{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:09:37Z","timestamp":1782864577915,"version":"3.54.5"},"reference-count":38,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Theodor and Ida Herzog Egli foundation","award":["No specific number"],"award-info":[{"award-number":["No specific number"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Ischemia-reperfusion injury compromises short- and long-term outcomes after lung transplantation. The scarce existing data on NAD+ suggest effects on hypoxia-induced vasoconstriction, on reactive oxygen species and on tampering inflammation. We exposed rat lungs to 14 h of cold ischemic storage and perfused them in a rat ex vivo lung perfusion (EVLP) system for 4 h. A control group (n = 6) was compared to groups receiving 100 \u00b5M (n = 6) or 200 \u00b5M NAD+ (n = 6) in the preservation solution and groups receiving 200 \u00b5M (n = 4) or 2000 \u00b5M (n = 6) NAD+ every 30 min in the perfusate, starting at 1 h of EVLP. Compared to the control, significant effects were only achieved in the 2000 \u00b5M NAD+ group. During the 4 h of EVLP, we monitored higher vascular flow, lower mean pulmonary arterial pressure and increased oxygenation capacity. Tissue inflammation estimated with the myeloperoxidase assay was lower in the 2000 \u00b5M NAD+ group. We observed higher levels of anti-inflammatory IL-10, higher anti-inflammatory IL-6\/IL-10 ratios and lower levels of pro-inflammatory IL-12 and IL-18 as well as a trend of more anti-inflammatory IFNy in the 2000 \u00b5M NAD+ perfusate. In the bronchoalveolar lavage, the pro-inflammatory levels of IL-1\u03b1 and IL-1\u03b2 were lower in the 2000 \u00b5M NAD+ group. NAD+ administered during EVLP is a promising agent with both anti-inflammatory properties and the ability to improve ischemic lung function.<\/jats:p>","DOI":"10.3390\/antiox11050843","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T11:45:55Z","timestamp":1650973555000},"page":"843","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Ex Vivo Lung Perfusion with \u03b2-Nicotinamide Adenine Dinucleotide (NAD+) Improves Ischemic Lung Function"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0748-0436","authenticated-orcid":false,"given":"Jonas Peter","family":"Ehrsam","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery, University Hospital Zurich, 8091 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, University Hospital Zurich, 8091 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5855-8993","authenticated-orcid":false,"given":"Hector","family":"Rodriguez Cetina Biefer","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, City Hospital of Zurich, 8063 Zurich, Switzerland"},{"name":"Deutsches Herzzentrum Berlin, 13353 Berlin, Germany"},{"name":"Department of Cardiac Surgery, Charit\u00e9 Universit\u00e4tsmedizin Berlin, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5900-9040","authenticated-orcid":false,"given":"Isabelle","family":"Opitz","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, University Hospital Zurich, 8091 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5497-8256","authenticated-orcid":false,"given":"Stephan","family":"Arni","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, University Hospital Zurich, 8091 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3937-7705","authenticated-orcid":false,"given":"Ilhan","family":"Inci","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, University Hospital Zurich, 8091 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1016\/j.healun.2017.07.016","article-title":"The Registry of the International Society for Heart and Lung Transplantation: Thirty-fourth Adult Lung And Heart-Lung Transplantation Report-2017; Focus Theme: Allograft ischemic time","volume":"36","author":"Chambers","year":"2017","journal-title":"J. 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