{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T08:17:19Z","timestamp":1778833039202,"version":"3.51.4"},"reference-count":28,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T00:00:00Z","timestamp":1581552000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Program on Key Research Project of China","award":["SQ2019YFE010999; 2017YFC1600103; 2018YFD0500501"],"award-info":[{"award-number":["SQ2019YFE010999; 2017YFC1600103; 2018YFD0500501"]}]},{"name":"European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement","award":["861917"],"award-info":[{"award-number":["861917"]}]},{"name":"SAFFI and Zhejiang Provincial Natural Science Foundation of China","award":["LR19C180001"],"award-info":[{"award-number":["LR19C180001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Colistin is considered to be a \u2018last-resort\u2019 antimicrobial for the treatment of multidrug-resistant Gram-negative bacterial infections. Identification of Enterobacteriaceae, carrying the transferable colistin resistance gene mcr-1, has recently provoked a global health concern. This report presents the first detection of a hydrogen sulfide (H2S)-producing Escherichia coli variant isolated from a human in China, with multidrug resistance (MDR) properties, including colistin resistance by the mcr-1 gene, which could have great implications for the treatment of human infections.<\/jats:p>","DOI":"10.3390\/antibiotics9020080","type":"journal-article","created":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T10:09:57Z","timestamp":1581674997000},"page":"80","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Genomic Characterization of New Variant of Hydrogen Sulfide (H2S)-Producing Escherichia coli with Multidrug Resistance Properties Carrying the mcr-1 Gene in China"],"prefix":"10.3390","volume":"9","author":[{"given":"Silpak","family":"Biswas","sequence":"first","affiliation":[{"name":"Institute of Preventive Veterinary Sciences &amp; Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou 310007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3834-8523","authenticated-orcid":false,"given":"Mohammed","family":"Elbediwi","sequence":"additional","affiliation":[{"name":"Institute of Preventive Veterinary Sciences &amp; Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou 310007, China"},{"name":"Animal Health Research Institute, Agriculture Research Centre, Cairo 11435, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guimin","family":"Gu","sequence":"additional","affiliation":[{"name":"Guangxi Center for Disease Prevention and Control, Nanning 530000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6787-0794","authenticated-orcid":false,"given":"Min","family":"Yue","sequence":"additional","affiliation":[{"name":"Institute of Preventive Veterinary Sciences &amp; Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou 310007, China"},{"name":"Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou 310007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1099\/jmm.0.022475-0","article-title":"Commensal Escherichia coli of healthy humans: A reservoir for antibiotic-resistance determinants","volume":"59","author":"Bailey","year":"2010","journal-title":"J. Med. Microbiol."},{"key":"ref_2","first-page":"641","article-title":"Hydrogen sulfide producing variants of Escherichia coli","volume":"79","author":"Lautrop","year":"1971","journal-title":"Acta Pathol. Microbiol. Scand."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1128\/AAC.14.5.765","article-title":"Genetic and molecular characterization of an Escherichia coli plasmid coding for hydrogen sulfide production and drug resistance","volume":"14","author":"Jones","year":"1978","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1126\/science.1209855","article-title":"H2S: A Universal Defense Against Antibiotics in Bacteria","volume":"334","author":"Shatalin","year":"2011","journal-title":"Science"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1021\/acsinfecdis.7b00090","article-title":"Hydrogen Sulfide and Reactive Sulfur Species Impact Proteome S-Sulfhydration and Global Virulence Regulation in Staphylococcus aureus","volume":"3","author":"Peng","year":"2017","journal-title":"ACS Infect. Dis."},{"key":"ref_6","unstructured":"Quinn, P.J., Markey, B.K., Leonard, F.C., Hartigan, P., Fanning, S., and Fitzpatrick, E. (2002). Veterinary Microbiology and Microbial Disease: Pathogenic Bacteria, Blackwell Scientific."},{"key":"ref_7","unstructured":"Clinical and Laboratory Standards Institute (2019). Performance Standards for Antimicrobial Susceptibility Testing, CL SI. [29th ed.]."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Elbediwi, M., Li, Y., Paudyal, N., Pan, H., Li, X., Xie, S., Rajkovic, A., Feng, Y., Fang, W., and Rankin, S.C. (2019). Global Burden of Colistin-Resistant Bacteria: Mobilized Colistin Resistance Genes Study (1980\u20132018). Microorganisms, 7.","DOI":"10.3390\/microorganisms7100461"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0732-8893(96)00027-2","article-title":"Thermosensitive transfer of antimicrobial resistances and citrate utilization and cotransfer of hydrogen sulfide production from an Escherichia coli isolate","volume":"24","author":"Harnett","year":"1996","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1099\/jmm.0.053645-0","article-title":"New integron gene arrays from multiresistant clinical isolates of members of the Enterobacteriaceae and Pseudomonas aeruginosa from hospitals in Malaysia","volume":"62","author":"Kor","year":"2013","journal-title":"J. Med. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1473-3099(15)00424-7","article-title":"Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: A microbiological and molecular biological study","volume":"16","author":"Liu","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1586\/eri.12.78","article-title":"Colistin: An update on the antibiotic of the 21st century","volume":"10","author":"Biswas","year":"2012","journal-title":"Expert Rev. Anti Infect. Ther."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1016\/j.tim.2018.02.006","article-title":"Towards Understanding MCR-like Colistin Resistance","volume":"26","author":"Sun","year":"2018","journal-title":"Trends Microbiol."},{"key":"ref_14","first-page":"187","article-title":"First three Escherichia coli isolates harboring mcr-1 in Uruguay","volume":"20","author":"Vieytes","year":"2019","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1128\/AAC.01574-08","article-title":"oqxAB encoding a multidrug efflux pump in human clinical isolates of Enterobacteriaceae","volume":"53","author":"Kim","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.jgar.2018.11.003","article-title":"Frequency of DNA gyrase and topoisomerase IV mutations and plasmid-mediated quinolone resistance genes among Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in Azerbaijan, Iran","volume":"17","author":"Azargun","year":"2019","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1093\/jac\/dkl020","article-title":"The role of horizontal gene transfer in the spread of trimethoprim-sulfamethoxazole resistance among uropathogenic Escherichia coli in Europe and Canada","volume":"57","author":"Blahna","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1111\/j.1472-765X.2009.02717.x","article-title":"Distribution of integron-associated trimethoprim-sulfamethoxazole resistance determinants among Escherichia coli from humans and food-producing animals","volume":"49","author":"Ho","year":"2009","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.plasmid.2016.02.002","article-title":"pCERC3 from a commensal ST95 Escherichia coli: A ColV virulence-multiresistance plasmid carrying a sul3-associated class 1 integron","volume":"84\u201385","author":"Moran","year":"2016","journal-title":"Plasmid"},{"key":"ref_20","first-page":"210","article-title":"\u03b2-lactamases produced by amoxicillin-clavulanate-resistant enterobacteria isolated in BuenosAires, Argentina: A new blaTEM gene","volume":"46","author":"Badaracco","year":"2014","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1089\/mdr.2015.0354","article-title":"Detection of CTX-M-15-Producing Escherichia coli Isolates of Lineages ST131-B2 and ST167-A in Environmental Samples of a Tunisian Hospital","volume":"22","author":"Dziri","year":"2016","journal-title":"Microb. Drug Resist."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1099\/jmm.0.000777","article-title":"Epidemic spread of OXA-48 beta-lactamase in Croatia","volume":"67","author":"Slade","year":"2018","journal-title":"J. Med. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1093\/jac\/dkg473","article-title":"Sulphonamide resistance gene sul3 found in Escherichia coli isolates from human sources","volume":"52","author":"Grape","year":"2003","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.ijantimicag.2010.01.012","article-title":"Genetic environment of sul genes and characterisation of integrons in Escherichia coli isolates of blood origin in a Spanish hospital","volume":"35","author":"Olarte","year":"2010","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.ijantimicag.2004.12.004","article-title":"Acquired sulphonamide resistance genes in faecal Escherichia coli from healthy children in Bolivia and Peru","volume":"25","author":"Infante","year":"2005","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1089\/mdr.2015.0045","article-title":"Antibiotic Resistance, Virulence, and Genetic Background of Community-Acquired Uropathogenic Escherichia coli from Algeria","volume":"21","author":"Yahiaoui","year":"2015","journal-title":"Microb. Drug Resist."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1556\/EuJMI.3.2013.2.7","article-title":"Prevalence of tet genes mediating tetracycline resistance in Escherichia coli clinical isolates in OsunState, Nigeria","volume":"3","author":"Olowe","year":"2013","journal-title":"Eur. J. Microbiol. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1099\/jmm.0.015008-0","article-title":"Prevalence of trimethoprim resistance genes in Escherichia coli isolates of human and animal origin in Lithuania","volume":"59","author":"Povilonis","year":"2010","journal-title":"J. Med. Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/9\/2\/80\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:57:24Z","timestamp":1760173044000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/9\/2\/80"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,13]]},"references-count":28,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["antibiotics9020080"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics9020080","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,13]]}}}