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Kaszab) of the Hungarian University of Agriculture and Life Sciences"},{"id":[{"id":"https:\/\/ror.org\/01394d192","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003825","name":"J\u00e1nos Bolyai Research Grant of the Hungarian Academy of Sciences","doi-asserted-by":"publisher","award":["BO\/00067\/24\/4"],"award-info":[{"award-number":["BO\/00067\/24\/4"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]},{"award":["BO\/00067\/24\/4"],"award-info":[{"award-number":["BO\/00067\/24\/4"]}],"id":[{"id":"https:\/\/ror.org\/02ks8qq67","id-type":"ROR","asserted-by":"publisher"}]},{"name":"EK\u00d6P-25-D\/MATE-20 university research Scholarship Programme of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: The global emergence of antibiotic resistance highlights the urgent need for novel therapeutic strategies, including adjuvants and potentiating compounds, against multidrug-resistant bacteria. Pseudomonas aeruginosa is classified by the World Health Organization (WHO) as a critical priority pathogen due to its high resistance potential and its ability to cause severe nosocomial infections. Beauvericin (BEA), a frequently detected mycotoxin, has been reported to exhibit various bioactive properties, including potential antibacterial and potentiating effects. Methods: The interaction between BEA and a last-resort antibiotic, colistin (COL), was evaluated in seven multidrug-resistant P. aeruginosa isolates using a microplate-based growth assay after preliminary MIC tests. Results: BEA at non-inhibitory concentrations (2.5\u201310 \u00b5g\/mL) significantly enhanced the antibacterial activity of COL (1 and 2 \u00b5g\/mL) in six out of seven isolates, resulting in a marked reduction in residual bacterial growth. Conclusions: BEA exhibited no measurable antibacterial activity at the concentrations used in the combination experiments but acted as a strain-dependent potentiator of colistin activity against multidrug-resistant P. aeruginosa. The observed enhancement of colistin-mediated growth inhibition supports the potential of BEA as an antibiotic adjuvant at clinically relevant colistin concentrations and provides a basis for further mechanistic investigation.<\/jats:p>","DOI":"10.3390\/antibiotics15070631","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:37:17Z","timestamp":1782279437000},"page":"631","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Potentiating Effect of Beauvericin on Colistin, a Last Resort Antibiotic in Multidrug-Resistant Pseudomonas aeruginosa Strains"],"prefix":"10.3390","volume":"15","author":[{"given":"\u00c1kos","family":"Suhajda","sequence":"first","affiliation":[{"name":"Department of Molecular Ecology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences (MATE), P\u00e1ter K\u00e1roly utca 1, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1ty\u00e1s","family":"Cserh\u00e1ti","sequence":"additional","affiliation":[{"name":"Department of Molecular Ecology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences (MATE), P\u00e1ter K\u00e1roly utca 1, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1787-5359","authenticated-orcid":false,"given":"Judit","family":"H\u00e1hn","sequence":"additional","affiliation":[{"name":"Department of Environmental Safety, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences (MATE), P\u00e1ter K\u00e1roly utca 1, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5917-0464","authenticated-orcid":false,"given":"Edit","family":"Kaszab","sequence":"additional","affiliation":[{"name":"Department of Environmental Safety, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences (MATE), P\u00e1ter K\u00e1roly utca 1, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda","family":"F\u00f3zer","sequence":"additional","affiliation":[{"name":"Department of Environmental Safety, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences (MATE), P\u00e1ter K\u00e1roly utca 1, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7897-5778","authenticated-orcid":false,"given":"Csilla","family":"Krifaton","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicity, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences (MATE), P\u00e1ter K\u00e1roly utca 1, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8954-9085","authenticated-orcid":false,"given":"Ren\u00e1ta","family":"\u00c1brah\u00e1m","sequence":"additional","affiliation":[{"name":"Department of Molecular Ecology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences (MATE), P\u00e1ter K\u00e1roly utca 1, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal\u00e1zs","family":"Kriszt","sequence":"additional","affiliation":[{"name":"Department of Environmental Safety, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences (MATE), P\u00e1ter K\u00e1roly utca 1, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1007\/s00203-025-04340-0","article-title":"Understanding current challenges of antibiotic resistance to find new solutions for 2050: Scientific, medical and political issues","volume":"207","author":"Georges","year":"2025","journal-title":"Arch. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1080\/1040841X.2024.2313024","article-title":"Antibiotic resistance: A global crisis, problems and solutions","volume":"50","author":"Aggarwal","year":"2024","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.imj.2023.05.003","article-title":"Pseudomonas aeruginosa: Infections and novel approaches to treatment\u2014\u201cKnowing the enemy\u201d","volume":"2","author":"Sathe","year":"2023","journal-title":"Infect. Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Frem, J.A., Doumat, G., Kazma, J., Gharamti, A., Kanj, S.S., Abou Fayad, A.G., Matar, G.M., and Kanafani, Z.A. (2023). Clinical predictors of mortality in patients with Pseudomonas aeruginosa infection. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0282276"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Poole, K. (2011). Pseudomonas aeruginosa: Resistance to the max. Front. Microbiol., 2.","DOI":"10.3389\/fmicb.2011.00065"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1080\/09603123.2019.1691719","article-title":"Groundwater, soil and compost as possible sources of virulent and antibiotic-resistant Pseudomonas aeruginosa","volume":"31","author":"Kaszab","year":"2021","journal-title":"Int. J. Environ. Health Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1111\/apm.13010","article-title":"The environmental occurrence of Pseudomonas aeruginosa","volume":"128","author":"Crone","year":"2020","journal-title":"APMIS"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.arcmed.2004.02.005","article-title":"Relationship between clinical and environmental isolates of Pseudomonas aeruginosa in a hospital setting","volume":"35","author":"Ruiz","year":"2004","journal-title":"Arch. Med. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e29987","DOI":"10.1016\/j.heliyon.2024.e30215","article-title":"Comparing antibiotic resistance and virulence profiles of Enterococcus faecium, Klebsiella pneumoniae, and Pseudomonas aeruginosa from environmental and clinical settings","volume":"10","author":"Denissen","year":"2024","journal-title":"Heliyon"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"18258","DOI":"10.1038\/s41598-022-23117-9","article-title":"Glyphosate and glyphosate-based herbicides induce phenotypic imipenem resistance in Pseudomonas aeruginosa","volume":"12","author":"Kriszt","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.tim.2023.11.005","article-title":"Pseudomonas\u00a0aeruginosa","volume":"32","author":"Krell","year":"2024","journal-title":"Trends Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"290","DOI":"10.2471\/BLT.22.288614","article-title":"The WHO AWaRe antibiotic book and prevention of antimicrobial resistance","volume":"101","author":"Zanichelli","year":"2023","journal-title":"Bull. World Health Organ."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Cervoni, M., Ferriero, A.M., Lo Sciuto, A., Guidi, F., Babi\u0107 Jordamovi\u0107, N., Piazza, S., Jousson, O., Esposito, A., and Imperi, F. (2025). The genetic background and culture medium only marginally affect the in vitro evolution of Pseudomonas aeruginosa toward colistin resistance. Antibiotics, 14.","DOI":"10.3390\/antibiotics14060601"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1185\/03007995.2015.1018989","article-title":"Colistin, mechanisms and prevalence of resistance","volume":"31","author":"Bialvaei","year":"2015","journal-title":"Curr. Med. Res. Opin."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1080\/17425255.2017.1230200","article-title":"Colistin: Still a lifesaver for the 21st century?","volume":"13","author":"Karaiskos","year":"2017","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Landersdorfer, C.B., and Nation, R.L. (2015). Colistin: How should it be dosed for the critically ill?. Seminars in Respiratory and Critical Care Medicine, Thieme.","DOI":"10.1055\/s-0034-1398390"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"149280","DOI":"10.1016\/j.scitotenv.2021.149280","article-title":"Updates on the global dissemination of colistin-resistant Escherichia coli: An emerging threat","volume":"799","author":"Zhang","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"555985","DOI":"10.19080\/AIBM.2024.18.555985","article-title":"Antibiotics production from fungi","volume":"18","author":"Gemechu","year":"2024","journal-title":"Adv. Biotechnol. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.3390\/molecules17032367","article-title":"Beauvericin, a bioactive compound produced by fungi: A short review","volume":"17","author":"Wang","year":"2012","journal-title":"Molecules"},{"key":"ref_20","unstructured":"DSM-Firmenich (2026, March 11). World Mycotoxin Survey 2024: The Global Threat. Available online: https:\/\/www.dsm-firmenich.com\/anh\/news\/downloads\/whitepapers-and-reports\/dsm-firmenich-world-mycotoxin-survey-january-to-december-2024.html."},{"key":"ref_21","unstructured":"DSM-Firmenich (2026, March 11). World Mycotoxin Survey 2025: The Global Threat. Available online: https:\/\/www.dsm-firmenich.com\/anh\/products-and-services\/tools\/mycotoxin-contamination\/mycotoxin-survey\/mycotoxin-survey-q4-2025.html."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Contaminants in the Food Chain (CONTAM), Knutsen, H.K., \u00c5kesson, A., Bampidis, V., Bodin, L., Chipman, J.K., Degen, G., Hern\u00e1ndez-Jerez, A., Hofer, T., and Hogstrand, C. (2024). Genotoxicity of beauvericin. EFSA J., 22, e9031.","DOI":"10.2903\/j.efsa.2024.9031"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wu, Q., Patocka, J., Nepovimova, E., and Kuca, K. (2018). Beauvericin: Synthesis and bioactivity. Front. Pharmacol., 9.","DOI":"10.3389\/fphar.2018.01338"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e0136823","DOI":"10.1128\/aac.01368-23","article-title":"ABC transporter inhibition by beauvericin","volume":"68","author":"Thoumi","year":"2024","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_25","first-page":"7468","article-title":"Beauvericin potentiates azole activity","volume":"60","author":"Tebung","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.synbio.2016.10.001","article-title":"Beauvericin counteracts multidrug-resistant Candida albicans","volume":"1","author":"Tong","year":"2016","journal-title":"Synth. Syst. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4606","DOI":"10.1073\/pnas.0609370104","article-title":"High-throughput synergy screening identifies microbial metabolites as combination agents for the treatment of fungal infections","volume":"104","author":"Zhang","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"107713","DOI":"10.1016\/j.toxicon.2024.107713","article-title":"Interaction of beauvericin in combination with antibiotics against MRSA and Salmonella typhimurium","volume":"243","author":"Bonilla","year":"2024","journal-title":"Toxicon"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.toxicon.2010.03.022","article-title":"Antibacterial effect of beauvericin","volume":"56","author":"Meca","year":"2010","journal-title":"Toxicon"},{"key":"ref_30","first-page":"811","article-title":"Beauvericin from Fusarium redolens","volume":"5","author":"Xu","year":"2010","journal-title":"Nat. Prod. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Olleik, H., Nicoletti, C., Lafond, M., Courvoisier-Dezord, E., Xue, P., Hijazi, A., Baydoun, E., Perrier, J., and Maresca, M. (2019). Comparative structure\u2013activity analysis of enniatins and beauvericin. Toxins, 11.","DOI":"10.3390\/toxins11090514"},{"key":"ref_32","first-page":"1084670","article-title":"Isolation of beauvericin from Fusarium oxysporum","volume":"2016","author":"Zhang","year":"2016","journal-title":"Biomed. Res. Int."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"107534","DOI":"10.1016\/j.toxicon.2023.107534","article-title":"Toxicity of beauvericin and enniatins","volume":"239","author":"Hasuda","year":"2024","journal-title":"Toxicon"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Andrade, F.F., Silva, D., Rodrigues, A., and Pina-Vaz, C. (2020). Colistin mechanism and resistance. Microorganisms, 8.","DOI":"10.3390\/microorganisms8111716"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s00248-009-9551-7","article-title":"Multidrug-resistant Pseudomonas aeruginosa on hydrocarbon-contaminated sites","volume":"59","author":"Kaszab","year":"2010","journal-title":"Microb. Ecol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kaszab, E., Jiang, D., Szab\u00f3, I., Kriszt, B., Urb\u00e1nyi, B., Szoboszlay, S., Seb\u0151k, R., Bock, I., and Csenki-Bakos, Z. (2023). Virulence of environmental Pseudomonas aeruginosa in zebrafish model. Antibiotics, 12.","DOI":"10.3390\/antibiotics12121740"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Standard definitions for MDR\/XDR\/PDR bacteria","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/S0045-6535(01)00209-0","article-title":"Time-dependent toxicity in the long-term inhibition assay with Vibrio fischeri","volume":"46","author":"Froehner","year":"2002","journal-title":"Chemosphere"}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/15\/7\/631\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T04:21:24Z","timestamp":1782706884000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/15\/7\/631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,23]]},"references-count":38,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["antibiotics15070631"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics15070631","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,23]]}}}