{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T03:20:21Z","timestamp":1764904821089,"version":"build-2065373602"},"reference-count":14,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T00:00:00Z","timestamp":1568160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Economy, Industry, and Competitiveness","award":["AGL2016-74882-C3"],"award-info":[{"award-number":["AGL2016-74882-C3"]}]},{"name":"Autonomous Community of Madrid","award":["S2013\/ABI-2747"],"award-info":[{"award-number":["S2013\/ABI-2747"]}]},{"name":"Spanish Ministry of Science, Innovation and Universities","award":["BES-2017-080264"],"award-info":[{"award-number":["BES-2017-080264"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Carbapenems are considered last-resort antimicrobials, especially for treating infections involving multidrug-resistant Gram-negative bacteria. In recent years, extended-spectrum \u03b2-lactamase (ESBL) and carbapenemase-producing Gram-negative bacteria have become widespread in hospitals, community settings, and the environment, reducing the range of effective therapeutic alternatives. The use of colistin to treat infection caused by these multi-drug bacteria may favour the selection and persistence of carbapenem-resistant bacteria. In this study, it is described, for the first time to our knowledge, a carbapenemase-producing isolate of Elizabethkingia meningoseptica from healthy pigs in Spain. The isolate we report was recovered during a study to detect colistin-resistant bacteria from faecal samples of healthy food-production animals using a chromogenic selective medium. Unexpectedly, we found an isolate of Elizabethkingia meningoseptica with high Minimum Inhibitory Concentration (MIC) values for several antibiotics tested. Molecular analysis did not show any mcr family genes related with colistin resistance, but two carbapenemase genes, blaB-12_1 and blaGOB-17_1, were detected. This finding in healthy animals could suggest that colistin may favour the selection and persistence of carbapenem-resistant bacteria.<\/jats:p>","DOI":"10.3390\/antibiotics8030146","type":"journal-article","created":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T11:26:34Z","timestamp":1568201194000},"page":"146","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Carbapenemase-Producing Elizabethkingia Meningoseptica from Healthy Pigs Associated with Colistin Use in Spain"],"prefix":"10.3390","volume":"8","author":[{"given":"Pedro","family":"Miguela-Villoldo","sequence":"first","affiliation":[{"name":"VISAVET Health Surveillance Centre, Universidad Complutense, Madrid 28040, Spain"},{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular y Microbiolog\u00eda, Instituto Tecnol\u00f3gico Agrario de Castilla y Le\u00f3n, 47071 Valladolid, Spain"},{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biotecnolog\u00eda y Ciencia de los Alimentos, Universidad de Burgos, 09001 Burgos, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5357-3684","authenticated-orcid":false,"given":"Miguel \u00c1.","family":"Moreno","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Universidad Complutense, Madrid 28040, Spain"},{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8795-854X","authenticated-orcid":false,"given":"David","family":"Rodr\u00edguez-L\u00e1zaro","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biotecnolog\u00eda y Ciencia de los Alimentos, Universidad de Burgos, 09001 Burgos, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Quesada","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Veterinaria, Universidad de Extremadura, 10003 C\u00e1ceres, Spain"},{"name":"INBIO G+C, Universidad de Extremadura, 10003 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Universidad Complutense, Madrid 28040, Spain"},{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4509-6021","authenticated-orcid":false,"given":"Mar\u00eda","family":"Ugarte-Ruiz","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Universidad Complutense, Madrid 28040, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2299","DOI":"10.3389\/fmicb.2018.02299","article-title":"Prevalence and Emergence of Extended-Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram Negative Bacteria of Animal Origin in the Mediterranean Basin","volume":"9","author":"Dandachi","year":"2018","journal-title":"Front. 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Agents Chemother."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"215237","DOI":"10.1155\/2011\/215237","article-title":"Elizabethkingia meningosepticum (Chryseobacterium meningosepticum) Infections in Children","volume":"2011","author":"Ceyhan","year":"2011","journal-title":"Int. J. Pediatr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.rvsc.2019.01.010","article-title":"National colistin sales versus colistin resistance in Spanish pig production","volume":"123","author":"Moreno","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e000002","DOI":"10.1136\/vropen-2013-000002","article-title":"Survey of quantitative antimicrobial consumption per production stage in farrow-to-finish pig farms in Spain","volume":"1","author":"Moreno","year":"2014","journal-title":"Vet. Rec. Open"},{"key":"ref_8","unstructured":"European Medicines Agency (2016). 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Chemother."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing","volume":"58","author":"Carattoli","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ijantimicag.2018.03.022","article-title":"Co-occurrence of mcr-1, mcr-4 and mcr-5 genes in multidrug-resistant ST10 Enterotoxigenic and Shiga toxin-producing Escherichia coli in Spain (2006\u20132017)","volume":"52","author":"Garcia","year":"2018","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.diagmicrobio.2018.08.014","article-title":"Emergence and dissemination of colistin-resistant Klebsiella pneumoniae isolates expressing OXA-48 plus CTX-M-15 in patients not previously treated with colistin in a Spanish university hospital","volume":"93","author":"Tedim","year":"2019","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.ijantimicag.2017.03.014","article-title":"First report of an Escherichia coli strain from swine carrying an OXA-181 carbapenemase and the colistin resistance determinant MCR-1","volume":"50","author":"Pulss","year":"2017","journal-title":"Int. J. Antimicrob. Agents"}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/8\/3\/146\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:18:49Z","timestamp":1760188729000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/8\/3\/146"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,11]]},"references-count":14,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,9]]}},"alternative-id":["antibiotics8030146"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics8030146","relation":{},"ISSN":["2079-6382"],"issn-type":[{"type":"electronic","value":"2079-6382"}],"subject":[],"published":{"date-parts":[[2019,9,11]]}}}