{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:18:19Z","timestamp":1790651899728,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T00:00:00Z","timestamp":1660262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"School of Medicine Pilot Funding Award 2021 (Tulane University, New Orleans, LA, USA)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Seminal oxidative stress and sperm DNA damage are potential etiologies of male factor infertility. The present study aims to evaluate the relationship between oxidation-reduction potential (ORP), a measure of oxidative stress, and sperm DNA fragmentation (SDF) by conducting a systematic review and meta-analysis of relevant clinical data. A literature search was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The COVIDENCE tool was used to screen and identify studies evaluating seminal ORP and SDF. Studies (n = 7) that measured seminal ORP and SDF of 3491 semen samples were included in the analysis. The fixed-effects model revealed a significant pooled correlation coefficient (r = 0.24; p &lt; 0.001) between seminal ORP and SDF. Furthermore, subgroup analyses indicated that the pooled correlation coefficient between ORP and sperm chromatin dispersion (SCD) assay was less than other SDF assays (0.23 vs. 0.29). There was a moderate level of heterogeneity (I2 = 42.27%) among the studies, indicating a lack of publication bias. This is the first meta-analysis to reveal a positive correlation between seminal ORP and SDF. Furthermore, this study indicates the role of oxidative stress in the development of sperm DNA damage and thus warrants prospectively exploring the clinical value of these sperm function tests.<\/jats:p>","DOI":"10.3390\/antiox11081563","type":"journal-article","created":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T21:09:06Z","timestamp":1660511346000},"page":"1563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Association between Seminal Oxidation-Reduction Potential and Sperm DNA Fragmentation\u2014A Meta-Analysis"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9120-2278","authenticated-orcid":false,"given":"Manesh Kumar","family":"Panner Selvam","sequence":"first","affiliation":[{"name":"Department of Urology, School of Medicine, Tulane University, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4499-8680","authenticated-orcid":false,"given":"Saradha","family":"Baskaran","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine, Tulane University, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"O\u2019Connell","sequence":"additional","affiliation":[{"name":"Office of Academic Affairs and Provost, Tulane University, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wael","family":"Almajed","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine, Tulane University, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne J. G.","family":"Hellstrom","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine, Tulane University, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh C.","family":"Sikka","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine, Tulane University, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.clinbiochem.2018.03.012","article-title":"Fertility and infertility: Definition and epidemiology","volume":"62","author":"Wyns","year":"2018","journal-title":"Clin. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s00018-019-03253-8","article-title":"Oxidative stress and male infertility: Current knowledge of pathophysiology and role of antioxidant therapy in disease management","volume":"77","author":"Barati","year":"2020","journal-title":"Cell Mol. Life Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Aitken, R.J., Drevet, J.R., Moazamian, A., and Gharagozloo, P. (2022). Male Infertility and Oxidative Stress: A Focus on the Underlying Mechanisms. Antioxidants, 11.","DOI":"10.3390\/antiox11020306"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"296","DOI":"10.5534\/wjmh.190055","article-title":"Male Oxidative Stress Infertility (MOSI): Proposed Terminology and Clinical Practice Guidelines for Management of Idiopathic Male Infertility","volume":"37","author":"Agarwal","year":"2019","journal-title":"World J. Men\u2019s Health"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e13577","DOI":"10.1111\/and.13577","article-title":"Reactive oxygen species in male reproduction: A boon or a bane?","volume":"53","author":"Baskaran","year":"2021","journal-title":"Andrologia"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Aitken, R.J., and Drevet, J.R. (2020). The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword. Antioxidants, 9.","DOI":"10.3390\/antiox9020111"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"851","DOI":"10.2174\/0929867013373039","article-title":"Relative impact of oxidative stress on male reproductive function","volume":"8","author":"Sikka","year":"2001","journal-title":"Curr. Med. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.fertnstert.2016.05.013","article-title":"MiOXSYS: A novel method of measuring oxidation reduction potential in semen and seminal plasma","volume":"106","author":"Agarwal","year":"2016","journal-title":"Fertil. Steril."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.freeradbiomed.2020.05.008","article-title":"Scientific landscape of oxidative stress in male reproductive research: A scientometric study","volume":"156","author":"Agarwal","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e13938","DOI":"10.1111\/and.13938","article-title":"Redox potential in human semen: Validation and qualification of the MiOX(sys) assay","volume":"53","author":"Vassiliou","year":"2021","journal-title":"Andrologia"},{"key":"ref_11","first-page":"e13610","article-title":"Evaluation of seminal oxidation-reduction potential in male infertility","volume":"53","author":"Finelli","year":"2021","journal-title":"Andrologia"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1080\/14737159.2019.1614916","article-title":"Sperm DNA damage and its impact on male reproductive health: A critical review for clinicians, reproductive professionals and researchers","volume":"19","author":"Agarwal","year":"2019","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1093\/humrep\/deq214","article-title":"Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: Development of diagnostic criteria","volume":"25","author":"Aitken","year":"2010","journal-title":"Hum. Reprod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.rbmo.2014.02.004","article-title":"Sperm DNA damage caused by oxidative stress: Modifiable clinical, lifestyle and nutritional factors in male infertility","volume":"28","author":"Wright","year":"2014","journal-title":"Reprod. Biomed. Online"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s10815-018-1350-y","article-title":"Reactive oxygen species-induced alterations in H19-Igf2 methylation patterns, seminal plasma metabolites, and semen quality","volume":"36","author":"Darbandi","year":"2019","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 statement: An updated guideline for reporting systematic reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/j.0006-341X.2000.00455.x","article-title":"Trim and fill: A simple funnel-plot-based method of testing and adjusting for publication bias in meta-analysis","volume":"56","author":"Duval","year":"2000","journal-title":"Biometrics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e13258","DOI":"10.1111\/and.13258","article-title":"Correlation of oxidation-reduction potential with hormones, semen parameters and testicular volume","volume":"51","author":"Arafa","year":"2019","journal-title":"Andrologia"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e13609","DOI":"10.1111\/and.13609","article-title":"Seminal oxidation-reduction potential levels are not influenced by the presence of leucocytospermia","volume":"52","author":"Arafa","year":"2020","journal-title":"Andrologia"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Garcia-Segura, S., Ribas-Maynou, J., Lara-Cerrillo, S., Garcia-Peir\u00f3, A., Castel, A.B., Benet, J., and Oliver-Bonet, M. (2020). Relationship of Seminal Oxidation-Reduction Potential with Sperm DNA Integrity and pH in Idiopathic Infertile Patients. Biology, 9.","DOI":"10.3390\/biology9090262"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Gill, K., Kups, M., Harasny, P., Machalowski, T., Grabowska, M., Lukaszuk, M., Matuszewski, M., Duchnik, E., Fraczek, M., and Kurpisz, M. (2021). The Negative Impact of Varicocele on Basic Semen Parameters, Sperm Nuclear DNA Dispersion and Oxidation-Reduction Potential in Semen. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18115977"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Homa, S.T., Vassiliou, A.M., Stone, J., Killeen, A.P., Dawkins, A., Xie, J., Gould, F., and Ramsay, J.W. (2019). A Comparison Between Two Assays for Measuring Seminal Oxidative Stress and their Relationship with Sperm DNA Fragmentation and Semen Parameters. Genes, 10.","DOI":"10.3390\/genes10030236"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.aju.2017.11.014","article-title":"Oxidation-reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men","volume":"16","author":"Majzoub","year":"2018","journal-title":"Arab. J. Urol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1080\/14647273.2020.1712747","article-title":"Seminal oxidation-reduction potential and sperm DNA fragmentation index increase among infertile men with varicocele","volume":"25","author":"Tanaka","year":"2022","journal-title":"Hum. Fertil."},{"key":"ref_25","unstructured":"WHO (2021). WHO Laboratory Manual for the Examination and Processing of Human Semen, World Health Organization. [6th ed.]."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"31","DOI":"10.4103\/1008-682X.122203","article-title":"Oxidative stress and male reproductive health","volume":"16","author":"Aitken","year":"2014","journal-title":"Asian J. Androl."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/aja.2010.76","article-title":"Leukocytes and oxidative stress: Dilemma for sperm function and male fertility","volume":"13","author":"Henkel","year":"2011","journal-title":"Asian J. Androl."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1002\/j.1939-4640.1995.tb00566.x","article-title":"Role of oxidative stress and antioxidants in male infertility","volume":"16","author":"Sikka","year":"1995","journal-title":"J. Androl."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e13726","DOI":"10.1111\/and.13726","article-title":"An update on the techniques used to measure oxidative stress in seminal plasma","volume":"53","author":"Robert","year":"2021","journal-title":"Andrologia"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"565","DOI":"10.4103\/aja.aja_5_19","article-title":"Multi-center evaluation of oxidation-reduction potential by the MiOXSYS in males with abnormal semen","volume":"21","author":"Agarwal","year":"2019","journal-title":"Asian J. Androl."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"385","DOI":"10.4111\/icu.2017.58.6.385","article-title":"Oxidation-reduction potential as a new marker for oxidative stress: Correlation to male infertility","volume":"58","author":"Agarwal","year":"2017","journal-title":"Investig. Clin. Urol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"239","DOI":"10.5534\/wjmh.180014","article-title":"Abnormal Human Sperm Parameters Contribute to Sperm DNA Fragmentation in Men with Varicocele","volume":"36","author":"Park","year":"2018","journal-title":"World J. Men\u2019s Health"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1016\/j.fertnstert.2008.01.086","article-title":"Correlation between DNA damage and sperm parameters: A prospective study of 1633 patients","volume":"91","author":"Belloc","year":"2009","journal-title":"Fertil. Steril."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.urology.2017.02.016","article-title":"Clinical Relevance of Oxidation-Reduction Potential in the Evaluation of Male Infertility","volume":"104","author":"Agarwal","year":"2017","journal-title":"Urology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e12881","DOI":"10.1111\/and.12881","article-title":"Semen quality and infertility status can be identified through measures of oxidation-reduction potential","volume":"50","author":"Arafa","year":"2018","journal-title":"Andrologia"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e13874","DOI":"10.1111\/and.13874","article-title":"Sperm DNA fragmentation testing: Summary evidence and clinical practice recommendations","volume":"53","author":"Esteves","year":"2021","journal-title":"Andrologia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"935","DOI":"10.21037\/tau.2016.10.03","article-title":"Clinical utility of sperm DNA fragmentation testing: Practice recommendations based on clinical scenarios","volume":"5","author":"Agarwal","year":"2016","journal-title":"Transl. Androl. Urol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"412","DOI":"10.5534\/wjmh.200128","article-title":"Sperm DNA Fragmentation: A New Guideline for Clinicians","volume":"38","author":"Agarwal","year":"2020","journal-title":"World J. Men\u2019s Health"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1111\/andr.12743","article-title":"Predictive value of oxidative stress testing in semen for sperm DNA fragmentation assessed by sperm chromatin dispersion test","volume":"8","author":"Elbardisi","year":"2020","journal-title":"Andrology"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/11\/8\/1563\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:07:45Z","timestamp":1760141265000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/11\/8\/1563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,12]]},"references-count":39,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["antiox11081563"],"URL":"https:\/\/doi.org\/10.3390\/antiox11081563","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,12]]}}}