{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:44:55Z","timestamp":1787960695911,"version":"build-2784847793"},"reference-count":55,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T00:00:00Z","timestamp":1605830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["No. 2019R1A2B5B03070330, NRF-2017M3A9E4077206"],"award-info":[{"award-number":["No. 2019R1A2B5B03070330, NRF-2017M3A9E4077206"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010418","name":"Institute for Information and Communications Technology Promotion","doi-asserted-by":"publisher","award":["No. 2017-0-01714"],"award-info":[{"award-number":["No. 2017-0-01714"]}],"id":[{"id":"10.13039\/501100010418","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Antibiotic resistance is an important issue affecting humans and livestock. Antimicrobial peptides are promising alternatives to antibiotics. In this study, the antimicrobial peptide Css54, isolated from the venom of C. suffuses, was found to exhibit antimicrobial activity against bacteria such as Listeria monocytogenes, Streptococcus suis, Campylobacter jejuni, and Salmonella typhimurium that cause zoonotic diseases. Moreover, the cytotoxicity and hemolytic activity of Css54 was lower than that of melittin isolated from bee venom. Circular dichroism assays showed that Css54 has an \u03b1-helix structure in an environment mimicking that of bacterial cell membranes. We examined the effect of Css54 on bacterial membranes using N-phenyl-1-naphthylamine, 3,3\u2032-dipropylthiadicarbbocyanine iodides, SYTOX green, and propidium iodide. Our findings suggest that the Css54 peptide kills bacteria by disrupting the bacterial membrane. Moreover, Css54 exhibited antibiofilm activity against L. monocytogenes. Thus, Css54 may be useful as an alternative to antibiotics in humans and animal husbandry.<\/jats:p>","DOI":"10.3390\/antibiotics9110831","type":"journal-article","created":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T02:09:07Z","timestamp":1605838147000},"page":"831","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Scorpion-Venom-Derived Antimicrobial Peptide Css54 Exerts Potent Antimicrobial Activity by Disrupting Bacterial Membrane of Zoonotic Bacteria"],"prefix":"10.3390","volume":"9","author":[{"given":"Jonggwan","family":"Park","sequence":"first","affiliation":[{"name":"Department of Bioinformatics, Kongju National University, Kongju 38065, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Hee","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Chosun University, Gwangju 61452, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Kyoung","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Chosun University, Gwangju 61452, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moon-Chang","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Chosun University, Gwangju 61452, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang Ho","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Kongju National University, Kongju 38065, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoonkyung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Chosun University, Gwangju 61452, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.1093\/bioinformatics\/bty179","article-title":"Deep learning improves antimicrobial peptide recognition","volume":"34","author":"Veltri","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1016\/S1473-3099(16)30388-7","article-title":"China\u2019s national plan to combat antimicrobial resistance","volume":"16","author":"Xiao","year":"2016","journal-title":"Lancet Infect. 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