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Despite their name, LPMOs possess a dominant peroxygenase activity that is reflected in high turnover numbers but also causes deactivation. We report on the influence of small molecules and ions on the activity and stability of LPMO during catalysis. Turbidimetric and photometric assays were used to identify LPMO inhibitors and measure their inhibitory effect. Selected inhibitors were employed to study LPMO activity and stability during cellulose depolymerization by HPLC and turbidimetry. It was found that the fungal metabolic products oxalic acid and citric acid strongly reduce LPMO activity, but also protect the enzyme from deactivation. QM calculations showed that the copper atom in the catalytic site could be ligated by bi- or tridentate chelating compounds, which replace two water molecules. MD simulations and QM calculations show that the most likely inhibition pattern is the competition between the inhibitor and reducing agent in the oxidized Cu(II) state. A correlation between the complexation energy and the IC50 values demonstrates that small, bidentate molecules interact strongest with the catalytic site copper and could be used by the fungus as physiological effectors to regulate LPMO activity.<\/jats:p>","DOI":"10.3390\/antiox11061096","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T09:24:29Z","timestamp":1653989069000},"page":"1096","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Inhibition of the Peroxygenase Lytic Polysaccharide Monooxygenase by Carboxylic Acids and Amino Acids"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4601-5895","authenticated-orcid":false,"given":"Erik","family":"Breslmayr","sequence":"first","affiliation":[{"name":"Institute of Food Technology, Department of Food Science and Technology, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria"},{"name":"Institute of Molecular Modeling and Simulation, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6272-4907","authenticated-orcid":false,"given":"Peter","family":"Poliak","sequence":"additional","affiliation":[{"name":"Institute of Molecular Modeling and Simulation, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria"},{"name":"Department of Chemical Physics, Faculty of Chemical and Food Technology, Slovak University of Technology, 812 37 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alen","family":"Po\u017egaj\u010di\u0107","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, Department of Food Science and Technology, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria"},{"name":"Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Schindler","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, Department of Food Science and Technology, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3856-3170","authenticated-orcid":false,"given":"Daniel","family":"Kracher","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, Department of Food Science and Technology, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria"},{"name":"Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4232-2556","authenticated-orcid":false,"given":"Chris","family":"Oostenbrink","sequence":"additional","affiliation":[{"name":"Institute of Molecular Modeling and Simulation, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5058-5874","authenticated-orcid":false,"given":"Roland","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, Department of Food Science and Technology, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15079","DOI":"10.1073\/pnas.1105776108","article-title":"Insights into the Oxidative Degradation of Cellulose by a Copper Metalloenzyme That Exploits Biomass Components","volume":"108","author":"Quinlan","year":"2011","journal-title":"Proc. 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