{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T10:06:16Z","timestamp":1789553176730,"version":"build-2803163510"},"reference-count":24,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: Orthopaedic infections require accurate microbiological diagnosis for effective treatment. This study aimed to evaluate the impact of an accelerated protocol for culture harvesting and incubation on culture positivity rates in orthopaedic surgeries, comparing it to the traditional protocol. Methods: A single-center, 1:1 matched case\u2013control study was conducted at the Rubin Institute for Advanced Orthopaedics. Patients undergoing orthopaedic surgery with suspected infections were divided into two groups (108 patients): accelerated protocol (June\u2013November 2024) and traditional protocol (January\u2013June 2024). The time from operating room (OR) to incubation was significantly shorter in the accelerated group (89 min vs. 245 min, p &lt; 0.001). Culture positivity rates and microorganism profiles were compared. The temporal dynamics of culture positivity were evaluated using Kaplan\u2013Meier analysis across the entire cohort. A logistic regression analysis was carried out between the time from OR to incubation and culture positivity. Results: The accelerated protocol group had significantly higher culture positivity rates (70.4%) compared to the traditional group (48.1%) (p = 0.027). Microbial profiles were similar between groups. The probability of culture positivity across the entire cohort decreased by 6% for every 10-min delay after 100 min from OR. Regression analysis showed a significant inverse association between time and culture positivity (\u03b2 = \u22120.005, p = 0.016). Conclusions: The accelerated protocol significantly improved culture positivity rates without compromising diagnostic performance. Reducing the time from sample collection to incubation enhances pathogen detection across all pathogen types, potentially improving outcomes in orthopaedic infection management.<\/jats:p>","DOI":"10.3390\/antibiotics15090913","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:06:37Z","timestamp":1789549597000},"page":"913","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["How Does the Time from Intraoperative Culture Harvest to Incubation Impact Culture Positivity in Orthopaedic Surgeries? A Case\u2013Control Study"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4607-354X","authenticated-orcid":false,"given":"Amir Human","family":"Hoveidaei","sequence":"first","affiliation":[{"name":"International Center for Limb Lengthening, Rubin Institute for Advanced Orthopedics, Sinai Hospital of Baltimore, Schoeneman Building, 2nd Floor, 2401 West Belvedere Avenue, Baltimore, MD 21215, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"James K.","family":"Conway","sequence":"additional","affiliation":[{"name":"International Center for Limb Lengthening, Rubin Institute for Advanced Orthopedics, Sinai Hospital of Baltimore, Schoeneman Building, 2nd Floor, 2401 West Belvedere Avenue, Baltimore, MD 21215, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3665-9328","authenticated-orcid":false,"given":"Kyree","family":"Day","sequence":"additional","affiliation":[{"name":"International Center for Limb Lengthening, Rubin Institute for Advanced Orthopedics, Sinai Hospital of Baltimore, Schoeneman Building, 2nd Floor, 2401 West Belvedere Avenue, Baltimore, MD 21215, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3554-6209","authenticated-orcid":false,"given":"Elizabeth K.","family":"Tissingh","sequence":"additional","affiliation":[{"name":"Limb Reconstruction and Bone Infection Service, The Royal Ntaional Orthopaedic Hospital, Brockley Hill, London HA7 4LP, UK"},{"name":"King\u2019s Global Health Partnerships, School of Life Course and Population Sciences, King\u2019s College London, London SE5 9RJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet D.","family":"Conway","sequence":"additional","affiliation":[{"name":"International Center for Limb Lengthening, Rubin Institute for Advanced Orthopedics, Sinai Hospital of Baltimore, Schoeneman Building, 2nd Floor, 2401 West Belvedere Avenue, Baltimore, MD 21215, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1016\/j.jseint.2024.06.018","article-title":"Does preoperative diagnosis impact patient outcomes following reverse total shoulder arthroplasty?","volume":"9","author":"Michaelson","year":"2024","journal-title":"JSES Int."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1308\/rcsann.2015.0050","article-title":"The burden of bone, native joint and soft tissue infections on orthopaedic emergency referrals in a city hospital","volume":"98","author":"Howell","year":"2016","journal-title":"Ann. R. Coll. Surg. Engl."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1038\/s41579-021-00578-9","article-title":"Optimizing antimicrobial use: Challenges, advances and opportunities","volume":"19","author":"Rawson","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Miller, J.M., Binnicker, M.J., Campbell, S., Carroll, K.C., Chapin, K.C., Gonzalez, M.D., Harrington, A., Jerris, R.C., Kehl, S.C., and Leal, S.M. (Clin. Infect. Dis., 2024). Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2024 Update by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM), Clin. Infect. Dis., Online ahead of print.","DOI":"10.1093\/cid\/ciae104"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1056\/NEJMra2203477","article-title":"Periprosthetic Joint Infection","volume":"388","author":"Patel","year":"2023","journal-title":"N. Engl. J. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.injury.2017.08.040","article-title":"Fracture-related infection: A consensus on definition from an international expert group","volume":"49","author":"Metsemakers","year":"2018","journal-title":"Injury"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Van Cauter, M., Cornu, O., Yombi, J.C., Rodriguez-Villalobos, H., and Kaminski, L. (2018). The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0192048"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1007\/s10096-025-05136-2","article-title":"Enhancing diagnostic accuracy for periprosthetic joint infections with an alternative homogenization approach","volume":"44","author":"Goes","year":"2025","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e0114324","DOI":"10.1128\/jcm.01143-24","article-title":"Prolonged incubation time unwarranted for acute periprosthetic joint infections","volume":"63","author":"Morreel","year":"2025","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"(2011). Workgroup Convened by the Musculoskeletal Infection Society. New definition for periprosthetic joint infection. J. Arthroplast., 26, 1136\u20131138.","DOI":"10.1016\/j.arth.2011.09.026"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1302\/0301-620X.103B1.BJJ-2020-1381.R1","article-title":"The EBJIS definition of periprosthetic joint infection","volume":"103-B","author":"McNally","year":"2021","journal-title":"Bone Jt. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"977","DOI":"10.2106\/JBJS.20.00409","article-title":"Providing an Evidence Base for Tissue Sampling and Culture Interpretation in Suspected Fracture-Related Infection","volume":"103","author":"Dudareva","year":"2021","journal-title":"J. Bone Jt. Surg. Am."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1302\/2046-3758.119.BJR-2022-0078.R1","article-title":"Diagnosing periprosthetic joint infections: A comparison of infection definitions: EBJIS 2021, ICM 2018, and IDSA 2013","volume":"11","author":"Sigmund","year":"2022","journal-title":"Bone Jt. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"106520","DOI":"10.1016\/j.jinf.2025.106520","article-title":"Less haste, more speed: Does delayed blood culture transport time lead to adverse incubation times or yield?","volume":"91","author":"Deas","year":"2025","journal-title":"J. Infect."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ling, C.L., Roberts, T., Soeng, S., Cusack, T.P., Dance, D.A.B., Lee, S.J., Reed, T.A.N., Hinfonthong, P., Sihalath, S., and Sengduangphachanh, A. (2021). Impact of delays to incubation and storage temperature on blood culture results: A multi-centre study. BMC Infect. Dis., 21.","DOI":"10.1186\/s12879-021-05872-8"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"104193","DOI":"10.1016\/j.otsr.2025.104193","article-title":"A nine-year retrospective study of bacterial epidemiology and culture optimization in four orthopaedic surgery departments","volume":"111","author":"Robinet","year":"2025","journal-title":"Orthop. Traumatol. Surg. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.arth.2013.03.037","article-title":"Multiple cultures and extended incubation for hip and knee arthroplasty revision: Impact on clinical care","volume":"28","author":"DeHaan","year":"2013","journal-title":"J. Arthroplast."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"107","DOI":"10.2106\/JBJS.22.00766","article-title":"Time to Positivity of Cultures Obtained for Periprosthetic Joint Infection","volume":"105","author":"Tarabichi","year":"2023","journal-title":"J. Bone Jt. Surg. Am."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1128\/JCM.01766-13","article-title":"Optimal culture incubation time in orthopedic device-associated infections: A retrospective analysis of prolonged 14-day incubation","volume":"52","author":"Schwotzer","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Baez, C., MacDonell, R., Tishad, A., Prieto, H.A., Miley, E.N., Deen, J.T., Gray, C.F., Parvataneni, H.K., and Pulido, L. (2024). Comparison of Five-Day vs. Fourteen-Day Incubation of Cultures for Diagnosis of Periprosthetic Joint Infection in Hip Arthroplasty. J. Clin. Med., 13.","DOI":"10.3390\/jcm13154467"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3531","DOI":"10.1016\/j.arth.2018.06.018","article-title":"Culturing Periprosthetic Joint Infection: Number of Samples, Growth Duration, and Organisms","volume":"33","author":"Kheir","year":"2018","journal-title":"J. Arthroplast."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Venturelli, C., Righi, E., Borsari, L., Aggazzotti, G., Busani, S., Mussini, C., Rumpianesi, F., Rossolini, G.M., and Girardis, M. (2017). Impact of Pre-Analytical Time on the Recovery of Pathogens from Blood Cultures: Results from a Large Retrospective Survey. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0169466"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e0143022","DOI":"10.1128\/spectrum.01430-22","article-title":"Antibiotic Stewardship in Treatment of Osteoarticular Infections Based on Local Epidemiology and Bacterial Growth Times","volume":"10","author":"Vidal","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.ijid.2017.04.020","article-title":"Opportunities for system level improvement in antibiotic use across the surgical pathway","volume":"60","author":"Charani","year":"2017","journal-title":"Int. J. 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