{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:19:16Z","timestamp":1785795556149,"version":"3.56.0"},"reference-count":26,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T00:00:00Z","timestamp":1607817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016018","name":"Universidad El Bosque","doi-asserted-by":"publisher","award":["PCI-2017-9453"],"award-info":[{"award-number":["PCI-2017-9453"]}],"id":[{"id":"10.13039\/100016018","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: The dissemination of the uropathogenic O25b-ST131 Escherichia coli clone constitutes a threat to public health. We aimed to determine the circulation of E. coli strains belonging to O25b:H4-B2-ST131 and the H30-Rx epidemic subclone causing hospital and community-acquired urinary tract infections (UTI) in Colombia. Methods: Twenty-six nonduplicate, CTX-M group-1-producing isolates causing UTI in the hospital and community were selected for this study. Results: Twenty-two E. coli isolates harboring CTX-M-15, one CTX-M-3, and three CTX-M-55 were identified. Multilocus Sequence Typing (MLST) showed a variety of sequence types (STs), among which, ST131, ST405, and ST648 were reported as epidemic clones. All the E. coli ST131 sequences carried CTX-M-15, from which 80% belonged to the O25b:H4-B2 and H30-Rx pandemic subclones and were associated with virulence factors iss, iha, and sat. E. coli isolates (23\/26) were resistant to ciprofloxacin and associated with amino acid substitutions in quinolone resistance-determining regions (QRDR). We detected two carbapenem-resistant E. coli isolates, one coproducing CTX-M-15, KPC-2, and NDM-1 while the other presented mutations in ompC. Additionally, one isolate harbored the gene mcr-1. Conclusions: Our study revealed the circulation of the E. coli ST131, O25b:H4-B2-H30-Rx subclone, harboring CTX-M-15, QRDR mutations, and other resistant genes. The association of the H30-Rx subclone with sepsis and rapid dissemination warrants attention from the public health and infections control.<\/jats:p>","DOI":"10.3390\/antibiotics9120899","type":"journal-article","created":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T20:56:57Z","timestamp":1607893017000},"page":"899","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Genomic Analysis of CTX-M-Group-1-Producing Extraintestinal Pathogenic E. coli (ExPEc) from Patients with Urinary Tract Infections (UTI) from Colombia"],"prefix":"10.3390","volume":"9","author":[{"given":"Elsa","family":"De La Cadena","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogot\u00e1 110121, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1380-9824","authenticated-orcid":false,"given":"Mar\u00eda Fernanda","family":"Mojica","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogot\u00e1 110121, Colombia"},{"name":"Department of Infectious Diseases, Case Western Reserve University, Cleveland, OH 44106-7164, USA"},{"name":"Research Service, Louis Stokes Veterans Affairs Medical Center, Cleveland, OH 44106-7164, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaly","family":"Castillo","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogot\u00e1 110121, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Correa","sequence":"additional","affiliation":[{"name":"Centro Internacional de Entrenamiento e Investigaciones M\u00e9dicas (CIDEIM), Cali 760031, Colombia"},{"name":"Facultad de Ciencias B\u00e1sicas, Universidad Santiago de Cali, Cali 760031, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6413-5623","authenticated-orcid":false,"given":"Tobias Manuel","family":"Appel","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogot\u00e1 110121, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Garc\u00eda-Betancur","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogot\u00e1 110121, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian Jos\u00e9","family":"Pallares","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogot\u00e1 110121, Colombia"},{"name":"Comit\u00e9 de Infecciones y Vigilancia Epidemiol\u00f3gica, Centro M\u00e9dico Imbanaco, Cali 760031, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1898-9067","authenticated-orcid":false,"given":"Mar\u00eda Virginia","family":"Villegas","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogot\u00e1 110121, Colombia"},{"name":"Comit\u00e9 de Infecciones y Vigilancia Epidemiol\u00f3gica, Centro M\u00e9dico Imbanaco, Cali 760031, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5099","DOI":"10.1128\/AAC.00565-10","article-title":"National Multicenter Study of Predictors and Outcomes of Bacteremia upon Hospital Admission Caused by Enterobacteriaceae Producing Extended-Spectrum \u03b2-Lactamases","volume":"54","author":"Marchaim","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.18.4.657-686.2005","article-title":"Extended-Spectrum \u03b2-Lactamases: A Clinical Update","volume":"18","author":"Paterson","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/S1286-4579(03)00049-2","article-title":"Medical and economic impact of extraintestinal infections due to Escherichia coli: Focus on an increasingly important endemic problem","volume":"5","author":"Russo","year":"2003","journal-title":"Microbes Infect."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1080\/14756366.2017.1344235","article-title":"Beta-lactamase database (BLDB)\u2014Structure and function","volume":"32","author":"Naas","year":"2017","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_5","unstructured":"WHO (2020, February 10). Antimicrobial Resistance: Global Report on Surveillance, Available online: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22247201%5Cnhttp:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?artid=2536104&tool=pmcentrez&rendertype=abstract."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e02162-15","DOI":"10.1128\/mBio.02162-15","article-title":"Evolutionary History of the Global Emergence of the Escherichia coli Epidemic Clone ST131","volume":"7","author":"Stoesser","year":"2016","journal-title":"mBio"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e00377-13","DOI":"10.1128\/mBio.00377-13","article-title":"The Epidemic of Extended-Spectrum-\u03b2-Lactamase-Producing Subclone, H 30-Rx","volume":"4","author":"Price","year":"2013","journal-title":"mBio"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2807\/ese.13.47.19044-en","article-title":"Increasing prevalence of ESBL-producing Enterobacteriaceae in Europe","volume":"13","author":"Coque","year":"2008","journal-title":"Eurosurveillance"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1128\/JCM.00045-11","article-title":"First Characterization of CTX-M-15-Producing Escherichia coli ST131 and ST405 Clones Causing Community-Onset Infections in South America","volume":"49","author":"Ruiz","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1128\/CMR.00125-13","article-title":"Escherichia coli ST131, an Intriguing Clonal Group","volume":"27","author":"Bertrand","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1007\/s10096-015-2379-9","article-title":"Double- and multi-carbapenemase-producers: The excessively armored bacilli of the current decade","volume":"34","author":"Meletis","year":"2015","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1093\/cid\/cir323","article-title":"The Challenges of Antimicrobial Drug Resistance in Greece","volume":"53","author":"Miyakis","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1111\/j.1600-0463.2011.02766.x","article-title":"Antimicrobial susceptibility to parenteral and oral agents in a largely polyclonal collection of CTX-M-14 and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae","volume":"119","author":"Titelman","year":"2011","journal-title":"APMIS"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1128\/AAC.00008-10","article-title":"Genetic Factors Associated with Elevated Carbapenem Resistance in KPC-Producing Klebsiella pneumoniae","volume":"54","author":"Kitchel","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4417","DOI":"10.1128\/JCM.02458-09","article-title":"Evaluation of updated interpretative criteria for categorizing Klebsiella pneumoniae with reduced carbapenem susceptibility","volume":"48","author":"Endimiani","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.drup.2010.08.003","article-title":"Aminoglycoside Modifying Enzymes","volume":"16","author":"Ramirez","year":"2010","journal-title":"Drug Resist. Updates"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12866-019-1493-8","article-title":"Clonal diversity, virulence genes content and subclone status of Escherichia coli sequence type 131: Comparative analysis of E. coli ST131 and non-ST131 isolates from Iran","volume":"19","author":"Hojabri","year":"2019","journal-title":"BMC Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1093\/jac\/dkt519","article-title":"Prevalence of day-care centre children (France) with faecal CTX-M-producing Escherichia coli comprising O25b:H4 and O16:H5 ST131 strains","volume":"69","author":"Blanc","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6385","DOI":"10.1128\/AAC.01604-13","article-title":"Molecular Epidemiology of Escherichia coli Sequence Type 131 and Its H30 and H30-Rx Subclones among Extended-Spectrum-\u03b2-Lactamase-Positive and -Negative E. coli Clinical Isolates from the Chicago Region, 2007 to 2010","volume":"57","author":"Banerjee","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"195","DOI":"10.12688\/f1000research.10609.1","article-title":"Escherichia coli ST131: A multidrug-resistant clone primed for global domination","volume":"6","author":"Pitout","year":"2017","journal-title":"F1000Research"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1128\/AEM.02634-07","article-title":"Evolution of the iss Gene in Escherichia coli","volume":"74","author":"Johnson","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.1128\/IAI.06388-11","article-title":"Virulence of Escherichia coli Clinical Isolates in a Murine Sepsis Model in Relation to Sequence Type ST131 Status, Fluoroquinolone Resistance, and Virulence Genotype","volume":"80","author":"Johnson","year":"2012","journal-title":"Infect. Immun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.1128\/AAC.01707-08","article-title":"Resistance Plasmid Families in Enterobacteriaceae","volume":"53","author":"Carattoli","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1093\/jac\/dkp122","article-title":"Molecular epidemiology of Escherichia coli producing extended-spectrum \u03b2-lactamases in Lugo (Spain): Dissemination of clone O25b:H4-ST131 producing CTX-M-15","volume":"63","author":"Blanco","year":"2009","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1128\/AEM.66.10.4555-4558.2000","article-title":"Rapid and Simple Determination of the Escherichia coli Phylogenetic Group","volume":"66","author":"Clermont","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1111\/j.1365-2958.2006.05172.x","article-title":"Sex and virulence in Escherichia coli: An evolutionary perspective","volume":"60","author":"Wirth","year":"2006","journal-title":"Mol. Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/9\/12\/899\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:44:28Z","timestamp":1760179468000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/9\/12\/899"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,13]]},"references-count":26,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["antibiotics9120899"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics9120899","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,13]]}}}