{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T18:22:50Z","timestamp":1788805370503,"version":"build-2803163510"},"reference-count":38,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T00:00:00Z","timestamp":1623715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Pisa, MIUR, Italy","award":["\u201cBando per il finanziamento di Dimostratori Tecnologici DT (DT grant)\u201d, acronym \u201cMEDICA\u201d"],"award-info":[{"award-number":["\u201cBando per il finanziamento di Dimostratori Tecnologici DT (DT grant)\u201d, acronym \u201cMEDICA\u201d"]}]},{"name":"CREA Council for Agricultural Research and Economics","award":["\u201cContratto per l\u2019affidamento del servizio di consulenza farmacologica per la caratterizzazione cinetico-chimica del rilascio di acido solfidrico (H2S) da farmaci e molecole di origine naturale in soluzione, in vitro e in vivo e per la valutazione degli ef"],"award-info":[{"award-number":["\u201cContratto per l\u2019affidamento del servizio di consulenza farmacologica per la caratterizzazione cinetico-chimica del rilascio di acido solfidrico (H2S) da farmaci e molecole di origine naturale in soluzione, in vitro e in vivo e per la valutazione degli ef"]}]},{"name":"Italian Ministry of Instruction, University and Research (MIUR)","award":["PON \u201cRicerca e Innovazione\u201d 2014-2020-Azione II, by the COMETA research project \u201cColture autoctone mediterranee e loro valorizzazione con tecnologie avanzate di chimica verde\u201d (Native Mediterranean crops and their enhancement with advanced green chemistry"],"award-info":[{"award-number":["PON \u201cRicerca e Innovazione\u201d 2014-2020-Azione II, by the COMETA research project \u201cColture autoctone mediterranee e loro valorizzazione con tecnologie avanzate di chimica verde\u201d (Native Mediterranean crops and their enhancement with advanced green chemistry"]}]},{"name":"Italian Ministry of University and Research (MIUR)","award":["PRIN 2017XP72RF - Hydrogen Sulfide in the Vascular inflamm-Aging: role, therapeutic Oppor-tunities and development of novel pharmacological tools for age-related cardiovascular diseases (SVAgO)"],"award-info":[{"award-number":["PRIN 2017XP72RF - Hydrogen Sulfide in the Vascular inflamm-Aging: role, therapeutic Oppor-tunities and development of novel pharmacological tools for age-related cardiovascular diseases (SVAgO)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Preservation of vascular wall integrity against degenerative processes associated with ageing, fat-rich diet and metabolic diseases is a timely therapeutical challenge. The loss of endothelial function and integrity leads to cardiovascular diseases and multiorgan inflammation. The protective effects of the H2S-donor erucin, an isothiocyanate purified by Eruca sativa Mill. seeds, were evaluated on human endothelial and vascular smooth muscle cells. In particular, erucin actions were evaluated on cell viability, ROS, caspase 3\/7, inflammatory markers levels and the endothelial hyperpermeability in an inflammatory model associated with high glucose concentrations (25 mM, HG). Erucin significantly prevented the HG-induced decrease in cell viability as well as the increase in ROS, caspase 3\/7 activation, and TNF-\u03b1 and IL-6 levels. Similarly, erucin suppressed COX-2 and NF-\u03baB upregulation associated with HG exposure. Erucin also caused a significant inhibition of p22phox subunit expression in endothelial cells. In addition, erucin significantly prevented the HG-induced increase in endothelial permeability as also confirmed by the quantification of the specific markers VE-Cadherin and ZO-1. In conclusion, our results assess anti-inflammatory and antioxidant effects by erucin in vascular cells undergoing HG-induced inflammation and this protection parallels the preservation of endothelial barrier properties.<\/jats:p>","DOI":"10.3390\/antiox10060961","type":"journal-article","created":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T11:00:33Z","timestamp":1623754833000},"page":"961","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["The H2S-Donor Erucin Exhibits Protective Effects against Vascular Inflammation in Human Endothelial and Smooth Muscle Cells"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2090-7107","authenticated-orcid":false,"given":"Alma","family":"Martelli","sequence":"first","affiliation":[{"name":"Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, Italy"},{"name":"Interdepartmental Research Center \u201cNutrafood: Nutraceutica e Alimentazione per la Salute\u201d, University of Pisa, 56126 Pisa, Italy"},{"name":"Interdepartmental Research Center \u201cBiology and Pathology of Ageing\u201d, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6558-3065","authenticated-orcid":false,"given":"Eugenia","family":"Piragine","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Era","family":"Gorica","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Citi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2431-6248","authenticated-orcid":false,"given":"Lara","family":"Testai","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, Italy"},{"name":"Interdepartmental Research Center \u201cNutrafood: Nutraceutica e Alimentazione per la Salute\u201d, University of Pisa, 56126 Pisa, Italy"},{"name":"Interdepartmental Research Center \u201cBiology and Pathology of Ageing\u201d, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9477-1724","authenticated-orcid":false,"given":"Eleonora","family":"Pagnotta","sequence":"additional","affiliation":[{"name":"Research Centre for Cereal and Industrial Crops, CREA Council for Agricultural Research and Economics, Via di Corticella 128, 40134 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Lazzeri","sequence":"additional","affiliation":[{"name":"Research Centre for Cereal and Industrial Crops, CREA Council for Agricultural Research and Economics, Via di Corticella 128, 40134 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Pecchioni","sequence":"additional","affiliation":[{"name":"Research Centre for Cereal and Industrial Crops, CREA Council for Agricultural Research and Economics, S.S. 673 Km 25,200, 71122 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5374-9694","authenticated-orcid":false,"given":"Valerio","family":"Ciccone","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via A. Moro 2, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosangela","family":"Montanaro","sequence":"additional","affiliation":[{"name":"Department of Science, University of Basilicata, Via dell\u2019Ateneo lucano, 10, 85100 Potenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8374-4432","authenticated-orcid":false,"given":"Lorenzo","family":"Di Cesare Mannelli","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Psychology, Drug Research and Child Health\u2013NEUROFARBA\u2013Section of Pharmacology and Toxicology, University of Florence, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Ghelardini","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Psychology, Drug Research and Child Health\u2013NEUROFARBA\u2013Section of Pharmacology and Toxicology, University of Florence, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0063-659X","authenticated-orcid":false,"given":"Vincenzo","family":"Brancaleone","sequence":"additional","affiliation":[{"name":"Department of Science, University of Basilicata, Via dell\u2019Ateneo lucano, 10, 85100 Potenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8148-7049","authenticated-orcid":false,"given":"Lucia","family":"Morbidelli","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via A. Moro 2, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Calderone","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, Italy"},{"name":"Interdepartmental Research Center \u201cNutrafood: Nutraceutica e Alimentazione per la Salute\u201d, University of Pisa, 56126 Pisa, Italy"},{"name":"Interdepartmental Research Center \u201cBiology and Pathology of Ageing\u201d, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1111\/bph.14039","article-title":"Targeting inflammation to reduce cardiovascular disease risk","volume":"174","author":"Maffia","year":"2017","journal-title":"Br. J. 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