah","family":"Seo","sequence":"additional","affiliation":[{"name":"School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8980-8002","authenticated-orcid":false,"given":"Eric","family":"Tran","sequence":"additional","affiliation":[{"name":"School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9646-5483","authenticated-orcid":false,"given":"Ted W.","family":"Reid","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1963-2334","authenticated-orcid":false,"given":"Werner T. W.","family":"de Riese","sequence":"additional","affiliation":[{"name":"Department of Urology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1017\/ice.2023.137","article-title":"Strategies to prevent catheter-associated urinary tract infections in acute-care hospitals: 2022 Update","volume":"44","author":"Patel","year":"2023","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"552","DOI":"10.12788\/jhm.3292","article-title":"Catheter-Associated Urinary Tract Infections in Adults: Diagnosis, Treatment, and Prevention","volume":"15","author":"Clarke","year":"2020","journal-title":"J. Hosp. Med."},{"key":"ref_3","first-page":"109","article-title":"Catheter-Associated Urinary Tract Infections: Current Challenges and Future Prospects","volume":"14","author":"Werneburg","year":"2022","journal-title":"Res. Rep. Urol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"S65","DOI":"10.1016\/0002-9343(91)90345-X","article-title":"Catheter-associated urinary tract infections: Epidemiology, pathogenesis, and prevention","volume":"91","author":"Stamm","year":"1991","journal-title":"Am. J. Med."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Tegegne, D.T., Abbott, I.J., and Po\u017aniak, B. (2025). Catheter-Associated Urinary Tract Infections: Understanding the Interplay Between Bacterial Biofilm and Antimicrobial Resistance. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26189193"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1086\/651091","article-title":"Guideline for Prevention of Catheter-Associated Urinary Tract Infections 2009","volume":"31","author":"Gould","year":"2010","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Tran, P.L., Presson, C.L., Kashem, M.N.H., Li, W., Reid, T.W., and De Riese, W.T.W. (2024). Complete Growth Inhibition of Pseudomonas aeruginosa by Organo-Selenium-Incorporated Urinary Catheter Material. Antibiotics, 13.","DOI":"10.3390\/antibiotics13080736"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1097\/01.ico.0000224636.57062.90","article-title":"Prevention of Bacterial Colonization of Contact Lenses with Covalently Attached Selenium and Effects on the Rabbit Cornea","volume":"25","author":"Mathews","year":"2006","journal-title":"Cornea"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1128\/AAC.05680-11","article-title":"An Organoselenium Compound Inhibits Staphylococcus aureus Biofilms on Hemodialysis Catheters In Vivo","volume":"56","author":"Tran","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.prosdent.2021.04.015","article-title":"Determining growth inhibition of Candida albicans biofilm on denture materials after application of an organoselenium-containing dental sealant","volume":"129","author":"AlMojel","year":"2023","journal-title":"J. Prosthet. Dent."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/s11255-022-03422-y","article-title":"Efficacy of organo-selenium-incorporated urinary catheter tubing for in vitro growth inhibition of E. coli, K. pneumoniae, P. aeruginosa, and H. influenzae","volume":"55","author":"Jacobo","year":"2022","journal-title":"Int. Urol. Nephrol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2200254","DOI":"10.1002\/adma.202200254","article-title":"A Readily Scalable, Clinically Demonstrated, Antibiofouling Zwitterionic Surface Treatment for Implantable Medical Devices","volume":"34","author":"McVerry","year":"2022","journal-title":"Adv. Mater."},{"key":"ref_13","first-page":"48","article-title":"Uropathogens and their antimicrobial resistance patterns: Relationship with urinary tract infections","volume":"13","author":"Ahmed","year":"2019","journal-title":"Int. J. Health Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"ofw281","DOI":"10.1093\/ofid\/ofw281","article-title":"The Increase in Hospitalizations for Urinary Tract Infections and the Associated Costs in the United States, 1998\u20132011","volume":"4","author":"Simmering","year":"2017","journal-title":"Open Forum Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"S831","DOI":"10.1093\/ofid\/ofaa439.1875","article-title":"1697. The Burden of Multidrug-Resistant Urinary Tract Infections","volume":"7","author":"Hammami","year":"2020","journal-title":"Open Forum Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"125","DOI":"10.7326\/0003-4819-137-2-200207160-00012","article-title":"Indwelling Urinary Catheters: A One-Point Restraint?","volume":"137","author":"Saint","year":"2002","journal-title":"Ann. Intern. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1086\/501908","article-title":"The Chronic Indwelling Catheter and Urinary Infection in Long-Term\u2013Care Facility Residents","volume":"22","author":"Smith","year":"2001","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.gerinurse.2014.04.010","article-title":"Evidenced based review of recommendations addressing the frequency of changing long-term indwelling urinary catheters in older adults","volume":"35","author":"Palka","year":"2014","journal-title":"Geriatr. Nurs."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1016\/S0140-6736(12)61380-4","article-title":"Antimicrobial catheters for reduction of symptomatic urinary tract infection in adults requiring short-term catheterisation in hospital: A multicentre randomised controlled trial","volume":"380","author":"Pickard","year":"2012","journal-title":"Lancet"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3310\/hta16470","article-title":"Types of urethral catheter for reducing symptomatic urinary tract infections in hospitalised adults requiring short-term catheterisation: Multicentre randomised controlled trial and economic evaluation of antimicrobial- and antiseptic-impregnated urethral catheters (the CATHETER trial)","volume":"16","author":"Pickard","year":"2012","journal-title":"Health Technol. Assess."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"051506","DOI":"10.1063\/5.0195165","article-title":"Recent developments in preventing catheter-related infections based on biofilms: A comprehensive review","volume":"18","author":"So","year":"2024","journal-title":"Biomicrofluidics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1089\/ars.2010.3275","article-title":"Selenium in Human Health and Disease","volume":"14","author":"Bao","year":"2011","journal-title":"Antioxid. Redox Signal."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s00240-009-0176-6","article-title":"A study of the structure of the crystalline bacterial biofilms that can encrust and block silver Foley catheters","volume":"37","author":"Morgan","year":"2009","journal-title":"Urol. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1016\/j.juro.2010.07.036","article-title":"Silver or nitrofurazone impregnation of urinary catheters has a minimal effect on uropathogen adherence","volume":"184","author":"Desai","year":"2010","journal-title":"J. Urol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1086\/499998","article-title":"A Prospective Trial of a Novel, Silicone-Based, Silver-Coated Foley Catheter for the Prevention of Nosocomial Urinary Tract Infections","volume":"27","author":"Srinivasan","year":"2006","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Gauhar, V., Castellani, D., Teoh, J.Y.C., Nedbal, C., Chiacchio, G., Gabrielson, A.T., Heldwein, F.L., Wroclawski, M.L., de la Rosette, J., and Donalisio da Silva, R. (2022). Catheter-Associated Urinary Infections and Consequences of Using Coated versus Non-Coated Urethral Catheters-Outcomes of a Systematic Review and Meta-Analysis of Randomized Trials. J. Clin. Med., 11.","DOI":"10.3390\/jcm11154463"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/2047-2994-3-23","article-title":"Catheter associated urinary tract infections","volume":"3","author":"Nicolle","year":"2014","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1017\/ice.2016.174","article-title":"Antimicrobial-Resistant Pathogens Associated with Healthcare-Associated Infections: Summary of Data Reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2011-2014","volume":"37","author":"Weiner","year":"2016","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1524","DOI":"10.1093\/infdis\/jit663","article-title":"Increased incidence of urolithiasis and bacteremia during Proteus mirabilis and Providencia stuartii coinfection due to synergistic induction of urease activity","volume":"209","author":"Armbruster","year":"2014","journal-title":"J. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.desal.2013.10.007","article-title":"Evaluation of polymerized organo-selenium feed spacers to inhibit S. aureus and E. coli biofilm development in reverse osmosis systems","volume":"331","author":"Vercellino","year":"2013","journal-title":"Desalination"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"E8721","DOI":"10.1073\/pnas.1707572114","article-title":"Catheterization alters bladder ecology to potentiate Staphylococcus aureus infection of the urinary tract","volume":"114","author":"Walker","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4613","DOI":"10.2147\/IJN.S197737","article-title":"Selenium nanoparticles as anti-infective implant coatings for trauma orthopedics against methicillin-resistant Staphylococcus aureus and epidermidis: In vitro and in vivo assessment","volume":"14","author":"Tran","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Navarro, S., Sherman, E., Colmer-Hamood, J.A., Nelius, T., Myntti, M., and Hamood, A.N. (2022). Urinary Catheters Coated with a Novel Biofilm Preventative Agent Inhibit Biofilm Development by Diverse Bacterial Uropathogens. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111514"}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/15\/6\/574\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T02:21:04Z","timestamp":1780626064000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/15\/6\/574"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,4]]},"references-count":33,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["antibiotics15060574"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics15060574","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,4]]}}}