{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:30:34Z","timestamp":1777415434002,"version":"3.51.4"},"reference-count":35,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T00:00:00Z","timestamp":1590364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Faculty of Science, Naresuan University","award":["R2562E041"],"award-info":[{"award-number":["R2562E041"]}]},{"name":"PhD scholarship funding program from Science Achievement Scholarship of Thailand (SAST)","award":["student no. 59031494"],"award-info":[{"award-number":["student no. 59031494"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Propionibacterium acnes plays an important role in the pathophysiology of acne vulgaris, the most common chronic inflammatory skin disease of the pilosebaceous unit. This study was conducted to investigate whether the entomopathogenic fungus Polycephalomyces phaothaiensis components have antibacterial and anti-inflammatory effects against P. acnes that may serve for acne treatment. A chemical study by spectroscopic analysis resulted in the identification of seven known compounds. The anti-P. acnes potency of extracts and test compounds was determined by both agar diffusion and broth dilution methods. The ethyl acetate extract from culture broth along with cordytropolone (1) and stipitalide (2) exhibited strong anti- P. acnes activity while (+)-piliformic acid (3) showed weak inhibitory activity. The anti-inflammatory effect of ethyl acetate extract and 1\u20133 was then examined by the quantification of pro-inflammatory cytokines IL-1\u03b2, IL-6, and TNF-\u03b1 on heat-killed P. acnes induced cytokine production by THP-1 cells. The result demonstrated that the extract and its constituents (1\u20133) showed a potent significant effect by inhibiting the P. acnes-induced pro-inflammatory cytokines production in THP-1. Our results suggest for the first time that P. phaothaiensis and its constituents (1 and 2) hold therapeutic value for further studies as a new alternative treatment for acne.<\/jats:p>","DOI":"10.3390\/antibiotics9050274","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T06:43:40Z","timestamp":1590389020000},"page":"274","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3059-8149","authenticated-orcid":false,"given":"Witsanu","family":"Sonyot","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5504-3433","authenticated-orcid":false,"given":"Supaporn","family":"Lamlertthon","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Fungal Research, Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6801-2145","authenticated-orcid":false,"given":"Janet Jennifer","family":"Luangsa-ard","sequence":"additional","affiliation":[{"name":"Plant Microbe Interaction Research Team, BIOTEC, 113 Thailand Science Park, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suchada","family":"Mongkolsamrit","sequence":"additional","affiliation":[{"name":"Plant Microbe Interaction Research Team, BIOTEC, 113 Thailand Science Park, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanchana","family":"Usuwanthim","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Immunology Research Unit, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4415-8489","authenticated-orcid":false,"given":"Kornkanok","family":"Ingkaninan","sequence":"additional","affiliation":[{"name":"Bioscreening Unit, Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand"},{"name":"Faculty of Pharmacy, Airlangga University, Surabaya 60286, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neti","family":"Waranuch","sequence":"additional","affiliation":[{"name":"Cosmetics and Natural Products Research Center, Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3767-8387","authenticated-orcid":false,"given":"Nungruthai","family":"Suphrom","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand"},{"name":"Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1111\/bjd.16248","article-title":"Prevalence of skin disease in a population-based sample of adults from five European countries","volume":"178","author":"Svensson","year":"2018","journal-title":"Br. 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