{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:56:16Z","timestamp":1788472576368,"version":"build-2803163510"},"reference-count":51,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T00:00:00Z","timestamp":1632528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sociedad Espa\u00f1ola de Neonatologia","award":["Permio Carmen Pedraz"],"award-info":[{"award-number":["Permio Carmen Pedraz"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Hypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidative damage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Our aim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day rat pups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated, hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia + allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH\/GSSG ratio and carbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma and cerebrospinal fluid, and 8-iso-prostaglandin F2\u03b1 was measured in brain tissue. Plasma 2,2\u2032\u2013azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that received allopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferric reducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantly reduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotection in HIE.<\/jats:p>","DOI":"10.3390\/antiox10101523","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T01:59:47Z","timestamp":1632707987000},"page":"1523","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy"],"prefix":"10.3390","volume":"10","author":[{"given":"Cristina","family":"Dur\u00e1n Fern\u00e1ndez-Feij\u00f3o","sequence":"first","affiliation":[{"name":"Department of Neonatology, Hospital \u00c1lvaro Cunqueiro, EOXI, 36312 Vigo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0128-4215","authenticated-orcid":false,"given":"Javier","family":"Rodr\u00edguez-Fanjul","sequence":"additional","affiliation":[{"name":"Neonatal Intensive Care Unit, Paediatrics Department, Hospital Germans Trias i Pujol, Universitat Autonoma de Barcelona, 08916 Badalona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Lopez-Abat","sequence":"additional","affiliation":[{"name":"Department of Neonatology, BCNatal|Barcelona Center for Maternal Fetal and Neonatal Medicine Hospital Sant Joan de D\u00e9u and Hospital Cl\u00ednic, University of Barcelona, 08950 Esplugues de Llobregat, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Hadley","sequence":"additional","affiliation":[{"name":"Vanderbilt University School of Medicine, Nashville, TN 37232, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Cavia-Saiz","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Science, Facutlty of Sciences, University of Burgos, 09001 Burgos, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Mu\u00f1iz","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Science, Facutlty of Sciences, University of Burgos, 09001 Burgos, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8883-5181","authenticated-orcid":false,"given":"Juan","family":"Arnaez","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Hospital Universitario de Burgos, NeNe Foundation, 09006 Burgos, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Ram\u00f3n","family":"Fern\u00e1ndez-Lorenzo","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Hospital \u00c1lvaro Cunqueiro, EOXI, 36312 Vigo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Camprub\u00ed Camprub\u00ed","sequence":"additional","affiliation":[{"name":"Department of Neonatology, BCNatal|Barcelona Center for Maternal Fetal and Neonatal Medicine Hospital Sant Joan de D\u00e9u and Hospital Cl\u00ednic, University of Barcelona, 08950 Esplugues de Llobregat, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1038\/pr.2013.203","article-title":"Estimates of neonatal morbidities and disabilities at regional and global levels for 2010: Introduction, methods overview, and relevant findings from the global burden of disease study","volume":"74","author":"Blencowe","year":"2013","journal-title":"Pediatr. 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