{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T04:47:15Z","timestamp":1785646035327,"version":"3.56.0"},"reference-count":60,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018712","name":"\u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy","doi-asserted-by":"crossref","award":["32153\/8"],"award-info":[{"award-number":["32153\/8"]}],"id":[{"id":"10.13039\/501100018712","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32153\/8"],"award-info":[{"award-number":["32153\/8"]}],"id":[{"id":"https:\/\/ror.org\/051h0cw83","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Melittis melissophyllum L. (bastard balm) is a perennial herb belonging to the Lamiaceae family. This study investigated the phytochemical composition, antioxidant properties, antimicrobial activity, and cytotoxic effects of a 50% (v\/v) ethanol\u2013water extract of M. melissophyllum. Methods: The phytochemical profiles were characterized by spectrophotometric analysis, while individual polyphenolic compounds were identified and quantified using LC\u2013MS. Antioxidant activity was assessed using the DPPH radical scavenging and FRAP assays. The cytotoxic effects were evaluated in normal human foreskin fibroblasts (BJ) and two human cancer cell lines, HCT-116 (colorectal carcinoma) and OVCAR-3 (ovarian adenocarcinoma). The antimicrobial efficacy of M. melissophyllum extracts was assessed against a panel of Gram-positive and Gram-negative bacterial strains, as well as a representative yeast species. Results: The results revealed a high content of polyphenolic compounds in the tested extract. Cytotoxicity assays demonstrated good tolerability toward normal cells while indicating inhibitory effects on tumor-derived cell lines. In addition, the extract exhibited limited but measurable strain-dependent antimicrobial activity. Conclusions: Collectively, these findings suggest that M. melissophyllum represents a promising source of bioactive compounds with potential medicinal applications.<\/jats:p>","DOI":"10.3390\/antibiotics15080742","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:20:18Z","timestamp":1785486018000},"page":"742","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Chemical Profiling and Biological Assessment of Melittis melissophyllum L. (Lamiaceae): Antioxidant, Antimicrobial and Cytotoxic Activities"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8695-6123","authenticated-orcid":false,"given":"Daniela","family":"Benedec","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4098-6962","authenticated-orcid":false,"given":"Irina","family":"Ielciu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7919-7381","authenticated-orcid":false,"given":"Emoke","family":"Pall","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400001 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6309-9181","authenticated-orcid":false,"given":"Adriana Dalila","family":"Criste","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400001 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9009-8413","authenticated-orcid":false,"given":"Adriana Cristina","family":"Urcan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400001 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7660-9993","authenticated-orcid":false,"given":"Ilioara","family":"Oniga","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timea Henrietta","family":"Dezs\u0151 (Bab)","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4370-4668","authenticated-orcid":false,"given":"Ramona Flavia","family":"Burtescu","sequence":"additional","affiliation":[{"name":"PlantExtrakt Ltd., 407059 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5407-8001","authenticated-orcid":false,"given":"Daniela","family":"Hanganu","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"ref_1","first-page":"99","article-title":"Forests Vegetation, Forest Habitats of Community Interest in North-West Romania, Included in the Project \u201cPriority Habitats of Forest Steppe and Hilly Piedmonts\u201d, Potential Threats and Monitoring of Conservation Status (II)","volume":"23","author":"Burescu","year":"2019","journal-title":"Ann. 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