{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:58:21Z","timestamp":1787313501619,"version":"build-2736575974"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T00:00:00Z","timestamp":1621641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010653","name":"Masarykova Univerzita","doi-asserted-by":"publisher","award":["MUNI\/A\/1425\/2020"],"award-info":[{"award-number":["MUNI\/A\/1425\/2020"]}],"id":[{"id":"10.13039\/501100010653","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Hydrogen sulfide is a toxic compound that can affect various groups of water microorganisms. Photolithotrophic sulfur bacteria including Chromatiaceae and Chlorobiaceae are able to convert inorganic substrate (hydrogen sulfide and carbon dioxide) into organic matter deriving energy from photosynthesis. This process takes place in the absence of molecular oxygen and is referred to as anoxygenic photosynthesis, in which exogenous electron donors are needed. These donors may be reduced sulfur compounds such as hydrogen sulfide. This paper deals with the description of this metabolic process, representatives of the above-mentioned families, and discusses the possibility using anoxygenic phototrophic microorganisms for the detoxification of toxic hydrogen sulfide. Moreover, their general characteristics, morphology, metabolism, and taxonomy are described as well as the conditions for isolation and cultivation of these microorganisms will be presented.<\/jats:p>","DOI":"10.3390\/antiox10060829","type":"journal-article","created":{"date-parts":[[2021,5,23]],"date-time":"2021-05-23T23:58:05Z","timestamp":1621814285000},"page":"829","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Anoxygenic Photosynthesis in Photolithotrophic Sulfur Bacteria and Their Role in Detoxication of Hydrogen Sulfide"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8450-7137","authenticated-orcid":false,"given":"Ivan","family":"Kushkevych","sequence":"first","affiliation":[{"name":"Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5755-4213","authenticated-orcid":false,"given":"Veronika","family":"Bos\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic"},{"name":"Department of Biology, Faculty of Medicine, Masaryk University, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0999-7957","authenticated-orcid":false,"given":"Monika","family":"V\u00edt\u011bzov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9746-3284","authenticated-orcid":false,"given":"Simon K.-M. R.","family":"Rittmann","sequence":"additional","affiliation":[{"name":"Archaea Physiology &amp; Biotechnology Group, Department of Functional and Evolutionary Ecology, Universit\u00e4t Wien, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,22]]},"reference":[{"key":"ref_1","unstructured":"Postgate, J. (1984). The Suphate-Reducing Bacteria, Cambridge University. [2nd ed.]."},{"key":"ref_2","first-page":"237","article-title":"Hydrogen Sulfide: A Toxic Gas Produced by Dissimilatory Sulfate and Sulfur Reduction and Consumed by Microbial Oxidation","volume":"14","author":"Barton","year":"2014","journal-title":"Met. Ions Life Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s00203-019-01625-z","article-title":"Toxicity of Hydrogen Sulfide toward Sulfate-Reducing Bacteria Desulfovibrio Piger Vib-7","volume":"201","author":"Kushkevych","year":"2019","journal-title":"Arch. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Kushkevych, I., Dordevi\u0107, D., Kollar, P., V\u00edt\u011bzov\u00e1, M., and Drago, L. (2019). Hydrogen Sulfide as a Toxic Product in the Small\u2013Large Intestine Axis and Its Role in IBD Development. JCM, 8.","DOI":"10.3390\/jcm8071054"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1007\/s00203-018-1510-6","article-title":"The Diversity of Sulfate-Reducing Bacteria in the Seven Bioreactors","volume":"200","author":"Kushkevych","year":"2018","journal-title":"Arch. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"103","DOI":"10.18388\/abp.2014_845","article-title":"Activity and Kinetic Properties of Phosphotransacetylase from Intestinal Sulfate-Reducing Bacteria","volume":"62","author":"Kushkevych","year":"2015","journal-title":"Acta Biochim. Pol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jare.2020.03.007","article-title":"Possible Synergy Effect of Hydrogen Sulfide and Acetate Produced by Sulfate-Reducing Bacteria on Inflammatory Bowel Disease Development","volume":"27","author":"Kushkevych","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref_8","first-page":"55","article-title":"Hydrogen Sulfide Toxicity in the Gut Environment: Meta-Analysis of Sulfate-Reducing and Lactic Acid Bacteria in Inflammatory Processes","volume":"27","author":"Kushkevych","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Imhoff, J. (2004). Taxonomy and Physiology of Phototrophic Purple Bacteria and Green Sulfur Bacteria. Anoxygenic Photosynthetic Bacteria, Springer.","DOI":"10.1007\/0-387-28021-9_15"},{"key":"ref_10","first-page":"1","article-title":"On the Morphology and Physiology of the Purple and Green Sulfur Bacteria","volume":"3","author":"Niel","year":"1932","journal-title":"Arch. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s002030050615","article-title":"Sulfide Oxidation in the Phototrophic Sulfur Bacterium Chromatium Vinosum","volume":"170","author":"Reinartz","year":"1998","journal-title":"Arch. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Blumenberg, M., Shaaban, M., and Elgaml, A. (2020). Isolation and Purification of Sulfate-Reducing Bacteria. Microorganisms, IntechOpen.","DOI":"10.5772\/intechopen.82960"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/0-387-30747-8_13","article-title":"The Family Chlorobiaceae","volume":"Volume 7","author":"Overmann","year":"2006","journal-title":"The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s00203-002-0432-4","article-title":"Phylogeny of Green Sulfur Bacteria on the Basis of Gene Sequences of 16S RRNA and of the Fenna-Matthews-Olson Protein","volume":"178","author":"Alexander","year":"2002","journal-title":"Arch. Microbiol."},{"key":"ref_15","first-page":"61","article-title":"Chlorophyll Organization and Function in Green Photosynthetic Bacteria*","volume":"67","author":"Olson","year":"2008","journal-title":"Photochem. Photobiol."},{"key":"ref_16","unstructured":"Clayton, R., and Sistrom, W.R. (1978). The Photosynthetic Bacteria, Springer."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0065-2911(08)00002-7","article-title":"Sulfur Metabolism in Phototrophic Sulfur Bacteria","volume":"54","author":"Frigaard","year":"2008","journal-title":"Adv. Microb. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"8135","DOI":"10.1073\/pnas.89.17.8135","article-title":"Photosynthetic Reaction Center Genes in Green Sulfur Bacteria and in Photosystem 1 Are Related","volume":"89","author":"Xie","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Blankenship, R., Olson, J., and Miller, M. (2004). Antenna Complexes from Green Photosynthetic Bacteria. Anoxygenic Photosynthetic Bacteria, Springer.","DOI":"10.1007\/978-94-017-2087-8_6"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/S0005-2728(01)00200-6","article-title":"The Reaction Center of Green Sulfur Bacteria Dedicated to the Memory of Jan Amesz.1","volume":"1507","author":"Hauska","year":"2001","journal-title":"Biochim. Et Biophys. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wang-Otomo, Z.-Y. (2016). Recent Understanding on the Photosystem of Purple Photosynthetic Bacteria. Solar to Chemical Energy Conversion, Springer.","DOI":"10.1007\/978-3-319-25400-5_22"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1021\/jp983722+","article-title":"Photosynthetic Light-Harvesting: Reconciling Dynamics and Structure of Purple Bacterial LH2 Reveals Function of Photosynthetic Unit","volume":"103","author":"Sundstro","year":"1999","journal-title":"J. Phys. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-1-4020-8815-5_6","article-title":"Distribution and Biosynthesis of Carotenoids","volume":"Volume 28","author":"Hunter","year":"2009","journal-title":"The Purple Phototrophic Bacteria"},{"key":"ref_24","unstructured":"Cohen-Bazire, G., Sistrom, W., Vernon, L., and Seeley, G. (1966). The Chlorophylls. Academic Press."},{"key":"ref_25","first-page":"179","article-title":"Biological Sulfide Oxidation: Sulfide-Quinone Reductase (SQR), the Primary Reaction","volume":"4","author":"Griesbeck","year":"2000","journal-title":"Recent Res. Dev. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1093\/molbev\/msg174","article-title":"Single Eubacterial Origin of Eukaryotic Sulfide:Quinone Oxidoreductase, a Mitochondrial Enzyme Conserved from the Early Evolution of Eukaryotes during Anoxic and Sulfidic Times","volume":"20","author":"Theissen","year":"2003","journal-title":"Mol. Biol. Evol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0014-5793(92)80230-E","article-title":"Sulfide Quinone Reductase (SQR) Activity in Chlorobium","volume":"299","author":"Shahak","year":"1992","journal-title":"FEBS Lett."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/s002030050748","article-title":"Chlorobium Ferrooxidans Sp. Nov., a Phototrophic Green Sulfur Bacterium That Oxidizes Ferrous Iron in Coculture with a \u201cGeospirillum\u201d Sp. Strain","volume":"172","author":"Heising","year":"1999","journal-title":"Arch. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"11552","DOI":"10.1021\/bi026032b","article-title":"Mechanism of Sulfide-Quinone Reductase Investigated Using Site-Directed Mutagenesis and Sulfur Analysis","volume":"41","author":"Griesbeck","year":"2002","journal-title":"Biochemistry"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6107","DOI":"10.1128\/JB.183.20.6107-6118.2001","article-title":"Cytochrome Complex Essential for Photosynthetic Oxidation of Both Thiosulfate and Sulfide in Rhodovulum Sulfidophilum","volume":"183","author":"Little","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1007\/BF00248423","article-title":"Search for Polythionates in Cultures of Chromatium Vinosum after Sulfide Incubation","volume":"153","author":"Steudel","year":"1990","journal-title":"Arch. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/BF00291278","article-title":"Polysulfide Utilization by Thiocapsa Roseopersicina","volume":"155","author":"Visscher","year":"1990","journal-title":"Arch. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1007\/0-306-47954-0_39","article-title":"Sulfur Compounds as Photosynthetic Electron Donors","volume":"Volume 2","author":"Brune","year":"2004","journal-title":"Anoxygenic Photosynthetic Bacteria"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Dahl, C. (2008). Inorganic Sulfur Compounds as Electron Donors in Purple Sulfur Bacteria. Sulfur Metabolism in Phototrophic Organisms, Springer.","DOI":"10.1007\/978-1-4020-6863-8_15"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1099\/mic.0.2006\/003954-0","article-title":"Utilization of Solid \u201celemental\u201d Sulfur by the Phototrophic Purple Sulfur Bacterium Allochromatium Vinosum: A Sulfur K-Edge X-ray Absorption Spectroscopy Study","volume":"153","author":"Franz","year":"2007","journal-title":"Microbiology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1111\/j.1574-6976.2000.tb00560.x","article-title":"Microbial Interactions Involving Sulfur Bacteria: Implications for the Ecology and Evolution of Bacterial Communities","volume":"24","author":"Overmann","year":"2000","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/283210a0","article-title":"Evolution and Gene Transfer in Purple Photosynthetic Bacteria","volume":"283","author":"Dickerson","year":"1980","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/BF01732011","article-title":"Procaryote Phylogeny IV: Concerning the Phylogenetic Status of a Photosynthetic Bacterium","volume":"5","author":"Zablen","year":"1975","journal-title":"J. Mol. Evol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1038\/258573a0","article-title":"Chlorophyll Arrangement in a Bacteriochlorophyll Protein from Chlorobium Limicola","volume":"258","author":"Fenna","year":"1975","journal-title":"Nature"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1099\/ijs.0.02403-0","article-title":"Phylogenetic Taxonomy of the Family Chlorobiaceae on the Basis of 16S RRNA and Fmo (Fenna-Matthews-Olson Protein) Gene Sequences","volume":"53","author":"Imhoff","year":"2003","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Olson, J.M., Ormerod, J.G., Amesz, J., Stackebrandt, E., and Tr\u00fcper, H.G. (1988). Growth of Chlorobium Limicola F. Thiosulfatophilum on Polysulfides. Green Photosynthetic Bacteria, Springer.","DOI":"10.1007\/978-1-4613-1021-1"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s11120-009-9510-7","article-title":"Phylogeny and Taxonomy of Chlorobiaceae","volume":"104","author":"Imhoff","year":"2010","journal-title":"Photosynth. Res."},{"key":"ref_43","unstructured":"Pfennig, N., and Tr\u00fcper, H.G. (1974). The Phototrophic Bacteria. Bergey\u2019s Man. Determ. Bacteriol., 24\u201375."},{"key":"ref_44","unstructured":"Struk, M., and Kushkevych, I. (2018). Perspectives of Application of Phototrophic Sulfur Bacteria in Hydrogen Sulfide Utilization. MendelNet, 537\u2013541."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s11157-020-09539-9","article-title":"Biogas Upgrading Methods: Recent Advancements and Emerging Technologies","volume":"19","author":"Struk","year":"2020","journal-title":"Rev. Environ. Sci. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Hatti-Kaul, R., Mamo, G., and Mattiasson, B. (2016). Anaerobes in Biotechnology, Springer.","DOI":"10.1007\/978-3-319-45651-5"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Okoro, O.V., and Sun, Z. (2019). Desulphurisation of Biogas: A Systematic Qualitative and Economic-Based Quantitative Review of Alternative Strategies. Chem. Eng., 3.","DOI":"10.3390\/chemengineering3030076"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/10\/6\/829\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:06:03Z","timestamp":1760162763000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/10\/6\/829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,22]]},"references-count":47,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["antiox10060829"],"URL":"https:\/\/doi.org\/10.3390\/antiox10060829","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,22]]}}}