{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T09:29:07Z","timestamp":1778837347935,"version":"3.51.4"},"reference-count":38,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Italian Research Foundation for Amyotrophic Lateral Sclerosis\u2014AriSLA","award":["(ALSoDJ-1)"],"award-info":[{"award-number":["(ALSoDJ-1)"]}]},{"name":"the Italian Research Foundation for Amyotrophic Lateral Sclerosis\u2014AriSLA","award":["NIH P40OD018537"],"award-info":[{"award-number":["NIH P40OD018537"]}]},{"name":"Stocks were obtained from the Bloomington Drosophila Stock Center (BDSC)","award":["(ALSoDJ-1)"],"award-info":[{"award-number":["(ALSoDJ-1)"]}]},{"name":"Stocks were obtained from the Bloomington Drosophila Stock Center (BDSC)","award":["NIH P40OD018537"],"award-info":[{"award-number":["NIH P40OD018537"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Redox homeostasis is a vital process the maintenance of which is assured by the presence of numerous antioxidant small molecules and enzymes and the alteration of which is involved in many pathologies, including several neurodegenerative disorders. Among the different enzymes involved in the antioxidant response, SOD1 and DJ-1 have both been associated with the pathogenesis of amyotrophic lateral sclerosis and Parkinson\u2019s disease, suggesting a possible interplay in their mechanism of action. Copper deficiency in the SOD1-active site has been proposed as a central determinant in SOD1-related neurodegeneration. SOD1 maturation mainly relies on the presence of the protein copper chaperone for SOD1 (CCS), but a CCS-independent alternative pathway also exists and functions under anaerobic conditions. To explore the possible involvement of DJ-1 in such a pathway in vivo, we exposed Drosophila melanogaster to anoxia and evaluated the effect of DJ-1 on fly survival and SOD1 levels, in the presence or absence of CCS. Loss of DJ-1 negatively affects the fly response to the anoxic treatment, but our data indicate that the protective activity of DJ-1 is independent of SOD1 in Drosophila, indicating that the two proteins may act in different pathways.<\/jats:p>","DOI":"10.3390\/antiox11081527","type":"journal-article","created":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T21:03:50Z","timestamp":1659906230000},"page":"1527","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["DJ-1 and SOD1 Act Independently in the Protection against Anoxia in Drosophila melanogaster"],"prefix":"10.3390","volume":"11","author":[{"given":"Federica","family":"De Lazzari","sequence":"first","affiliation":[{"name":"Department of Biology, University of Padova, Via Ugo Bassi 58\/B, 35131 Padova, Italy"},{"name":"Medical Research Council, Mitochondria Biology Unit, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0XY, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9833-6498","authenticated-orcid":false,"given":"Francesco","family":"Agostini","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padova, Via Ugo Bassi 58\/B, 35131 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Doni","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padova, Via Ugo Bassi 58\/B, 35131 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4775-3857","authenticated-orcid":false,"given":"Sandro","family":"Malacrida","sequence":"additional","affiliation":[{"name":"Institute of Mountain Emergency Medicine, Eurac Research, 39100 Bolzano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro A.","family":"Zordan","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padova, Via Ugo Bassi 58\/B, 35131 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Costantini","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padova, Via Ugo Bassi 58\/B, 35131 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7927-9208","authenticated-orcid":false,"given":"Luigi","family":"Bubacco","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padova, Via Ugo Bassi 58\/B, 35131 Padova, Italy"},{"name":"Study Center for Neurodegeneration (CESNE), 35100 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5631-7467","authenticated-orcid":false,"given":"Federica","family":"Sandrelli","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padova, Via Ugo Bassi 58\/B, 35131 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3968-8418","authenticated-orcid":false,"given":"Marco","family":"Bisaglia","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padova, Via Ugo Bassi 58\/B, 35131 Padova, Italy"},{"name":"Study Center for Neurodegeneration (CESNE), 35100 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9215","DOI":"10.1002\/anie.202000451","article-title":"Superoxide Dismutase 1 in Health and Disease: How a Frontline Antioxidant Becomes Neurotoxic","volume":"60","author":"Trist","year":"2021","journal-title":"Angew. 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