{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T21:23:13Z","timestamp":1783459393508,"version":"3.55.0"},"reference-count":36,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,16]],"date-time":"2020-01-16T00:00:00Z","timestamp":1579132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Antimicrobial resistance is one of the most important public health issues. Besides classical multidrug resistance species associated with medical care involved in superficial or invasive infections, there are strains less commonly associated with hospital or outpatient setting\u2019s infections. Non-diphtheria Corynebacterium spp. could produce infections in patients with or without immune-compromised status. The aim of our study was to determine the susceptibility to antimicrobial agents to Corynebacterium spp. from clinical samples collected from Romanian hospitalized individuals and outpatients. Twenty Corynebacterium strains were isolated and identified as Corynebacterium striatum (n = 7), Corynebacterium amycolatum (n = 7), C. urealyticum (n = 3), Corynebacterium afermentans (n = 2), and Corynebacterium pseudodiphtheriticum (n = 1). All isolates have been tested for antibiotic susceptibility by standardized disc diffusion method and minimal inhibitory concentration (MIC) tests. Seventeen isolates demonstrated multidrug resistance phenotypes. The molecular support responsible for high resistance to quinolones for ten of these strains was determined by the detection of point mutation in the gene sequence gyrA.<\/jats:p>","DOI":"10.3390\/antibiotics9010031","type":"journal-article","created":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T04:14:41Z","timestamp":1579234481000},"page":"31","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Antimicrobial Susceptibility Testing for Corynebacterium Species Isolated from Clinical Samples in Romania"],"prefix":"10.3390","volume":"9","author":[{"given":"Cristiana Cerasella","family":"Dragomirescu","sequence":"first","affiliation":[{"name":"\u201cCantacuzino\u201d National Medico Military Institute for Research and Development, 050096 Bucharest, Romania"},{"name":"\u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandusa Elena","family":"Lixandru","sequence":"additional","affiliation":[{"name":"\u201cCantacuzino\u201d National Medico Military Institute for Research and Development, 050096 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ileana Luminita","family":"Coldea","sequence":"additional","affiliation":[{"name":"\u201cCantacuzino\u201d National Medico Military Institute for Research and Development, 050096 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olguta Nicoleta","family":"Corneli","sequence":"additional","affiliation":[{"name":"\u201cCantacuzino\u201d National Medico Military Institute for Research and Development, 050096 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Pana","sequence":"additional","affiliation":[{"name":"\u201cCantacuzino\u201d National Medico Military Institute for Research and Development, 050096 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andi Marian","family":"Palade","sequence":"additional","affiliation":[{"name":"\u201cCantacuzino\u201d National Medico Military Institute for Research and Development, 050096 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Violeta Corina","family":"Cristea","sequence":"additional","affiliation":[{"name":"Central Reference Laboratory Synevo, 021408 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana","family":"Suciu","sequence":"additional","affiliation":[{"name":"BEIA Consult International, Peroni 16, 041386 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Suciu","sequence":"additional","affiliation":[{"name":"BEIA Consult International, Peroni 16, 041386 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8694-6195","authenticated-orcid":false,"given":"Loredana Sabina Cornelia","family":"Manolescu","sequence":"additional","affiliation":[{"name":"\u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8285-7176","authenticated-orcid":false,"given":"Loredana Gabriela","family":"Popa","sequence":"additional","affiliation":[{"name":"\u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"},{"name":"Colentina Clinical Hospital (CDPC), 020125 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0794-9329","authenticated-orcid":false,"given":"Mircea Ioan","family":"Popa","sequence":"additional","affiliation":[{"name":"\u201cCantacuzino\u201d National Medico Military Institute for Research and Development, 050096 Bucharest, Romania"},{"name":"\u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1111\/lam.12883","article-title":"Contemporary microbiology and identification of Corynebacteria spp. causing infections in human","volume":"66","author":"Zasada","year":"2018","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1128\/CMR.10.1.125","article-title":"Clinical Microbiology of Coryneform Bacteria","volume":"10","author":"Funke","year":"1997","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1099\/jmm.0.2008\/003343-0","article-title":"Endocartidis due to Corynebacterium amycolatum, Case report","volume":"57","author":"Dalal","year":"2008","journal-title":"J. Med. Microbiol."},{"key":"ref_4","first-page":"47","article-title":"Bacteriemia caused by Corynebacterium amycolatum with a novel mutation in gyrA gene that confers high-level quinolone resistance","volume":"31","author":"Yoon","year":"2011","journal-title":"Korean J. Lab. Med."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Belmares, J., Detterline, S., Pak, J.B., and Parada, J.P. (2007). Corynebacterium endocarditis species-specific risk factors and outcomes. BMC Infect. Dis., 7.","DOI":"10.1186\/1471-2334-7-4"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1128\/AAC.39.1.208","article-title":"Antimicrobial susceptibilities of Corynebacterium species and other non-spore-forming gram-positive bacilli to 18 antimicrobial agents","volume":"39","author":"Soriano","year":"1995","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ramos, J.N., Souza, C., Faria, Y.V., da Silva, E.C., Veras, J.F.C., Baio, P.V.P., Seabra, S.H., de Oliveira Moreira, L., Hirata J\u00fanior, R., and Mattos-Guaraldi, A.L. (2019). Bloodstream and catheter-related infections due to different clones of multidrug-resistant and biofilm producer Corynebacterium striatum. BMC Infect. Dis., 19.","DOI":"10.1186\/s12879-019-4294-7"},{"key":"ref_8","first-page":"e4670","article-title":"Recurrent Bacteremia with Corynebacterium striatum After Prosthetic Valve Replacement: A Case Report","volume":"11","author":"Syed","year":"2019","journal-title":"Cureus"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e00575","DOI":"10.1016\/j.idcr.2019.e00575","article-title":"Corynebacterium striatum bacteremia associated with cellulitis in a patient with cirrhosis","volume":"17","author":"Elkayam","year":"2019","journal-title":"IDCases"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1093\/ndt\/14.4.977","article-title":"Ureteral stenosis secondary encrustation by urea-splitting Corynebacterium urealyticum in a kidney transplant patient","volume":"14","author":"Herrero","year":"1999","journal-title":"Nephrol. Dial. Transplant."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/BF01962333","article-title":"Infections caused by antibiotic-resistant Corynebacterium group D2","volume":"7","author":"Soriano","year":"1988","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/BF01967098","article-title":"Osteomyelitis due to Corynebacterium group D2","volume":"10","author":"Chomarat","year":"1991","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1007\/PL00011297","article-title":"Bacteremia caused by multiply resistant Corynebacterium urealyticum: six case reports and review","volume":"20","author":"Guerra","year":"2001","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e00610","DOI":"10.1016\/j.idcr.2019.e00610","article-title":"Acase of recurrent Corynebacterium jeikeium endocarditis: Unanswered questions for the treatment of chronic endovascular infections","volume":"18","author":"Clarke","year":"2019","journal-title":"IDCases"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1093\/clinids\/22.5.851","article-title":"A Necrotic Soft-Tissue Lesion Due to Corynebacterium urealyticum in a Neutropenic Child","volume":"22","author":"Saavedra","year":"1996","journal-title":"Clin. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1099\/0022-1317-49-8-759","article-title":"Biochemical, antimicrobial susceptibility and genotyping studies on Corynebacterium urealyticum isolates from diverse sources","volume":"49","author":"Nieto","year":"2000","journal-title":"J. Med. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1097\/01.SMJ.0000100114.54554.C1","article-title":"Corynebacterium afermentans Lung Abscess and Empyema in a Patient with Human Immunodeficiency Virus Infect","volume":"97","author":"Minkin","year":"2004","journal-title":"South. Med. J."},{"key":"ref_18","first-page":"594","article-title":"Lung abscess involving Corynebacterium pseudodiphtheriticum in a patient with AIDS-related complex","volume":"86","author":"Andavolu","year":"1986","journal-title":"N. Y. State J. Med."},{"key":"ref_19","first-page":"325","article-title":"Corynebacterium pseudodiphtheriticum pneumonia in an immunocompetent patient","volume":"54","author":"Chiner","year":"1999","journal-title":"Monaldi Arch. Chest. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1093\/clinids\/13.5.887","article-title":"Endocarditis due to Corynebacterium pseudodiphtheriticum: five case reports, review and antibiotic susceptibilities of nine strains","volume":"13","author":"Morris","year":"1991","journal-title":"Rev. Infect. Dis."},{"key":"ref_21","first-page":"12926","article-title":"Comparative analysis of different phenotypic and molecular methods used for the taxonomic identification of Corynebacterium spp. isolated from clinical samples in Romania","volume":"22","author":"Dragomirescu","year":"2017","journal-title":"Rom. Biotechnol. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.3201\/eid2211.160141","article-title":"Multidrug-resistant Corynebacterium striatum associated with increased use of parenteral antimicrobial drugs","volume":"22","author":"Hahn","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.midw.2019.07.018","article-title":"A Romanian experience of syphilis in pregnancy and childbirth","volume":"78","author":"Manolescu","year":"2019","journal-title":"Midwifery"},{"key":"ref_24","first-page":"7817","article-title":"Association of hepatitis B infection in patients with HIV Encephalopathy","volume":"17","author":"Marinescu","year":"2012","journal-title":"Rom. Biotechnol. Lett."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jiph.2014.05.006","article-title":"Nontoxigenic penicillin-resistant cutaneous C. diphtheriae infection: a case report and review of the literature","volume":"8","author":"FitzGerald","year":"2015","journal-title":"J. Infect. Public Health"},{"key":"ref_26","first-page":"DC19","article-title":"Non Diphtheritic Corynebacteria: An Emerging Nosocomial Pathogen in Skin and Soft Tissue Infection","volume":"9","author":"Rudresh","year":"2015","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1007\/BF01709376","article-title":"Sepsis due to multiply resistant Corynebacteriu mamycolatum","volume":"15","year":"1996","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"9704","DOI":"10.1038\/s41598-017-10081-y","article-title":"Occurrence of Corynebacterium striatum as an emerging antibioticresistant nosocomial pathogen in a Tunisian hospital","volume":"7","author":"Alibi","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_29","first-page":"47","article-title":"Corynebacterium urealyticum in urinary infections in children","volume":"43","author":"Coman","year":"1998","journal-title":"Bacteriol. Virusol. Parazitol. Epidemiol."},{"key":"ref_30","first-page":"37","article-title":"Empyema caused by Corynebacterium striatum","volume":"47","author":"Diculencu","year":"1998","journal-title":"Pneumoftiziologia"},{"key":"ref_31","first-page":"15","article-title":"Etiology of ventilator-associated pneumonia at the Cardiovascular Surgery Clinic of Ia\u015fi","volume":"116","author":"Serban","year":"2012","journal-title":"Rev. Med. Chir. Soc. Med. Nat. Iasi"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1128\/AAC.49.5.1714-1719.2005","article-title":"Relationship between Mutations in the gyrA Gene and Quinolone Resistance in Clinical Isolates of Corynebacterium striatum and Corynebacterium amycolatum","volume":"49","author":"Sierra","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_33","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2012). Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. Propos. Guidel. M45-A2, 30, 18."},{"key":"ref_34","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2006). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, CLSI. [7th ed.]. Approv. Stand. M7-A7."},{"key":"ref_35","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (2019, October 16). Breakpoint tables for interpretation of MICs and zone diameters. Available online: http:\/\/www.eucast.org\/fileadmin\/src\/media\/PDFs\/EUCAST_files\/Breakpoint_tables\/Breakpoint_table_v_4.0.pdf."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s10916-015-0327-y","article-title":"Big data, internet of things and cloud convergence\u2013an architecture for secure e-health applications","volume":"39","author":"Suciu","year":"2015","journal-title":"J. Med. Syst."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/9\/1\/31\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:19:52Z","timestamp":1760361592000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/9\/1\/31"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,16]]},"references-count":36,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["antibiotics9010031"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics9010031","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,16]]}}}