{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:25:54Z","timestamp":1787199954681,"version":"build-2736575974"},"reference-count":59,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanghai Municipal Science and Technology Major Project"},{"name":"National Key Research and Development Program of China","award":["2025YFA0923200"],"award-info":[{"award-number":["2025YFA0923200"]}]},{"name":"Shanghai Sci-Tech Inno Center for Infection & Immunity","award":["SSIII-2024A02"],"award-info":[{"award-number":["SSIII-2024A02"]}]},{"name":"Shanghai Sci-Tech Inno Center for Infection & Immunity","award":["SSIII-2024A01"],"award-info":[{"award-number":["SSIII-2024A01"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81903529"],"award-info":[{"award-number":["81903529"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["21977029"],"award-info":[{"award-number":["21977029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["81903529"],"award-info":[{"award-number":["81903529"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["21977029"],"award-info":[{"award-number":["21977029"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"crossref","award":["JKF01251815"],"award-info":[{"award-number":["JKF01251815"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100013314","name":"111 Project","doi-asserted-by":"crossref","award":["B18022"],"award-info":[{"award-number":["B18022"]}],"id":[{"id":"10.13039\/501100013314","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/01h531d29","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: Endophytic fungi are important sources of structurally diverse antimicrobial natural products, but their potential to yield antitubercular scaffolds remains insufficiently explored. This study aimed to uncover new decalin polyketides from the endophytic fungus, Trichoderma longicollum 17007, and to evaluate their biological activity. Methods: Feature-based molecular networking (FBMN) analysis of the ethyl acetate extract from rice-based cultures was performed using trichoharzin as a seed compound to guide targeted isolation. Structures were established by HRMS, 1D and 2D NMR spectroscopy, NOESY analysis, and quantum chemical ECD calculations. Cytotoxicity and activity against the avirulent Mycobacterium tuberculosis H37Ra strain were evaluated, and genome mining was performed to identify a candidate biosynthetic gene cluster. Results: Six new decalin polyketides, tandyukisins K\u2013P (1\u20136), and two known analogues, trichoharzin (7) and tandyukisin J (8), were isolated. Compounds 1\u20136 are C1-O-acetylated analogues bearing a 3-methylpentenedioate-type acyl side chain. Comparative 13C NMR analysis revealed empirical features useful for assigning side-chain attachment site, double-bond position and geometry. Tandyukisins K (1) and P (6) showed moderate activity against the avirulent M. tuberculosis H37Ra strain, with a MIC value of 12.5 \u03bcg\/mL. Conclusions: These findings expand the structural diversity of decalin polyketides and provide the first evidence of antitubercular activity by this compound family.<\/jats:p>","DOI":"10.3390\/antibiotics15080789","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T15:48:36Z","timestamp":1786722516000},"page":"789","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tandyukisins K\u2013P, New Decalin Polyketides from Trichoderma longicollum 17007 with Antitubercular Activity Against Mycobacterium tuberculosis H37Ra"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9350-3074","authenticated-orcid":false,"given":"Junjie","family":"Yu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangqian","family":"Ning","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchang","family":"Di","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China"},{"name":"Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbo","family":"Feng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongxuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menghan","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sicheng","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenglin","family":"Jiang","sequence":"additional","affiliation":[{"name":"The Key Lab for Research & Development of Actinomycete Resources, Yunnan Institute of Microbiology, Yunnan University, Chenggong District, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1847-252X","authenticated-orcid":false,"given":"Yi","family":"Jiang","sequence":"additional","affiliation":[{"name":"The Key Lab for Research & Development of Actinomycete Resources, Yunnan Institute of Microbiology, Yunnan University, Chenggong District, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Hsiang","sequence":"additional","affiliation":[{"name":"School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiazhen","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China"},{"name":"Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China"},{"name":"Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixin","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueting","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4999-3598","authenticated-orcid":false,"given":"Xuelian","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China"},{"name":"Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6628-8874","authenticated-orcid":false,"given":"Guoliang","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2026, July 29). Tuberculosis. WHO Fact Sheet. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/tuberculosis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Jakubczyk, D., and Dussart, F. (2020). Selected fungal natural products with antimicrobial properties. Molecules, 25.","DOI":"10.3390\/molecules25040911"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1111\/j.1469-8137.2009.02773.x","article-title":"Fungal endophytes: Diversity and functional roles","volume":"182","author":"Rodriguez","year":"2009","journal-title":"New Phytol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2955","DOI":"10.1007\/s00253-015-6487-3","article-title":"Plant-endophyte symbiosis, an ecological perspective","volume":"99","author":"Wani","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1021\/np058128n","article-title":"Natural products from plant-associated microorganisms: Distribution, structural diversity, bioactivity, and implications of their occurrence","volume":"69","author":"Gunatilaka","year":"2006","journal-title":"J. Nat. Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"107462","DOI":"10.1016\/j.biotechadv.2019.107462","article-title":"A critical review on exploiting the pharmaceutical potential of plant endophytic fungi","volume":"39","author":"Gupta","year":"2020","journal-title":"Biotechnol. Adv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/nrmicro797","article-title":"Trichoderma species--opportunistic, avirulent plant symbionts","volume":"2","author":"Harman","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"11502","DOI":"10.1021\/acs.jafc.3c03240","article-title":"Assessing the biosynthetic inventory of the biocontrol fungus Trichoderma afroharzianum T22","volume":"71","author":"Han","year":"2023","journal-title":"J. Agric. Food. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1039\/D5NP00017C","article-title":"Advances in the discovery and study of Trichoderma natural products for biological control applications","volume":"42","author":"Jin","year":"2025","journal-title":"Nat. Prod. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Lai, C., Chen, J., Liu, J., Tian, D., Lan, D., Liu, T., Wu, B., Bi, H., and Tang, J. (2022). New polyketides from a hydrothermal vent sediment fungus Trichoderma sp. JWM29-10-1 and their antimicrobial effects. Mar. Drugs, 20.","DOI":"10.3390\/md20110720"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Yin, Y., Tan, Q., Wu, J., Chen, T., Yang, W., She, Z., and Wang, B. (2023). The polyketides with antimicrobial activities from a mangrove endophytic fungus Trichoderma lentiforme ML-P8-2. Mar. Drugs, 21.","DOI":"10.3390\/md21110566"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1099\/mic.0.053629-0","article-title":"Secondary metabolism in Trichoderma--a genomic perspective","volume":"158","author":"Mukherjee","year":"2012","journal-title":"Microbiology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"153","DOI":"10.33585\/cmy.75204","article-title":"Trichoderma longicollum (Hypocreales), a new species from Taiwan","volume":"75","author":"Wei","year":"2023","journal-title":"Czech Mycol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1007\/s10600-025-04840-x","article-title":"Metabolites from the soil-derived fungus Trichoderma longicollum BCRC 19F0006","volume":"61","author":"Cheng","year":"2025","journal-title":"Chem. Nat. Compd."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"W46","DOI":"10.1093\/nar\/gkad344","article-title":"antiSMASH 7.0: New and improved predictions for detection, regulation, chemical structures and visualisation","volume":"51","author":"Blin","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.cell.2014.06.034","article-title":"Insights into secondary metabolism from a global analysis of prokaryotic biosynthetic gene clusters","volume":"158","author":"Cimermancic","year":"2014","journal-title":"Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1016\/j.fgb.2010.06.003","article-title":"SMURF: Genomic mapping of fungal secondary metabolite clusters","volume":"47","author":"Khaldi","year":"2010","journal-title":"Fungal Genet. Biol."},{"key":"ref_18","first-page":"9645","article-title":"Genomes to natural products PRediction informatics for secondary metabolomes (PRISM)","volume":"43","author":"Skinnider","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/nbt.3597","article-title":"Sharing and community curation of mass spectrometry data with global natural products social molecular networking","volume":"34","author":"Wang","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/s41587-019-0375-9","article-title":"Mass spectrometry searches using MASST","volume":"38","author":"Wang","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Atencio, L.A., Boya, P.C., Martin, H.C., Mej\u00eda, L.C., Dorrestein, P.C., and Guti\u00e9rrez, M. (2020). Genome mining, microbial interactions, and molecular networking reveals new dibromoalterochromides from strains of Pseudoalteromonas of Coiba National Park-Panama. Mar. Drugs, 18.","DOI":"10.3390\/md18090456"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/s41592-020-0933-6","article-title":"Feature-based molecular networking in the GNPS analysis environment","volume":"17","author":"Nothias","year":"2020","journal-title":"Nat. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3832","DOI":"10.1038\/s41467-021-23953-9","article-title":"Ion identity molecular networking for mass spectrometry-based metabolomics in the GNPS environment","volume":"12","author":"Schmid","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"19609","DOI":"10.1002\/anie.202103878","article-title":"Discovery of neuritogenic securinega alkaloids from Flueggea suffruticosa by a building blocks-based molecular network strategy","volume":"60","author":"He","year":"2021","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1021\/acs.jnatprod.7b00737","article-title":"Bioactivity-based molecular networking for the discovery of drug leads in natural product bioassay-guided fractionation","volume":"81","author":"Nothias","year":"2018","journal-title":"J. Nat. Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7925","DOI":"10.1016\/S0040-4039(00)61513-7","article-title":"Trichoharzin, a new polyketide produced by the imperfect fungus Trichoderma harzianum separated from the marine sponge Mycale cecilia","volume":"34","author":"Kobayashi","year":"1993","journal-title":"Tetrahedron Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.tetlet.2013.11.107","article-title":"Tandyukisin, a novel ketoaldehyde decalin derivative, produced by a marine sponge-derived Trichoderma harzianum","volume":"55","author":"Yamada","year":"2014","journal-title":"Tetrahedron Lett."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3231","DOI":"10.3390\/md13053231","article-title":"Determination of the chemical structures of tandyukisins B\u2013D, isolated from a marine sponge-derived fungus","volume":"13","author":"Yamada","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5070","DOI":"10.1016\/j.tetlet.2016.10.004","article-title":"Tandyukisins E and F, novel cytotoxic decalin derivatives isolated from a marine sponge-derived fungus","volume":"57","author":"Suzue","year":"2016","journal-title":"Tetrahedron Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4256","DOI":"10.1080\/14786419.2025.2509885","article-title":"Decalin derivatives from an endophytic fungus Trichoderma harzianum GLQ03 and their anti-inflammatory activities","volume":"40","author":"Li","year":"2026","journal-title":"Nat. Prod. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s40694-018-0063-4","article-title":"Identification of the decumbenone biosynthetic gene cluster in Penicillium decumbens and the importance for production of calbistrin","volume":"5","author":"Grijseels","year":"2018","journal-title":"Fungal Biol. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8851","DOI":"10.1002\/anie.202016525","article-title":"One polyketide synthase, two distinct products: Trans-acting enzyme-controlled product divergence in calbistrin biosynthesis","volume":"60","author":"Tao","year":"2021","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"15837","DOI":"10.1021\/jacs.6b10452","article-title":"Biochemical characterization of a eukaryotic decalin-forming Diels-Alderase","volume":"138","author":"Li","year":"2016","journal-title":"J. Am. Chem. Soc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1021\/cb400541z","article-title":"Complexity generation in fungal polyketide biosynthesis: A spirocycle-forming P450 in the concise pathway to the antifungal drug griseofulvin","volume":"8","author":"Cacho","year":"2013","journal-title":"ACS Chem. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1039\/C5SC04027B","article-title":"The molecular steps of citrinin biosynthesis in fungi","volume":"7","author":"He","year":"2016","journal-title":"Chem. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.1111\/j.1742-4658.2006.05218.x","article-title":"Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism","volume":"273","author":"Mack","year":"2006","journal-title":"FEBS J."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Jennings, E.A., Cao, E., Romenskaia, I., and Ryan, R.O. (2023). Characterization of trans-3-methylglutaconyl CoA-dependent protein acylation. Metabolites, 13.","DOI":"10.3390\/metabo13070862"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Jones, D.E., Jennings, E.A., and Ryan, R.O. (2022). Diversion of acetyl CoA to 3-methylglutaconic acid caused by discrete inborn errors of metabolism. Metabolites, 12.","DOI":"10.3390\/metabo12050377"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Hamid, A., Ali, G., Shikari, A., Saleem, S., Wani, S.H., and Wani, S. (2025). Extraction of high-quality genomic DNA from plants using modified CTAB-based method. Detection of Plant Viruses: Advanced Techniques, Springer.","DOI":"10.1007\/978-1-0716-4390-7"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6115","DOI":"10.1093\/nar\/20.22.6115","article-title":"A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues","volume":"20","author":"Bahnweg","year":"1992","journal-title":"Nucleic Acids Res."},{"key":"ref_41","first-page":"315","article-title":"Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics","volume":"Volume 31","author":"White","year":"1990","journal-title":"PCR Protocols"},{"key":"ref_42","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5167","DOI":"10.1007\/s00253-019-09821-z","article-title":"Genome- and MS-based mining of antibacterial chlorinated chromones and xanthones from the phytopathogenic fungus Bipolaris sorokiniana strain 11134","volume":"103","author":"Han","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_45","first-page":"684\u2212693","article-title":"New N-acylated aminoalkanoic acids from tea roots derived biocontrol agent Clonostachys rosea 15020","volume":"9","author":"Yu","year":"2024","journal-title":"Synth. Syst. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"W32","DOI":"10.1093\/nar\/gkaf334","article-title":"antiSMASH 8.0: Extended gene cluster detection capabilities and analyses of chemistry, enzymology, and regulation","volume":"53","author":"Blin","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and applications. BMC Bioinf., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2534","DOI":"10.1093\/bioinformatics\/btn323","article-title":"ProteoWizard: Open source software for rapid proteomics tools development","volume":"24","author":"Kessner","year":"2008","journal-title":"Bioinformatics"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Pluskal, T., Castillo, S., Villar-Briones, A., and Ore\u0161i\u010d, M. (2010). MZmine 2: Modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data. BMC Bioinf., 11.","DOI":"10.1186\/1471-2105-11-395"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"ref_51","unstructured":"Frisch, M.J., Trucks, G.W., Schlegel, H.B., Scuseria, G.E., Robb, M.A., Cheeseman, J.R., Scalmani, G., Barone, V., Mennucci, B., and Petersson, G.A. (2010). Gaussian 09, revision D.01, Gaussian, Inc."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5648","DOI":"10.1063\/1.464913","article-title":"Density-functional thermochemistry. III. The role of exact exchange","volume":"98","author":"Becke","year":"1993","journal-title":"J. Chem. Phys."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1103\/PhysRevB.37.785","article-title":"Development of the Colle-Salvetti correlation-energy formula into a functional of the electron density","volume":"37","author":"Lee","year":"1988","journal-title":"Phys. Rev. B"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2999","DOI":"10.1021\/cr9904009","article-title":"Quantum mechanical continuum solvation models","volume":"105","author":"Tomasi","year":"2005","journal-title":"Chem. Rev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1103\/PhysRevLett.52.997","article-title":"Density-functional theory for time-dependent systems","volume":"52","author":"Runge","year":"1984","journal-title":"Phys. Rev. Lett."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1002\/chir.22600","article-title":"Good computational practice in the assignment of absolute configurations by TDDFT calculations of ECD spectra","volume":"28","author":"Pescitelli","year":"2016","journal-title":"Chirality"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.synbio.2019.09.001","article-title":"Brocaeloid D, a novel compound isolated from a wheat pathogenic fungus, Microdochium majus 99049","volume":"4","author":"Zhang","year":"2019","journal-title":"Synth. Syst. Biotechnol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1039\/a809656b","article-title":"A water-soluble tetrazolium salt useful for colorimetric cell viability assay","volume":"36","author":"Tominaga","year":"1999","journal-title":"Anal. Commun."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"7105","DOI":"10.1039\/c1jm10575b","article-title":"Apoferritin-camouflaged Pt nanoparticles: Surface effects on cellular uptake and cytotoxicity","volume":"21","author":"Liu","year":"2011","journal-title":"J. Mater. Chem."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/15\/8\/789\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:13:52Z","timestamp":1787199232000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/15\/8\/789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,14]]},"references-count":59,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["antibiotics15080789"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics15080789","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,14]]}}}