{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:31:40Z","timestamp":1787027500793,"version":"build-2736575974"},"reference-count":66,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T00:00:00Z","timestamp":1559088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Persistent methicillin-resistant Staphylococcus aureus (MRSA) bacteremia (PB) represents an important subset of S. aureus infection and correlates with poor clinical outcomes. MRSA isolates from patients with PB differ significantly from those of resolving bacteremia (RB) with regard to several in vitro phenotypic and genotypic profiles. For instance, PB strains exhibit less susceptibility to cationic host defense peptides and vancomycin (VAN) killing under in vivo-like conditions, greater damage to endothelial cells, thicker biofilm formation, altered growth rates, early activation of many global virulence regulons (e.g., sigB, sarA, sae and agr) and higher expression of purine biosynthesis genes (e.g., purF) than RB strains. Importantly, PB strains are significantly more resistant to VAN treatment in experimental infective endocarditis as compared to RB strains, despite similar VAN minimum inhibitory concentrations (MICs) in vitro. Here, we review relevant phenotypic and genotypic characteristics related to the PB outcome. These and future insights may improve our understanding of the specific mechanism(s) contributing to the PB outcome, and aid in the development of novel therapeutic and preventative measures against this life-threatening infection.<\/jats:p>","DOI":"10.3390\/antibiotics8020071","type":"journal-article","created":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T11:31:28Z","timestamp":1559129488000},"page":"71","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Phenotypic and Genotypic Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Related to Persistent Endovascular Infection"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3571-8058","authenticated-orcid":false,"given":"Liang","family":"Li","sequence":"first","affiliation":[{"name":"Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Yeaman","sequence":"additional","affiliation":[{"name":"Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA"},{"name":"David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1968-0192","authenticated-orcid":false,"given":"Arnold S.","family":"Bayer","sequence":"additional","affiliation":[{"name":"Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA"},{"name":"Division of Molecular Medicine, Harbor-University of California, Los Angeles (UCLA) Medical Center, Torrance, CA 90502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan Q.","family":"Xiong","sequence":"additional","affiliation":[{"name":"Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA"},{"name":"Division of Molecular Medicine, Harbor-University of California, Los Angeles (UCLA) Medical Center, Torrance, CA 90502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1056\/NEJM199808203390806","article-title":"Staphylococcus aureus infections","volume":"339","author":"Lowy","year":"1998","journal-title":"N. Engl. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3012","DOI":"10.1001\/jama.293.24.3012","article-title":"Staphylococcus aureus endocarditis: A consequence of medical progress","volume":"293","author":"Fowler","year":"2005","journal-title":"JAMA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S18","DOI":"10.1016\/j.ijantimicag.2008.06.006","article-title":"Staphylococcus aureus bacteremia with known sources","volume":"32","author":"Rubinstein","year":"2008","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1097\/01.md.0000091184.93122.09","article-title":"Staphylococcus aureus bacteremia: Recurrence and the impact of antibiotic treatment in a prospective multicenter study","volume":"82","author":"Chang","year":"2003","journal-title":"Medicine"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1469-0691.2005.01138.x","article-title":"Current and emerging serious Gram-positive infections","volume":"11","author":"Menichetti","year":"2005","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1001\/jama.298.15.1763","article-title":"Invasive methicillin-resistant Staphylococcus aureus infections in the United States","volume":"298","author":"Klevens","year":"2007","journal-title":"JAMA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1086\/595738","article-title":"Phenotypic and genotypic characteristics of persistent methicillin-resistant Staphylococcus aureus bacteremia in vitro and in an experimental endocarditis model","volume":"199","author":"Xiong","year":"2009","journal-title":"J. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1001\/archinte.167.17.1861","article-title":"Persistent Staphylococcus aureus bacteremia: An analysis of risk factors and outcomes","volume":"167","author":"Hawkins","year":"2007","journal-title":"Arch. Intern. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1086\/423145","article-title":"Persistent bacteremia due to methicillin-resistant Staphylococcus aureus infection is associated with agr dysfunction and low-level in vitro resistance to thrombin-induced platelet microbicidal protein","volume":"190","author":"Fowler","year":"2004","journal-title":"J. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1080\/00365540500372846","article-title":"Persistence in Staphylococcus aureus bacteremia: incidence, characteristics of patients and outcome","volume":"38","author":"Khatib","year":"2006","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1128\/AAC.01028-10","article-title":"Combinatorial phenotypic signatures distinguish persistent from resolving methicillin-resistant Staphylococcus aureus bacteremia isolates","volume":"55","author":"Seidl","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5631","DOI":"10.1128\/AAC.05251-11","article-title":"Relationship of agr expression and function with virulence and vancomycin treatment outcomes in experimental endocarditis due to methicillin-resistant Staphylococcus aureus","volume":"55","author":"Seidl","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1086\/520088","article-title":"Potential associations between hematogenous complications and bacterial genotype in Staphylococcus aureus infection","volume":"196","author":"Fowler","year":"2007","journal-title":"J. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2890","DOI":"10.1128\/JCM.00905-08","article-title":"Associations between the genotypes of Staphylococcus aureus bloodstream isolates and clinical characteristics and outcomes of bacteremic patients","volume":"46","author":"Lalani","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1093\/jac\/dku547","article-title":"Early agr activation correlates with vancomycin treatment failure in multi-clonotype MRSA endovascular infections","volume":"70","author":"Abdelhady","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1128\/jcm.33.9.2233-2239.1995","article-title":"Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing","volume":"33","author":"Tenover","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1128\/JB.186.5.1518-1530.2004","article-title":"eBURST: Inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data","volume":"186","author":"Feil","year":"2004","journal-title":"J. Bacteriol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1093\/infdis\/jir389","article-title":"Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins","volume":"204","author":"Nienaber","year":"2011","journal-title":"J. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3556","DOI":"10.1128\/JCM.37.11.3556-3563.1999","article-title":"Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains","volume":"37","author":"Shopsin","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1128\/AAC.46.7.2155-2161.2002","article-title":"Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus","volume":"46","author":"Oliveira","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7341","DOI":"10.1128\/JB.183.24.7341-7353.2001","article-title":"Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and\/or sarA Loci","volume":"183","author":"Dunman","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1128\/JCM.44.5.1892-1895.2006","article-title":"Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay","volume":"44","author":"Francois","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.1086\/421092","article-title":"Accessory gene regulator group II polymorphism in methicillin-resistant Staphylococcus aureus is predictive of failure of vancomycin therapy","volume":"38","author":"Sakoulas","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Hernandez, D., Seidl, K., Corvaglia, A.R., Bayer, A.S., Xiong, Y.Q., and Francois, P. (2014). Genome sequences of sequence type 45 (ST45) persistent methicillin-resistant Staphylococcus aureus (MRSA) bacteremia strain 300-169 and ST45 resolving MRSA bacteremia strain 301-188. Genome Announc., 2.","DOI":"10.1128\/genomeA.00174-14"},{"key":"ref_25","unstructured":"Li, L., Abdelhady, W., Donegan, N.P., Seidl, K., Cheung, A., Zhou, Y.F., Yeaman, M.R., Bayer, A.S., and Xiong, Y.Q. (2018). Role of purine biosynthesis in persistent methicillin-resistant Staphylococcus aureus (MRSA) infection. J. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1086\/508210","article-title":"Regulation of Staphylococcus aureus alpha-toxin gene (hla) expression by agr, sarA, and sae in vitro and in experimental infective endocarditis","volume":"194","author":"Xiong","year":"2006","journal-title":"J. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1128\/AAC.02073-12","article-title":"Reduced vancomycin susceptibility in an in vitro catheter-related biofilm model correlates with poor therapeutic outcomes in experimental endocarditis due to methicillin-resistant Staphylococcus aureus","volume":"57","author":"Abdelhady","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3548","DOI":"10.1128\/IAI.68.6.3548-3553.2000","article-title":"In vitro resistance of Staphylococcus aureus to thrombin-induced platelet microbicidal protein is associated with alterations in cytoplasmic membrane fluidity","volume":"68","author":"Bayer","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4772","DOI":"10.1128\/IAI.01074-15","article-title":"Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB","volume":"83","author":"Xiong","year":"2015","journal-title":"Infect. Immun."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1111\/j.1462-5822.2011.01639.x","article-title":"In vitro endothelial cell damage is positively correlated with enhanced virulence and poor vancomycin responsiveness in experimental endocarditis due to methicillin-resistant Staphylococcus aureus","volume":"13","author":"Seidl","year":"2011","journal-title":"Cell Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1111\/j.1365-2958.2006.05441.x","article-title":"Prophages of Staphylococcus aureus Newman and their contribution to virulence","volume":"62","author":"Bae","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"12662","DOI":"10.1038\/s41598-018-31107-z","article-title":"Lysogenization of Staphylococcus aureus RN450 by phages varphi11 and varphi80alpha leads to the activation of the SigB regulon","volume":"8","author":"Fernandez","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Liu, Q., Yeo, W.S., and Bae, T. (2016). The SaeRS Two-component system of Staphylococcus aureus. Genes, 7.","DOI":"10.3390\/genes7100081"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Tuchscherr, L., Bischoff, M., Lattar, S.M., Noto Llana, M., Pfortner, H., Niemann, S., Geraci, J., Van de Vyver, H., Fraunholz, M.J., and Cheung, A.L. (2015). Sigma Factor SigB Is Crucial to Mediate Staphylococcus aureus Adaptation during Chronic Infections. PloS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004870"},{"key":"ref_35","unstructured":"Voet, D., Voet, J.G., and Pratt, C.W. (2008). Fundamentals of Biochemistry: Life at the Molecular Level, Wiley. [3rd ed.]."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"17719","DOI":"10.1074\/jbc.272.28.17719","article-title":"Amidophosphoribosyltransferase limits the rate of cell growth-linked de novo purine biosynthesis in the presence of constant capacity of salvage purine biosynthesis","volume":"272","author":"Yamaoka","year":"1997","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5171","DOI":"10.1128\/JB.183.17.5171-5179.2001","article-title":"Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureus","volume":"183","author":"Bischoff","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"6278","DOI":"10.1128\/JB.185.21.6278-6286.2003","article-title":"Molecular architecture of the regulatory locus sae of Staphylococcus aureus and its impact on expression of virulence factors","volume":"185","author":"Steinhuber","year":"2003","journal-title":"J. Bacteriol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"16051","DOI":"10.1038\/nmicrobiol.2016.51","article-title":"Persister formation in Staphylococcus aureus is associated with ATP depletion","volume":"1","author":"Conlon","year":"2016","journal-title":"Nat. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1111\/j.1469-0691.2008.02035.x","article-title":"Activities of antistaphylococcal antibiotics towards the extracellular and intraphagocytic forms of Staphylococcus aureus isolates from a patient with persistent bacteraemia and endocarditis","volume":"14","author":"Lemaire","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1128\/CMR.00042-09","article-title":"Reduced vancomycin susceptibility in Staphylococcus aureus, including vancomycin-intermediate and heterogeneous vancomycin-intermediate strains: resistance mechanisms, laboratory detection, and clinical implications","volume":"23","author":"Howden","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1093\/jac\/dkq292","article-title":"Pharmacodynamic effect of clinical vancomycin exposures on cell wall thickness in heterogeneous vancomycin-intermediate Staphylococcus aureus","volume":"65","author":"Rose","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_43","first-page":"207","article-title":"Staphylococcal biofilms","volume":"322","author":"Otto","year":"2008","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1093\/infdis\/jiu007","article-title":"Impact of vancomycin on sarA-mediated biofilm formation: role in persistent endovascular infections due to methicillin-resistant Staphylococcus aureus","volume":"209","author":"Abdelhady","year":"2014","journal-title":"J. Infect. Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1038\/nri1180","article-title":"Defensins: Antimicrobial peptides of innate immunity","volume":"3","author":"Ganz","year":"2003","journal-title":"Nat. Rev. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1172\/JCI562","article-title":"Platelet microbicidal proteins and neutrophil defensin disrupt the Staphylococcus aureus cytoplasmic membrane by distinct mechanisms of action","volume":"101","author":"Yeaman","year":"1998","journal-title":"J. Clin. Investig."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Cole, J.N., and Nizet, V. (2016). Bacterial evasion of host antimicrobial peptide defenses. Microbiol. Spectr., 4.","DOI":"10.1128\/9781555819286.ch15"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1086\/315063","article-title":"Influence of in vitro susceptibility phenotype against thrombin-induced platelet microbicidal protein on treatment and prophylaxis outcomes of experimental Staphylococcus aureus endocarditis","volume":"180","author":"Dhawan","year":"1999","journal-title":"J. Infect. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1084\/jem.193.9.1067","article-title":"Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with l-lysine","volume":"193","author":"Peschel","year":"2001","journal-title":"J. Exp. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3311","DOI":"10.1128\/IAI.00255-15","article-title":"Persistent Staphylococcus aureus isolates from two independent cases of bacteremia display increased bacterial fitness and novel immune evasion phenotypes","volume":"83","author":"Richards","year":"2015","journal-title":"Infect. Immun."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/nri1785","article-title":"Neutrophils and immunity: Challenges and opportunities","volume":"6","author":"Nathan","year":"2006","journal-title":"Nat. Rev. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Geiger, T., Francois, P., Liebeke, M., Fraunholz, M., Goerke, C., Krismer, B., Schrenzel, J., Lalk, M., and Wolz, C. (2012). The stringent response of Staphylococcus aureus and its impact on survival after phagocytosis through the induction of intracellular PSMs expression. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1003016"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1111\/1574-6976.12057","article-title":"Phenol-soluble modulins--critical determinants of staphylococcal virulence","volume":"38","author":"Cheung","year":"2014","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s00441-008-0687-4","article-title":"The role of endothelial cell biology in endocarditis","volume":"335","author":"Chorianopoulos","year":"2009","journal-title":"Cell Tissue Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3824","DOI":"10.1128\/IAI.00405-08","article-title":"The fibrinogen- and fibronectin-binding domains of Staphylococcus aureus fibronectin-binding protein A synergistically promote endothelial invasion and experimental endocarditis","volume":"76","author":"Piroth","year":"2008","journal-title":"Infect. Immun."},{"key":"ref_56","first-page":"302","article-title":"Variant subpopulations of Staphylococcus aureus as cause of persistent and recurrent infections","volume":"3","author":"Proctor","year":"1994","journal-title":"Infect. Agents Dis."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.1038\/emi.2014.3","article-title":"Persisters, persistent infections and the Yin-Yang model","volume":"3","author":"Zhang","year":"2014","journal-title":"Emerg. Microbes. Infect."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.tim.2008.11.005","article-title":"Staphylococcus aureus: New evidence for intracellular persistence","volume":"17","author":"Garzoni","year":"2009","journal-title":"Trends Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1128\/CMR.13.1.16","article-title":"Exotoxins of Staphylococcus aureus","volume":"13","author":"Dinges","year":"2000","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1093\/jac\/dkv368","article-title":"When sepsis persists: A review of MRSA bacteraemia salvage therapy","volume":"71","author":"Kullar","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.jinf.2012.08.007","article-title":"Efficacy of linezolid-based salvage therapy compared with glycopeptide-based therapy in patients with persistent methicillin-resistant Staphylococcus aureus bacteremia","volume":"65","author":"Park","year":"2012","journal-title":"J. Infect."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"379","DOI":"10.3109\/00365548.2014.995696","article-title":"Telavancin for refractory methicillin-resistant Staphylococcus aureus bacteremia and infective endocarditis","volume":"47","author":"Ruggero","year":"2015","journal-title":"Infect. Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4497","DOI":"10.1128\/AAC.00386-15","article-title":"Evaluation of ceftaroline alone and in combination against biofilm-producing methicillin-resistant Staphylococcus aureus with reduced susceptibility to daptomycin and vancomycin in an in vitro pharmacokinetic\/pharmacodynamic model","volume":"59","author":"Barber","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"5790","DOI":"10.1128\/JB.187.16.5790-5798.2005","article-title":"SarA positively controls bap-dependent biofilm formation in Staphylococcus aureus","volume":"187","author":"Trotonda","year":"2005","journal-title":"J. Bacteriol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1093\/infdis\/jiw386","article-title":"The global regulon sarA regulates beta-lactam antibiotic resistance in methicillin-resistant Staphylococcus aureus in vitro and in endovascular infections","volume":"214","author":"Li","year":"2016","journal-title":"J. Infect. Dis."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"416","DOI":"10.3389\/fmicb.2015.00416","article-title":"SarA based novel therapeutic candidate against Staphylococcus aureus associated with vascular graft infections","volume":"6","author":"Arya","year":"2015","journal-title":"Front. Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/8\/2\/71\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:54:28Z","timestamp":1760187268000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/8\/2\/71"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,29]]},"references-count":66,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,6]]}},"alternative-id":["antibiotics8020071"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics8020071","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,29]]}}}