{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:50:06Z","timestamp":1760237406919,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T00:00:00Z","timestamp":1589241600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003032","name":"Association Nationale de la Recherche et de la Technologie","doi-asserted-by":"publisher","award":["2018\/0659"],"award-info":[{"award-number":["2018\/0659"]}],"id":[{"id":"10.13039\/501100003032","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The evaluation of antibacterial activity of metal surfaces can be carried out using various published guidelines which do not always agree with each other on technical conditions and result interpretation. Moreover, these technical conditions are sometimes remote from real-life ones, especially those found in health-care facilities, and do not include a variety of antibiotic-resistant strains. A worst-case scenario protocol adapted from published guidelines was validated on two reference strains (Staphylococcus aureus ATCC 6538 and Enterobacter aerogenes ATCC 13048). This protocol was designed to be as close as possible to a healthcare facility environment, including a much shorter exposure-time than the one recommended in guidelines, and evaluated the impact of parameters such as the method used to prepare inocula, seed on the surface, and recover bacteria following exposure. It was applied to a panel of 12 antibiotic-resistant strains (methicillin resistant, vancomycin-resistant, beta-lactamase, and carbapenemase producing strains as well as efflux pump-overexpressing ones) chosen as representative of the main bacteria causing hospital acquired infections. Within a 5-min exposure time, the tested brass surface displayed an antibacterial effect meeting a reduction cut-off of 99% compared to stainless steel, whatever the resistance mechanism harbored by the bacteria.<\/jats:p>","DOI":"10.3390\/antibiotics9050245","type":"journal-article","created":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T10:53:55Z","timestamp":1589280835000},"page":"245","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Validation of a Worst-Case Scenario Method Adapted to the Healthcare Environment for Testing the Antibacterial Effect of Brass Surfaces and Implementation on Hospital Antibiotic-Resistant Strains"],"prefix":"10.3390","volume":"9","author":[{"given":"Emilie","family":"Dauvergne","sequence":"first","affiliation":[{"name":"Laboratoire AGIR - UR UPJV 4294, UFR de Pharmacie, Universit\u00e9 de Picardie Jules Verne, 80037 Amiens, France"},{"name":"FAVI Limited Company, 80490 Hallencourt, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinne","family":"Lacquemant","sequence":"additional","affiliation":[{"name":"FAVI Limited Company, 80490 Hallencourt, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Crespin","family":"Adjid\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire Hygi\u00e8ne, Risque Biologique et Environnement, CHU Amiens-Picardie, 80025 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8986-9666","authenticated-orcid":false,"given":"Catherine","family":"Mulli\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire AGIR - UR UPJV 4294, UFR de Pharmacie, Universit\u00e9 de Picardie Jules Verne, 80037 Amiens, France"},{"name":"Laboratoire Hygi\u00e8ne, Risque Biologique et Environnement, CHU Amiens-Picardie, 80025 Amiens, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,12]]},"reference":[{"key":"ref_1","unstructured":"Centers for Disease Control and Prevention (2020, March 25). 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