{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:35:14Z","timestamp":1791945314058,"version":"4.3.4"},"reference-count":30,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T00:00:00Z","timestamp":1628812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Grant Agency of the Slovak Republic","award":["VEGA 1\/0064\/21, 2\/0079\/19, 2\/0091\/21, 2\/0053\/19"],"award-info":[{"award-number":["VEGA 1\/0064\/21, 2\/0079\/19, 2\/0091\/21, 2\/0053\/19"]}]},{"DOI":"10.13039\/501100005357","name":"Slovak Research and Development Agency","doi-asserted-by":"publisher","award":["APVV-19-0154, APVV-17-0384"],"award-info":[{"award-number":["APVV-19-0154, APVV-17-0384"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Superoxide radical anion (O2\u2022\u2212) and its derivatives regulate numerous physiological and pathological processes, which are extensively studied. The aim of our work was to utilize KO2 as a source of O2\u2022\u2212 and the electron paramagnetic resonance (EPR) spin trapping 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide (BMPO) technique for the preparation of \u2022BMPO-OOH and\/or \u2022BMPO-OH radicals in water solution without DMSO. The method distinguishes the interactions of various compounds with \u2022BMPO-OOH and\/or \u2022BMPO-OH radicals over time. Here, we show that the addition of a buffered BMPO-HCl mixture to powdered KO2 formed relatively stable \u2022BMPO-OOH and \u2022BMPO-OH radicals and H2O2, where the \u2022BMPO-OOH\/OH ratio depended on the pH. At a final pH of ~6.5\u20138.0, the concentration of \u2022BMPO-OOH radicals was \u226520 times higher than that of \u2022BMPO-OH, whereas at pH 9.0\u201310.0, the \u2022BMPO-OH radicals prevailed. The \u2022BMPO-OOH\/OH radicals effectively cleaved the plasmid DNA. H2S decreased the concentration of \u2022BMPO-OOH\/OH radicals, whereas the selenium derivatives 1-methyl-4-(3-(phenylselanyl) propyl) piperazine and 1-methyl-4-(4-(phenylselanyl) butyl) piperazine increased the proportion of \u2022BMPO-OH over the \u2022BMPO-OOH radicals. In conclusion, the presented approach of using KO2 as a source of O2\u2022\u2212\/H2O2 and EPR spin trap BMPO for the preparation of \u2022BMPO-OOH\/OH radicals in a physiological solution could be useful to study the biological effects of radicals and their interactions with compounds.<\/jats:p>","DOI":"10.3390\/antiox10081286","type":"journal-article","created":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T09:22:38Z","timestamp":1628846558000},"page":"1286","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["EPR Study of KO2 as a Source of Superoxide and \u2022BMPO-OH\/OOH Radical That Cleaves Plasmid DNA and Detects Radical Interaction with H2S and Se-Derivatives"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6331-1004","authenticated-orcid":false,"given":"Anton","family":"Misak","sequence":"first","affiliation":[{"name":"Biomedical Research Center, Department of Molecular Physiology, Institute of Clinical and Translational Research, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vlasta","family":"Brezova","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry and Chemical Physics, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinsk\u00e9ho 9, 81237 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9120-5084","authenticated-orcid":false,"given":"Miroslav","family":"Chovanec","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Department of Genetics, Cancer Research Institute, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karol","family":"Luspai","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry and Chemical Physics, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinsk\u00e9ho 9, 81237 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2911-2607","authenticated-orcid":false,"given":"Muhammad Jawad","family":"Nasim","sequence":"additional","affiliation":[{"name":"Division of Bioorganic Chemistry, School of Pharmacy, University of Saarland, D-66123 Saarbruecken, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2846-7924","authenticated-orcid":false,"given":"Marian","family":"Grman","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Department of Molecular Physiology, Institute of Clinical and Translational Research, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1943-0858","authenticated-orcid":false,"given":"Lenka","family":"Tomasova","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Department of Molecular Physiology, Institute of Clinical and Translational Research, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6288-7220","authenticated-orcid":false,"given":"Claus","family":"Jacob","sequence":"additional","affiliation":[{"name":"Division of Bioorganic Chemistry, School of Pharmacy, University of Saarland, D-66123 Saarbruecken, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8329-3563","authenticated-orcid":false,"given":"Karol","family":"Ondrias","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Department of Molecular Physiology, Institute of Clinical and Translational Research, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.freeradbiomed.2018.02.033","article-title":"Superoxide radicals react with peptide-derived tryptophan radicals with very high rate constants to give hydroperoxides as major products","volume":"118","author":"Carroll","year":"2018","journal-title":"Free Radic. 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