{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:32:50Z","timestamp":1788172370854,"version":"build-2803163510"},"reference-count":34,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,3,26]],"date-time":"2022-03-26T00:00:00Z","timestamp":1648252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Septic shock is a major cause of mortality in ICU patients, its pathophysiology is complex and not properly understood. Oxidative stress seems to be one of the most important mechanisms of shock progression to multiple organ failure. In the present pilot study, we have analysed eight oxidative-stress-related biomarkers in seven consecutive time points (i.e., the first seven days) in 21 septic shock patients admitted to the ICU. Our objective was to describe the kinetics of four biomarkers related to pro-oxidative processes (nitrite\/nitrate, malondialdehyde, 8-oxo-2\u2032-deoxyguanosine, soluble endoglin) compared to four biomarkers of antioxidant processes (the ferric reducing ability of plasma, superoxide dismutase, asymmetric dimethylarginine, mid-regional pro-adrenomedullin) and four inflammatory biomarkers (CRP, IL-6, IL-10 and neopterin). Furthermore, we analysed each biomarker\u2019s ability to predict mortality at the time of admission and 12 h after admission. Although a small number of study subjects were recruited, we have identified four promising molecules for further investigation: soluble endoglin, superoxide dismutase, asymmetric dimethylarginine and neopterin.<\/jats:p>","DOI":"10.3390\/antiox11040640","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T21:27:51Z","timestamp":1648416471000},"page":"640","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Kinetics of Biomarkers of Oxidative Stress in Septic Shock: A Pilot Study"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9021-4947","authenticated-orcid":false,"given":"Martin","family":"Helan","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology and Intensive Care, St. Anne\u2019s University Hospital Brno, 656 91 Brno, Czech Republic"},{"name":"International Clinical Research Center (ICRC), St. Anne\u2019s University Hospital Brno, 656 91 Brno, Czech Republic"},{"name":"Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6911-2575","authenticated-orcid":false,"given":"Jan","family":"Malaska","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"},{"name":"Department of Anaesthesiology and Intensive Care Medicine, University Hospital Brno, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Tomandl","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Jarkovsky","sequence":"additional","affiliation":[{"name":"Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Helanova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"},{"name":"Department of Internal Medicine and Cardiology, University Hospital Brno, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klara","family":"Benesova","sequence":"additional","affiliation":[{"name":"Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Sitina","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology and Intensive Care, St. Anne\u2019s University Hospital Brno, 656 91 Brno, Czech Republic"},{"name":"International Clinical Research Center (ICRC), St. Anne\u2019s University Hospital Brno, 656 91 Brno, Czech Republic"},{"name":"Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milan","family":"Dastych","sequence":"additional","affiliation":[{"name":"Department of Laboratory Methods, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Ondrus","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"},{"name":"Department of Internal Medicine and Cardiology, University Hospital Brno, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Pavkova Goldbergova","sequence":"additional","affiliation":[{"name":"Department of Pathological Physiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Gal","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"},{"name":"Department of Anaesthesiology and Intensive Care Medicine, University Hospital Brno, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Lokaj","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"},{"name":"Department of Internal Medicine and Cardiology, University Hospital Brno, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2944-9605","authenticated-orcid":false,"given":"Marie","family":"Tomandlova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Parenica","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic"},{"name":"Department of Internal Medicine and Cardiology, University Hospital Brno, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1001\/jama.2016.0287","article-title":"The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3)","volume":"315","author":"Singer","year":"2016","journal-title":"JAMA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5985209","DOI":"10.1155\/2017\/5985209","article-title":"Role of Oxidative Stress and Mitochondrial Dysfunction in Sepsis and Potential Therapies","volume":"2017","author":"Mantzarlis","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.burns.2016.09.023","article-title":"Oxidative Stress in Sepsis: Pathophysiological Implications Justifying Antioxidant Co-Therapy","volume":"43","author":"Prauchner","year":"2017","journal-title":"Burns"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1159\/000503718","article-title":"Activation of the Nitric Oxide Pathway and Acute Myocardial Infarction Complicated by Acute Kidney Injury","volume":"10","author":"Parenica","year":"2020","journal-title":"Cardiorenal Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.freeradbiomed.2021.07.040","article-title":"Prognostic Value of Oxidative Stress in Patients with Acute Myocardial Infarction Complicated by Cardiogenic Shock: A Prospective Cohort Study","volume":"174","author":"Tomandlova","year":"2021","journal-title":"Free Radic. Biol. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1089\/ars.2015.6317","article-title":"Clinical Relevance of Biomarkers of Oxidative Stress","volume":"23","author":"Frijhoff","year":"2015","journal-title":"Antioxid. Redox Signal."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ayala, J.C., Grismaldo, A., Sequeda-Casta\u00f1eda, L.G., Aristiz\u00e1bal-Pach\u00f3n, A.F., and Morales, L. (2021). Oxidative Stress in ICU Patients: ROS as Mortality Long-Term Predictor. Antioxidants, 10.","DOI":"10.3390\/antiox10121912"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"World Medical Association (2013). World Medical Association Declaration of Helsinki: Ethical Principles for Medical Research Involving Human Subjects. JAMA, 310, 2191\u20132194.","DOI":"10.1001\/jama.2013.281053"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/BF02490453","article-title":"Evaluation of a Sensitive HPLC Method for the Determination of Malondialdehyde, and Application of the Method to Different Biological Materials","volume":"52","author":"Khoschsorur","year":"2000","journal-title":"Chromatographia"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1111\/j.1365-2362.2010.02465.x","article-title":"8-Hydroxy-2\u2019-Deoxyguanosine Is a Prognostic Mediator for Cardiac Event","volume":"41","author":"Suzuki","year":"2011","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The Ferric Reducing Ability of Plasma (FRAP) as a Measure of \u201cAntioxidant Power\u201d: The FRAP Assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Gieseg, S.P., Baxter-Parker, G., and Lindsay, A. (2018). Neopterin, Inflammation, and Oxidative Stress: What Could We Be Missing?. Antioxidants, 7.","DOI":"10.3390\/antiox7070080"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"175","DOI":"10.2174\/1389200024605082","article-title":"Neopterin as a Marker for Immune System Activation","volume":"3","author":"Murr","year":"2002","journal-title":"Curr. Drug Metab."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1016\/S0140-6736(00)02063-8","article-title":"Compartmentalised Inducible Nitric-Oxide Synthase Activity in Septic Shock","volume":"355","author":"Annane","year":"2000","journal-title":"Lancet"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.niox.2014.09.004","article-title":"Endothelial (NOS3 E298D) and Inducible (NOS2 Exon 22) Nitric Oxide Synthase Polymorphisms, as Well as Plasma NOx, Influence Sepsis Development","volume":"42","author":"Martin","year":"2014","journal-title":"Nitric Oxide Biol. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/j.1365-2265.2010.03817.x","article-title":"Characteristics of Plasma NOx Levels in Severe Sepsis: High Interindividual Variability and Correlation with Illness Severity, but Lack of Correlation with Cortisol Levels","volume":"73","author":"Ho","year":"2010","journal-title":"Clin. Endocrinol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1186\/cc12538","article-title":"Differentiated control of deranged nitric oxide metabolism: A therapeutic option in sepsis?","volume":"17","author":"Lupp","year":"2013","journal-title":"Crit. Care."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/joim.12331","article-title":"Endothelial Barrier Dysfunction in Septic Shock","volume":"277","author":"Opal","year":"2015","journal-title":"J. Intern. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1007\/s00592-014-0670-3","article-title":"Oscillating Glucose and Constant High Glucose Induce Endoglin Expression in Endothelial Cells: The Role of Oxidative Stress","volume":"52","author":"Pujadas","year":"2015","journal-title":"Acta Diabetol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1159\/000448996","article-title":"High Levels of Soluble Endoglin Induce a Proinflammatory and Oxidative-Stress Phenotype Associated with Preserved NO-Dependent Vasodilatation in Aortas from Mice Fed a High-Fat Diet","volume":"53","author":"Jezkova","year":"2016","journal-title":"J. Vasc. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"34","DOI":"10.3389\/fgene.2015.00034","article-title":"Contribution of Oxidative Stress to Endothelial Dysfunction in Hereditary Hemorrhagic Telangiectasia","volume":"6","author":"Jerkic","year":"2015","journal-title":"Front. Genet."},{"key":"ref_22","first-page":"2723","article-title":"Expression of Endoglin Isoforms in the Myeloid Lineage and Their Role during Aging and Macrophage Polarization","volume":"127","author":"Aristorena","year":"2014","journal-title":"J. Cell Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3970","DOI":"10.1038\/onc.2013.386","article-title":"Antibody-Directed Coupling of Endoglin and MMP-14 Is a Key Mechanism for Endoglin Shedding and Deregulation of TGF-\u03b2 Signaling","volume":"33","author":"Kumar","year":"2014","journal-title":"Oncogene"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ojeda-Fern\u00e1ndez, L., Recio-Poveda, L., Aristorena, M., Lastres, P., Blanco, F.J., Sanz-Rodr\u00edguez, F., Gallardo-Vara, E., de las Casas-Engel, M., Corb\u00ed, \u00c1., and Arthur, H.M. (2016). Mice Lacking Endoglin in Macrophages Show an Impaired Immune Response. PLoS Genet., 12.","DOI":"10.1371\/journal.pgen.1005935"},{"key":"ref_25","first-page":"403","article-title":"Atorvastatin-Induced Endothelial Nitric Oxide Synthase Expression in Endothelial Cells Is Mediated by Endoglin","volume":"66","author":"Zemankova","year":"2015","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Carr, A.C., Bozonet, S., Pullar, J., Spencer, E., Rosengrave, P., and Shaw, G. (2021). Neutrophils Isolated from Septic Patients Exhibit Elevated Uptake of Vitamin C and Normal Intracellular Concentrations despite a Low Vitamin C Milieu. Antioxidants, 10.","DOI":"10.3390\/antiox10101607"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.febslet.2009.11.063","article-title":"Adrenomedullin Protects against Hypoxia\/Reoxygenation-Induced Cell Death by Suppression of Reactive Oxygen Species via Thiol Redox Systems","volume":"584","author":"Kim","year":"2010","journal-title":"FEBS Lett."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3331","DOI":"10.1210\/en.2003-1583","article-title":"Antioxidant Effect of Adrenomedullin on Angiotensin II-Induced Reactive Oxygen Species Generation in Vascular Smooth Muscle Cells","volume":"145","author":"Yoshimoto","year":"2004","journal-title":"Endocrinology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1097\/00003246-199811000-00016","article-title":"Use of the SOFA Score to Assess the Incidence of Organ Dysfunction\/Failure in Intensive Care Units: Results of a Multicenter, Prospective Study. Working Group on \u201cSepsis-Related Problems\u201d of the European Society of Intensive Care Medicine","volume":"26","author":"Vincent","year":"1998","journal-title":"Crit. Care Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1513\/AnnalsATS.201608-656BC","article-title":"Thiamine as a Renal Protective Agent in Septic Shock. A Secondary Analysis of a Randomized, Double-Blind, Placebo-Controlled Trial","volume":"14","author":"Moskowitz","year":"2017","journal-title":"Ann. Am. Thorac. Soc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"94","DOI":"10.4103\/2279-042X.179569","article-title":"Effect of High-Dose Ascorbic Acid on Vasopressor\u2019s Requirement in Septic Shock","volume":"5","author":"Zabet","year":"2016","journal-title":"J. Res. Pharm. Pract."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1177\/0885066617696850","article-title":"N-Acetylcysteine\u2019s Role in Sepsis and Potential Benefit in Patients with Microcirculatory Derangements","volume":"33","author":"Chertoff","year":"2018","journal-title":"J. Intensive Care Med."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Fang, M., Zou, T., Yang, X., Zhang, Z., Cao, P., Han, J., Duan, Y., Ruan, B.-F., and Li, Q.-S. (2021). Discovery of Novel Pterostilbene Derivatives That Might Treat Sepsis by Attenuating Oxidative Stress and Inflammation through Modulation of MAPKs\/NF-\u039aB Signaling Pathways. Antioxidants, 10.","DOI":"10.3390\/antiox10091333"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/j.chest.2016.11.036","article-title":"Hydrocortisone, Vitamin C, and Thiamine for the Treatment of Severe Sepsis and Septic Shock: A Retrospective Before-After Study","volume":"151","author":"Marik","year":"2017","journal-title":"Chest"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/11\/4\/640\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:44:04Z","timestamp":1760136244000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/11\/4\/640"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,26]]},"references-count":34,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["antiox11040640"],"URL":"https:\/\/doi.org\/10.3390\/antiox11040640","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,26]]}}}