{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T22:11:28Z","timestamp":1769551888928,"version":"3.49.0"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T00:00:00Z","timestamp":1644796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The rhizome of Anemarrhena asphodeloides Bunge (AA, family Liliaceae) is a famous and frequently used herbal drug in the traditional medicine of Northeast Asia, under vernacular name \u201czhimu\u201d. A. asphodeloides has been used as an anti-inflammatory, antipyretic, anti-platelet aggregation, anti-depressant, and anti-diabetic agent in traditional Chinese medicine. We examined the antioxidant, anti-acetylcholinesterase (AChE), and anti-\u03b1-glucosidase activities of various solvent extracts and the main bioactive compounds from the rhizome of A. asphodeloides. Acetone extract exhibited comparatively high antioxidant activities by 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 2,2\u2032-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and ferric-reducing antioxidant power (FRAP) assays. A water extract exhibited relatively strong antioxidant activity by superoxide radical scavenging test. Furthermore, dichloromethane, chloroform, and n-hexane extracts showed significant anti-\u03b1-glucosidase activities. Finally, ethanol and dichloromethane extracts exhibited relatively strong AChE inhibitory activity. HPLC analysis was used to examine and compare various solvent extracts for their compositions of isolates. We isolated four major chemical constituents and analyzed their antioxidant, anti-\u03b1-glucosidase, and AChE inhibitory activities. The bioactivity assays showed that mangiferin displayed the most potential antioxidant activities via FRAP, ABTS, DPPH, and superoxide assays and also exhibited the most effective anti-AChE and anti-\u03b1-glucosidase activities among all the isolates. The present study suggests that A. asphodeloides and its active extracts and components are worth further investigation and might be expected to develop as a candidate for the treatment or prevention of oxidative stress-related diseases, AChE inhibition, and hyperglycemia.<\/jats:p>","DOI":"10.3390\/antiox11020385","type":"journal-article","created":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T20:26:42Z","timestamp":1644870402000},"page":"385","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Comparison of Various Solvent Extracts and Major Bioactive Components from Unsalt-Fried and Salt-Fried Rhizomes of Anemarrhena asphodeloides for Antioxidant, Anti-\u03b1-Glucosidase, and Anti-Acetylcholinesterase Activities"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3063-2442","authenticated-orcid":false,"given":"Yi-Cheng","family":"Chu","sequence":"first","affiliation":[{"name":"Institute of Traditional Medicine, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Syun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Jen","family":"Cheng","sequence":"additional","affiliation":[{"name":"Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Ling","family":"Fu","sequence":"additional","affiliation":[{"name":"Institute of Traditional Medicine, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5680-9533","authenticated-orcid":false,"given":"Jih-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Traditional Medicine, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan"},{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan"},{"name":"Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e13472","DOI":"10.1111\/jfbc.13472","article-title":"In vitro antioxidant, anticholinesterases, anti-\u03b1-amylase, and anti-\u03b1-glucosidase effects of Algerian Salvia aegyptiaca and Salvia verbenaca","volume":"44","author":"Mamache","year":"2020","journal-title":"J. Food Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.biopha.2015.12.024","article-title":"The influence of common free radicals and antioxidants on development of Alzheimer\u2019s disease","volume":"78","author":"Oniszczuk","year":"2016","journal-title":"Biomed. Pharmacother."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1113\/jphysiol.2007.135871","article-title":"Diabetes associated cell stress and dysfunction: Role of mitochondrial and non-mitochondrial ros production and activity","volume":"583","author":"Newsholme","year":"2007","journal-title":"J. Physiol. Lond."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1515\/znc-2007-11-1210","article-title":"Screening of various phenolic acids and flavonoid derivatives for their anticholinesterase potential","volume":"62","author":"Orhan","year":"2007","journal-title":"Z. Naturforsch. C"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.indcrop.2015.05.034","article-title":"LC\u2013MS\/MS analysis, antioxidant and anticholinergic properties of galanga (Alpinia officinarum Hance) rhizomes","volume":"74","author":"Namiesnik","year":"2015","journal-title":"Ind. Crop. Prod."},{"key":"ref_6","first-page":"728983","article-title":"New acetylcholinesterase inhibitors for Alzheimer\u2019s disease","volume":"2012","author":"Mehta","year":"2012","journal-title":"Int. J. Alzheimer\u2019s Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"10","DOI":"10.5742\/MEWFM.2018.93378","article-title":"Acarbose versus metformin in the treatment of metabolic syndrome","volume":"16","author":"Helvaci","year":"2018","journal-title":"World Fam. Med."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chu, Y.-C., Chang, C.-H., Liao, H.-R., Fu, S.-L., and Chen, J.-J. (2021). Anti-cancer and anti-inflammatory activities of three new chromone derivatives from the marine-derived Penicillium citrinum. Mar. Drugs, 19.","DOI":"10.3390\/md19080408"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1021\/jf980707t","article-title":"Detection of antifungal activity in Anemarrhena asphodeloides by sensitive BCT method and isolation of its active compound","volume":"47","author":"Iida","year":"1999","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1021\/np50093a006","article-title":"Isolation of pseudoprototimosaponin AIII from rhizomes of Anemarrhena asphodeloides and its hypoglycemic activity in streptozotocin-induced diabetic mice","volume":"56","author":"Nakashima","year":"1993","journal-title":"J. Nat. Prod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/S0223-5234(00)00151-3","article-title":"Spirostanols obtained by cyclization of pseudosaponin derivatives and comparison of anti-platelet agglutination activities of spirostanol glycosides","volume":"35","author":"Tobari","year":"2000","journal-title":"Eur. J. Med. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1021\/np1007735","article-title":"Cytotoxic and antineoplastic activity of timosaponin AIII for human cancer cells","volume":"74","author":"Kang","year":"2011","journal-title":"J. Nat. Prod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"357","DOI":"10.3109\/08923970903383889","article-title":"Inhibitory effects of steroidal timosaponins isolated from the rhizomes of Anemarrhena asphodeloides against passive cutaneous anaphylaxis reaction and pruritus","volume":"32","author":"Lee","year":"2010","journal-title":"Immunopharmacol. Immunotoxicol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.pbb.2009.04.021","article-title":"Timosaponin AIII, a saponin isolated from Anemarrhena asphodeloides, ameliorates learning and memory deficits in mice","volume":"93","author":"Lee","year":"2009","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1002\/bmc.3575","article-title":"Determination of the chemical constituents of the different processed products of Anemarrhena asphodeloides rhizomes by high-performance liquid chromatography quadrupole time-of-flight mass spectrometry","volume":"30","author":"Peng","year":"2016","journal-title":"Biomed. Chromatogr."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Li, C.-W., Chu, Y.-C., Huang, C.-Y., Fu, S.-L., and Chen, J.-J. (2020). Evaluation of antioxidant and anti-\u03b1-glucosidase activities of various solvent extracts and major bioactive components from the seeds of Myristica fragrans. Molecules, 25.","DOI":"10.3390\/molecules25215198"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.jfda.2013.11.001","article-title":"Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica","volume":"22","author":"Do","year":"2014","journal-title":"J. Food Drug Anal."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1271\/bbb.58.1780","article-title":"A Simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish","volume":"58","author":"Takao","year":"1994","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved abts radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.foodchem.2008.11.053","article-title":"Antioxidant activity of rhizoma Smilacis Glabrae Extracts and its key constituent-astilbin","volume":"115","author":"Zhang","year":"2009","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.jfca.2006.01.003","article-title":"Comparison of ABTS, DPPH, FRAP, and ORAC Assays for estimating antioxidant activity from guava fruit extracts","volume":"19","author":"Thaipong","year":"2006","journal-title":"J. Food Compos. Anal."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/j.foodchem.2009.07.032","article-title":"Polyphenol composition and in vitro antioxidant activity of amaranth, quinoa buckwheat and wheat as affected by sprouting and baking","volume":"119","author":"Wijngaard","year":"2010","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1002\/bmc.2761","article-title":"Rapid screening and identification of \u03b1-glucosidase inhibitors from mulberry leaves using enzyme-immobilized magnetic beads coupled with HPLC\/MS and NMR","volume":"27","author":"Tao","year":"2013","journal-title":"Biomed. Chromatogr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6663","DOI":"10.3390\/molecules18066663","article-title":"Acetylcholinesterase inhibition and in vitro and in vivo antioxidant activities of Ganoderma lucidum grown on germinated brown rice","volume":"18","author":"Hasnat","year":"2013","journal-title":"Molecules"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J. Comput. Chem."},{"key":"ref_26","unstructured":"BIOVIA (2021). Discovery Studio Client 2021, Dassault Syst\u00e8mes. v.21.1.0."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4205","DOI":"10.1111\/j.1742-4658.2010.07810.x","article-title":"Crystal structures of isomaltase from Saccharomyces cerevisiae and in complex with its competitive inhibitor maltose","volume":"277","author":"Yamamoto","year":"2010","journal-title":"FEBS J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1016\/j.jchromb.2011.05.013","article-title":"Isolation and identification of antiplasmodial N-alkylamides from Spilanthes acmella flowers using centrifugal partition chromatography and ESI-ITTOF-MS","volume":"879","author":"Mbeunkui","year":"2011","journal-title":"J. Chromatogr. B"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/s11130-009-0127-y","article-title":"Antioxidant activities of four edible seaweeds from the southern coast of Thailand","volume":"64","author":"Yangthong","year":"2009","journal-title":"Plant Food Hum. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.foodchem.2003.12.008","article-title":"Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb) buds and lavender (Lavandula stoechas L.)","volume":"87","author":"Beydemir","year":"2004","journal-title":"Food Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3122","DOI":"10.1021\/jf0116606","article-title":"Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: A comparative study","volume":"50","author":"Ou","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.lwt.2012.09.032","article-title":"Antioxidant activity of the mangiferin inclusion complex with \u03b2-cyclodextrin","volume":"51","author":"Ferreira","year":"2013","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"13464","DOI":"10.1021\/jp4067235","article-title":"Binding mechanism and synergetic effects of xanthone derivatives as noncompetitive \u03b1-glucosidase inhibitors: A theoretical and experimental study","volume":"117","author":"Liu","year":"2013","journal-title":"J. Phys. Chem. B"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"113616","DOI":"10.1016\/j.jpba.2020.113616","article-title":"HPLC fingerprinting-based multivariate analysis of phenolic compounds in mango leaves varieties: Correlation to their antioxidant activity and in silico \u03b1-glucosidase inhibitory ability","volume":"191","author":"Wu","year":"2020","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.sajb.2018.02.001","article-title":"Mangiferin from Mangifera indica fruits reduces post-prandial glucose level by inhibiting \u03b1-glucosidase and \u03b1-amylase activity","volume":"120","author":"Sekar","year":"2019","journal-title":"S. Afr. J. Bot."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.1517\/13543784.9.10.2393","article-title":"Review of the acetylcholinesterase inhibitor galanthamine","volume":"9","author":"Sramek","year":"2000","journal-title":"Expert Opin. Investig. Drugs"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.ejphar.2012.02.042","article-title":"Neuropharmacological effect of mangiferin on brain cholinesterase and brain biogenic amines in the management of Alzheimer\u2019s disease","volume":"683","author":"Biradar","year":"2012","journal-title":"Eur. J. Pharmacol."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/11\/2\/385\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:19:30Z","timestamp":1760134770000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/11\/2\/385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,14]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["antiox11020385"],"URL":"https:\/\/doi.org\/10.3390\/antiox11020385","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,14]]}}}