{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T22:33:38Z","timestamp":1780612418395,"version":"3.54.1"},"reference-count":46,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T00:00:00Z","timestamp":1604707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009553","name":"Agentura Pro Zdravotnick\u00fd V\u00fdzkum \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NV18-05-00340"],"award-info":[{"award-number":["NV18-05-00340"]}],"id":[{"id":"10.13039\/501100009553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Enterococci are important bacterial pathogens, and their significance is even greater in the case of vancomycin-resistant enterococci (VRE). The study analyzed the presence of VRE in the gastrointestinal tract (GIT) of hemato-oncological patients. Active screening using selective agars yielded VRE for phenotypic and genotypic analyses. Isolated strains were identified with MALDI-TOF MS, (Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry) their susceptibility to antibiotics was tested, and resistance genes (vanA, vanB, vanC-1, vanC2-C3) and genes encoding virulence factors (asa1, gelE, cylA, esp, hyl) were detected. Pulsed-field gel electrophoresis was used to assess the relationship of the isolated strains. Over a period of three years, 103 VanA-type VRE were identified in 1405 hemato-oncological patients. The most frequently detected virulence factor was extracellular surface protein (84%), followed by hyaluronidase (40%). Unique restriction profiles were observed in 33% of strains; clonality was detected in 67% of isolates. The study found that 7% of hemato-oncological patients carried VRE in their GIT. In all cases, the species identified was Enterococcus faecium. No clone persisted for the entire 3-year study period. However, genetically different clusters were observed for shorter periods of time, no longer than eight months, with identical VRE spreading among patients.<\/jats:p>","DOI":"10.3390\/antibiotics9110785","type":"journal-article","created":{"date-parts":[[2020,11,8]],"date-time":"2020-11-08T19:03:37Z","timestamp":1604862217000},"page":"785","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients"],"prefix":"10.3390","volume":"9","author":[{"given":"Krist\u00fdna","family":"Hricov\u00e1","sequence":"first","affiliation":[{"name":"Department of Microbiology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University Olomouc, 77900 Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u0165\u00e1na","family":"\u0160tosov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University Olomouc, 77900 Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavla","family":"Ku\u010dov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University Olomouc, 77900 Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate\u0159ina","family":"Fi\u0161erov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University Olomouc, 77900 Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Bardo\u0148","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University Olomouc, 77900 Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4621-8868","authenticated-orcid":false,"given":"Milan","family":"Kol\u00e1\u0159","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University Olomouc, 77900 Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,7]]},"reference":[{"key":"ref_1","first-page":"50","article-title":"Vancomycin-resistant enterococci","volume":"24","year":"2018","journal-title":"Klin. 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