{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T01:52:13Z","timestamp":1790214733806,"version":"4.1.0"},"reference-count":28,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T00:00:00Z","timestamp":1598227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013414","name":"Conseil R\u00e9gional des Pays de la Loire","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/501100013414","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CODIFAB","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The present investigation aimed to utilize a direct wood disc diffusion method to study the influence of plane of cutting, cutting method, sterilization method, and origin of tree on the antimicrobial activity of wood material. Six oak wood trees (Quercus petraea) were collected from 3 different locations in France. They were cut into 4 mm thick slices with either transverse (RT), tangential (LT) or radial (LR) faces. Round discs (diameter 9.95 \u00b1 0.1 mm) were cut from the slices via a laser machine or a manual punch machine, and were sterilized with gamma irradiation (25 kGy) or autoclaving (121 \u00b0C). The antimicrobial activity of wood was tested using a direct diffusion method against Staphylococcus aureus and Acinetobacter baumannii isolates. The zone of inhibition around the wooden disc was recorded following the recommendations used for antibiotics tests. The results showed that S. aureus was more susceptible than A. baumannii, to the chemicals that diffused from the wood. The transverse face discs exhibited higher antimicrobial activity. Samples that had been sterilized by autoclaving showed significantly (p &lt; 0.05) lower antimicrobial activity, whereas the cutting method and origin of tree did not influence the antimicrobial activity of wood material. Therefore, the choice of sterilization method and cutting planes must be taken into account while studying and interpreting the antibacterial properties of wood material.<\/jats:p>","DOI":"10.3390\/antibiotics9090535","type":"journal-article","created":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T09:24:56Z","timestamp":1598347496000},"page":"535","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Experimental Parameters Influence the Observed Antimicrobial Response of Oak Wood (Quercus petraea)"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6125-2960","authenticated-orcid":false,"given":"Muhammad Tanveer","family":"Munir","sequence":"first","affiliation":[{"name":"Laboratoire Innovation Mat\u00e9riau Bois Habitat Apprentissage (LIMBHA), Ecole Sup\u00e9rieure du Bois, 7 rue Christian Pauc, 44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Pailhories","sequence":"additional","affiliation":[{"name":"Laboratoire HIFIH, UPRES EA3859, SFR 4208, Universit\u00e9 d\u2019Angers, 49933 Angers, France"},{"name":"Laboratoire de Bact\u00e9riologie, CHU Angers, 4 rue Larrey, 49933 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5916-3627","authenticated-orcid":false,"given":"Matthieu","family":"Eveillard","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie, CHU Angers, 4 rue Larrey, 49933 Angers, France"},{"name":"CRCINA and Inserm at Universit\u00e9 de Nantes and Universit\u00e9 d\u2019Angers, 49933 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Irle","sequence":"additional","affiliation":[{"name":"Laboratoire Innovation Mat\u00e9riau Bois Habitat Apprentissage (LIMBHA), Ecole Sup\u00e9rieure du Bois, 7 rue Christian Pauc, 44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Aviat","sequence":"additional","affiliation":[{"name":"Your ResearcH-Bio-Scientific, 307 la Gauterie, 44430 Le Landreau, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1108-6490","authenticated-orcid":false,"given":"Michel","family":"Federighi","sequence":"additional","affiliation":[{"name":"UMR INRA 1014 SECALIM, Oniris, Route de Gachet, CS 40706, CEDEX 03, 44307 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7657-931X","authenticated-orcid":false,"given":"Christophe","family":"Belloncle","sequence":"additional","affiliation":[{"name":"Laboratoire Innovation Mat\u00e9riau Bois Habitat Apprentissage (LIMBHA), Ecole Sup\u00e9rieure du Bois, 7 rue Christian Pauc, 44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.15376\/biores.9.1.273-281","article-title":"Active anti-microbial effects of larch and pine wood on four bacterial strains","volume":"9","author":"Laireiter","year":"2013","journal-title":"BioResources"},{"key":"ref_2","first-page":"7763","article-title":"Antibacterial effects of extracts of Pinus sylvestris and Picea abies against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Streptococcus pneumoniae","volume":"10","author":"Kyyhkynen","year":"2015","journal-title":"BioResources"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s00107-019-01390-2","article-title":"Direct screening method to assess antimicrobial behavior of untreated wood","volume":"77","author":"Munir","year":"2019","journal-title":"Eur. 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