{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T05:01:54Z","timestamp":1792386114686,"version":"4.4.0"},"reference-count":38,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T00:00:00Z","timestamp":1594598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["D16\/0016\/0007"],"award-info":[{"award-number":["D16\/0016\/0007"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["RD16\/0016\/0008"],"award-info":[{"award-number":["RD16\/0016\/0008"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Corynebacterium urealyticum is a non-diphtherial urease-producing clinically relevant corynebacterial, most frequently involved in urinary tract infections. Most of the C. urealyticum clinical isolates are frequently resistant to several antibiotics. We investigated the susceptibility of 40 C. urealyticum isolated in our institution during the period 2005\u20132017 to eight compounds representative of the main clinically relevant classes of antimicrobial agents. Antimicrobial susceptibility was determined by the Epsilometer test. Resistance genes were searched by PCR. All strains were susceptible to vancomycin whereas linezolid and rifampicin also showed good activity (MICs90 = 1 and 0.4 mg\/L, respectively). Almost all isolates (39\/40, 97.5%) were multidrug resistant. The highest resistance rate was observed for ampicillin (100%), followed by erythromycin (95%) and levofloxacin (95%). Ampicillin resistance was associated with the presence of the blaA gene, encoding a class A \u03b2-lactamase. The two rifampicin-resistant strains showed point mutations driving amino acid replacements in conserved residues of RNA polymerase subunit \u03b2 (RpoB). Tetracycline resistance was due to an efflux-mediated mechanism. Thirty-nine PFGE patterns were identified among the 40 C. urealyticum, indicating that they were not clonally related, but producing sporadic infections. These findings raise the need of maintaining surveillance strategies among this multidrug resistant pathogen.<\/jats:p>","DOI":"10.3390\/antibiotics9070404","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T04:46:01Z","timestamp":1594701961000},"page":"404","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Antimicrobial Susceptibility and Characterization of Resistance Mechanisms of Corynebacterium urealyticum Clinical Isolates"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7878-4499","authenticated-orcid":false,"given":"Itziar","family":"Chapartegui-Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Grupo BIOMEDAGE, Facultad de Medicina, Universidad de Cantabria, Herrera Oria 2, 39011 Santander, Spain"},{"name":"Instituto de Investigaci\u00f3n Valdecilla (IDIVAL), Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Fern\u00e1ndez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Valdecilla (IDIVAL), Herrera Oria s\/n, 39011 Santander, Spain"},{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Marqu\u00e9s de Valdecilla (HUMV), Av. Valdecilla, 25, 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Rodr\u00edguez-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Marqu\u00e9s de Valdecilla (HUMV), Av. Valdecilla, 25, 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo J. P.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias da Sa\u00fade, Universidade Federal da Bahia, Av. Reitor Miguel Calmon, s\/n \u2014Canela, Salvador 40231-300, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8143-5756","authenticated-orcid":false,"given":"Eric R. G. R.","family":"Aguiar","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Center of Biotechnology and Genetics, State University of Santa Cruz (UESC), Ilh\u00e9us 45662-90, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis G. C.","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias da Sa\u00fade, Universidade Federal da Bahia, Av. Reitor Miguel Calmon, s\/n \u2014Canela, Salvador 40231-300, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8795-519X","authenticated-orcid":false,"given":"Jos\u00e9","family":"Ramos-Vivas","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Valdecilla (IDIVAL), Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Calvo","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Valdecilla (IDIVAL), Herrera Oria s\/n, 39011 Santander, Spain"},{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Marqu\u00e9s de Valdecilla (HUMV), Av. Valdecilla, 25, 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6091-4045","authenticated-orcid":false,"given":"Luis","family":"Mart\u00ednez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Unidad de Gesti\u00f3n Cl\u00ednica, Hospital Universitario Reina Sof\u00eda, Av. Men\u00e9ndez Pidal, s\/n, 14004 C\u00f3rdoba, Spain"},{"name":"Departamento de Microbiolog\u00eda, Universidad de C\u00f3rdoba, Campus Rabanales. Edif. Severo Ochoa (C6), 14071 C\u00f3rdoba, Spain"},{"name":"Instituto Maim\u00f3nides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC), Av. Men\u00e9ndez Pidal, s\/n, 14004 C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5735-4668","authenticated-orcid":false,"given":"Jes\u00fas","family":"Navas","sequence":"additional","affiliation":[{"name":"Grupo BIOMEDAGE, Facultad de Medicina, Universidad de Cantabria, Herrera Oria 2, 39011 Santander, Spain"},{"name":"Instituto de Investigaci\u00f3n Valdecilla (IDIVAL), Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.1128\/JCM.00796-12","article-title":"The genus Corynebacterium and other medically relevant coryneform-like bacteria","volume":"50","author":"Bernard","year":"2012","journal-title":"J. Clin. 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