{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:20:39Z","timestamp":1790630439524,"version":"4.1.0"},"reference-count":27,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P26074, J3983, KLI425, KLI645, W1266, F73"],"award-info":[{"award-number":["P26074, J3983, KLI425, KLI645, W1266, F73"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013699","name":"Bundesministerium f\u00fcr Bildung, Wissenschaft und Forschung","doi-asserted-by":"publisher","award":["Omics Center Graz"],"award-info":[{"award-number":["Omics Center Graz"]}],"id":[{"id":"10.13039\/501100013699","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Austrian Herzfonds","award":["201901"],"award-info":[{"award-number":["201901"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Timely centrifugation of blood for plasma preparation is a key step to ensure high plasma quality for analytics. Delays during preparation can significantly influence readouts of key clinical parameters. However, in a routine clinical environment, a strictly controlled timeline is often not feasible. The next best approach is to control for sample preparation delays by a marker that provides a readout of the time-dependent degradation of the sample. In this study, we explored the usefulness of glutathione status as potential marker of plasma preparation delay. As the concentration of glutathione in erythrocytes is at least two orders of magnitude higher than in plasma, even the slightest leakage of glutathione from the cells can be readily observed. Over the 3 h observation period employed in this study, we observed a linear increase of plasma concentrations of both reduced (GSH) and oxidized glutathione (GSSG). Artificial oxidation of GSH is prevented by rapid alkylation with N-ethylmaleimide directly in the blood sampling vessel as recently published. The observed relative leakage of GSH was significantly higher than that of GSSG. A direct comparison with plasma lactate dehydrogenase activity, a widely employed hemolysis marker, clearly demonstrated the superiority of our approach for quality control. Moreover, we show that the addition of the thiol alkylating reagent NEM directly to the blood tubes does not influence downstream analysis of other clinical parameters. In conclusion, we report that GSH gives an excellent readout of the duration of plasma preparation and the associated pre-analytical errors.<\/jats:p>","DOI":"10.3390\/antiox10060864","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T23:16:46Z","timestamp":1622157406000},"page":"864","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Blood Plasma Quality Control by Plasma Glutathione Status"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7071-2316","authenticated-orcid":false,"given":"Tamara","family":"Tomin","sequence":"first","affiliation":[{"name":"Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Technische Universit\u00e4t Wien, 1060 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9505-6271","authenticated-orcid":false,"given":"Natalie","family":"Bordag","sequence":"additional","affiliation":[{"name":"Center for Biomarker Research in Medicine, CBmed GmbH, 8010 Graz, Austria"},{"name":"Translational Platform, Ludwig Boltzmann Institute for Lung Vascular Research, 8010 Graz, Austria"},{"name":"Department of Dermatology and Venereology, Medical University of Graz, 8036 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7124-2793","authenticated-orcid":false,"given":"Elmar","family":"Z\u00fcgner","sequence":"additional","affiliation":[{"name":"Health Institute for Biomedicine and Health Sciences, Joanneum Research Forschungsgesellschaft mbH, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5944-9724","authenticated-orcid":false,"given":"Abdullah","family":"Al-Baghdadi","sequence":"additional","affiliation":[{"name":"Center for Biomarker Research in Medicine, CBmed GmbH, 8010 Graz, Austria"},{"name":"Division of Endocrinology and Diabetology, Department of Internal Medicine, Medical University of Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1009-7208","authenticated-orcid":false,"given":"Maximilian","family":"Schinagl","sequence":"additional","affiliation":[{"name":"Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Technische Universit\u00e4t Wien, 1060 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3950-0312","authenticated-orcid":false,"given":"Ruth","family":"Birner-Gruenberger","sequence":"additional","affiliation":[{"name":"Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Technische Universit\u00e4t Wien, 1060 Vienna, Austria"},{"name":"Diagnostic and Research Institute of Pathology, Medical University of Graz, 8036 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3249-655X","authenticated-orcid":false,"given":"Matthias","family":"Schittmayer","sequence":"additional","affiliation":[{"name":"Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Technische Universit\u00e4t Wien, 1060 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1021\/pr800545q","article-title":"Standard Operating Procedures for Serum and Plasma Collection: Early Detection Research Network Consensus Statement Standard Operating Procedure Integration Working Group","volume":"8","author":"Tuck","year":"2009","journal-title":"J. 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