{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:09:20Z","timestamp":1788930560871,"version":"build-2803163510"},"reference-count":204,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["RS-2021-NR060118"],"award-info":[{"award-number":["RS-2021-NR060118"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"award":["RS-2021-NR060118"],"award-info":[{"award-number":["RS-2021-NR060118"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Korea government","award":["RS-2026-25587593"],"award-info":[{"award-number":["RS-2026-25587593"]}]},{"award":["RS-2026-25587593"],"award-info":[{"award-number":["RS-2026-25587593"]}],"id":[{"id":"https:\/\/ror.org\/01wpjm123","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"crossref","award":["RS-2025-02373103"],"award-info":[{"award-number":["RS-2025-02373103"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"crossref"}]},{"award":["RS-2025-02373103"],"award-info":[{"award-number":["RS-2025-02373103"]}],"id":[{"id":"https:\/\/ror.org\/00c5s4a53","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Vibriosis causes substantial losses in marine and brackish-water aquaculture, while increasing antimicrobial resistance limits the effectiveness of conventional antibiotic treatment. Biofilm formation and quorum-sensing-controlled virulence further contribute to the persistence of pathogenic Vibrio species. Prophages integrated into Vibrio genomes encode proteins and regulatory elements that may provide alternative approaches for controlling these pathogens. This review evaluates endolysins, polysaccharide depolymerases, nucleases, holins, spanins, tailocins, regulatory proteins, and small RNAs associated with prophages and related phages. The available evidence was classified according to molecular origin to distinguish validated prophage-derived molecules from those obtained from temperate or lytic phages and from molecules characterized in non-Vibrio bacteria. Endolysins and depolymerases can disrupt bacterial cells and biofilm matrices, whereas prophage regulatory elements may influence quorum sensing, adhesion, motility, toxin production, and secretion systems. However, the evidence directly supporting prophage-derived anti-Vibrio agents remains limited. Most experimentally demonstrated activity has been reported for endolysins and one validated depolymerase, and many of these molecules originated from lytic rather than temperate phages. No direct Vibrio-specific evidence is currently available for phage-derived nucleases, holins, spanins, or tailocins as isolated control agents. Prophage genomes nevertheless provide an extensive source of regulatory and antimicrobial candidates for further investigation. Progress toward aquaculture application will require experimental validation, effective delivery methods, safety assessment, scalable production, and clear regulatory standards.<\/jats:p>","DOI":"10.3390\/antibiotics15090874","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T13:53:28Z","timestamp":1788789208000},"page":"874","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prophage-Derived Molecules as Anti-Vibrio Agents in Aquaculture: Mechanisms of Biofilm Control and Virulence Suppression"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9314-9124","authenticated-orcid":false,"given":"Nazia","family":"Tabassum","sequence":"first","affiliation":[{"name":"Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aqib","family":"Javaid","sequence":"additional","affiliation":[{"name":"Interdisciplinary Program of Marine and Fisheries Sciences and Convergent Technology, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abirami","family":"Karthikeyan","sequence":"additional","affiliation":[{"name":"Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8926-9449","authenticated-orcid":false,"given":"Minji","family":"Kim","sequence":"additional","affiliation":[{"name":"Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2465-8013","authenticated-orcid":false,"given":"Young-Mog","family":"Kim","sequence":"additional","affiliation":[{"name":"Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4902-3188","authenticated-orcid":false,"given":"Fazlurrahman","family":"Khan","sequence":"additional","affiliation":[{"name":"Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Interdisciplinary Program of Marine and Fisheries Sciences and Convergent Technology, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Ocean and Fisheries Development International Cooperation Institute, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"International Graduate Program of Fisheries Science, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e70039","DOI":"10.1111\/raq.70039","article-title":"The Current State of Knowledge of the Economic Impact of Diseases in Global Aquaculture","volume":"17","author":"Maezono","year":"2025","journal-title":"Rev. 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