{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:33:13Z","timestamp":1785407593902,"version":"3.56.0"},"reference-count":28,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008836","name":"Gaziantep University","doi-asserted-by":"publisher","award":["FEF.DKP.26.10."],"award-info":[{"award-number":["FEF.DKP.26.10."]}],"id":[{"id":"10.13039\/501100008836","id-type":"DOI","asserted-by":"publisher"}]},{"award":["FEF.DKP.26.10."],"award-info":[{"award-number":["FEF.DKP.26.10."]}],"id":[{"id":"https:\/\/ror.org\/020vvc407","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background:\u00a0Candida auris has emerged globally as a multidrug-resistant fungal pathogen responsible for healthcare-associated outbreaks and invasive infections. Whole-genome sequencing studies have demonstrated the existence of genetically distinct clades that differ in geographical distribution, antifungal resistance patterns, virulence traits, and outbreak potential. Objectives: This study aimed to evaluate the performance of the ClaID clade identification PCR system among clinical Candida auris isolates collected in \u0130stanbul, T\u00fcrkiye, and to investigate the clade-associated molecular profiles of circulating isolates. Methods: Forty-four clinical C. auris isolates were analysed using the auris universal sequence (AUS) assay and clade-specific sequence assays (CSS1\u2013CSS5). PCR amplification results were interpreted according to the ClaID framework. Results: AUS amplification was detected in 41\/44 isolates (93.2%). CSS1 amplification was observed in 39\/44 isolates (88.6%), indicating a predominance of Clade I-associated molecular profiles within this regional \u0130stanbul isolate collection. No amplification was detected using CSS2, CSS3, CSS4, or CSS5 assays. Three isolates were AUS-negative and five isolates did not yield CSS1 amplification despite repeated testing. Conclusions: The findings suggest that the majority of analyzed clinical isolates from \u0130stanbul exhibited Clade I-associated molecular profiles rather than definitive WGS-confirmed clade assignments. This study provides one of the first field evaluations of the ClaID system in a Turkish clinical isolate collection and contributes regional molecular epidemiological data regarding PCR-based clade-associated profiles of C. auris in T\u00fcrkiye.<\/jats:p>","DOI":"10.3390\/antibiotics15070692","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T06:49:36Z","timestamp":1784184576000},"page":"692","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Field Evaluation of the ClaID PCR System Reveals Predominance of Clade I-Associated Molecular Profiles Among Clinical Candida auris Isolates Recovered in \u0130stanbul, T\u00fcrkiye"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4705-5757","authenticated-orcid":false,"given":"Feray Ferda","family":"\u015eenol","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Elaz\u0131\u011f Fethi Sekin City Hospital, Elaz\u0131\u011f 23280, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z\u00fclal","family":"A\u015f\u00e7\u0131 Toraman","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, F\u0131rat University, Elaz\u0131\u011f 23119, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U\u011fur","family":"Vural","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science and Letters, Gaziantep University, Gaziantep 27310, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynep","family":"\u00c7elik","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science and Letters, Gaziantep University, Gaziantep 27310, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1297-944X","authenticated-orcid":false,"given":"Y\u00fcksel","family":"Akkaya","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Hamidiye Faculty of Medicine, University of Health Sciences, \u0130stanbul 34668, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"\u00c7ilk\u0131z","sequence":"additional","affiliation":[{"name":"Ankara Poultry Research Institute, Ministry of Agriculture and Forestry, Ankara 06170, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9782-5671","authenticated-orcid":false,"given":"Beg\u00fcm","family":"Nal\u00e7a Erdin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Dar\u0131ca State Hospital, Kocaeli 41700, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4337-8236","authenticated-orcid":false,"given":"Sevda","family":"K\u0131rba\u011f","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, F\u0131rat University, Elaz\u0131\u011f 23119, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0272-5131","authenticated-orcid":false,"given":"\u0130brahim Halil","family":"K\u0131l\u0131\u00e7","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science and Letters, Gaziantep University, Gaziantep 27310, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11786361231200836","DOI":"10.1177\/11786361231200836","article-title":"Candida auris: An Emerging Multidrug-Resistant Fungal Pathogen in the United States and the Urgent Call for Action","volume":"16","author":"Kumar","year":"2023","journal-title":"Microbiol. 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Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/15\/7\/692\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T07:28:00Z","timestamp":1784186880000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/15\/7\/692"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,16]]},"references-count":28,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["antibiotics15070692"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics15070692","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,16]]}}}