{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:16:49Z","timestamp":1787505409916,"version":"build-2736575974"},"reference-count":24,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005278","name":"Universidad de Antioquia","doi-asserted-by":"publisher","award":["SUSTAINABILITY PROGRAM"],"award-info":[{"award-number":["SUSTAINABILITY PROGRAM"]}],"id":[{"id":"10.13039\/501100005278","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","award":["Doctoral Formation Program (567-2012)"],"award-info":[{"award-number":["Doctoral Formation Program (567-2012)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Bacterial resistance is caused by several biochemical factors, the formation of biofilm being one of the main causes. This process is triggered by Quorum Sensing (QS), through the production of endogenous molecules, although other substances such as natural products can also do this. In this work, we aimed to determine whether some drugs are involved in the induction of biofilm formation in Klebsiella pneumoniae ATCC 13884, and thus, increase bacterial resistance. For this, the effect of 22 drugs on K. pneumoniae ATCC 13884 growth was determined at sub-plasmatic concentrations; the production of autoinducer lactones was established by HPLC and with a biosensor. The induction of biofilm formation was determined through crystal violet assay at 585 nm in a microplate reader and using urethral catheters. According to the in vitro assays, some drugs were found to induce biofilm formation in K. pneumoniae ATCC 13884. The effect of acetaminophen, hydrochlorothiazide, and progesterone stood out. The first drug caused several changes in the biochemistry of K. pneumoniae ATCC 13884 related to QS: high synthesis of N-hexanoyl-homoserine lactone, increasing bacterial populations by 27% and biofilm formation by 49%, and a more gentamicin resistant biofilm. Furthermore, it increased the colonization area of urethral catheters. Hydrochlorothiazide showed the biggest increase in the induction of biofilm formation of 51%, and progesterone displayed the greatest ability to provoke bacterial mass adherence but had no effects on K. pneumoniae ATCC 13884 bacterial population growth.<\/jats:p>","DOI":"10.3390\/antibiotics7040103","type":"journal-article","created":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T11:43:44Z","timestamp":1543405424000},"page":"103","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Induction of Biofilm Formation in Klebsiella pneumoniae ATCC 13884 by Several Drugs: The Possible Role of Quorum Sensing Modulation"],"prefix":"10.3390","volume":"7","author":[{"given":"Elizabeth","family":"Cadavid","sequence":"first","affiliation":[{"name":"Grupo de Qu\u00edmica Org\u00e1nica de Productos Naturales, Instituto de Qu\u00edmica, Universidad de Antioquia, Calle 67 No. 53-10, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2752-4931","authenticated-orcid":false,"given":"Sara M.","family":"Robledo","sequence":"additional","affiliation":[{"name":"PECET, Instituto de Investigaciones M\u00e9dicas, Facultad de Medicina, Universidad de Antioquia, Calle 62 No. 52-59, Lab 632, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiston","family":"Qui\u00f1ones","sequence":"additional","affiliation":[{"name":"Grupo de Qu\u00edmica Org\u00e1nica de Productos Naturales, Instituto de Qu\u00edmica, Universidad de Antioquia, Calle 67 No. 53-10, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9530-8395","authenticated-orcid":false,"given":"Fernando","family":"Echeverri","sequence":"additional","affiliation":[{"name":"Grupo de Qu\u00edmica Org\u00e1nica de Productos Naturales, Instituto de Qu\u00edmica, Universidad de Antioquia, Calle 67 No. 53-10, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"McConville, T.H., Sullivan, S.B., Gomez-Simmonds, A., Whittier, S., and Uhlemann, A.C. 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