{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:06:13Z","timestamp":1788296773874,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T00:00:00Z","timestamp":1663200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deanship of Scientific Research, Qassim University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>This study evaluates the hepatoprotective activity of a Tamarix articulata extract against carbon tetrachloride-mediated hepatotoxicity in Wistar rats. Our results demonstrated that the oral administration of Tamarix articulata extract (50 mg\/kg b.w.) significantly restored the serum levels of liver enzymes and antioxidant parameters (superoxide dismutase, catalase, glutathione reductase, and thiobarbituric reactive substances). Histopathology analysis revealed that Tamarix articulata extract significantly reduced hepatic fibrosis by inhibiting the necrosis of hepatocytes. Furthermore, serum pro-inflammatory (tumor necrosis factor-alpha, tumor growth factor-beta, and interleukin-6) markers were significantly restored. However, the anti-inflammatory cytokine adiponectin levels increased to normal levels in the group treated with Tamarix articulata extract. Additionally, we observed diminished reactive oxygen species production and the depolarization of mitochondrial membrane potential in hepatocytes extracted from animal livers treated with Tamarix articulata extract. Our findings suggest that Tamarix articulata extract prevents liver fibrosis induced by carbon tetrachloride and decreases the necrotic population of hepatocytes. These events restored the antioxidant enzymatic activity, serum levels of liver enzymes, and pro-inflammatory markers to their normal levels.<\/jats:p>","DOI":"10.3390\/antiox11091824","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T22:11:10Z","timestamp":1663279870000},"page":"1824","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Tamarix articulata Induced Prevention of Hepatotoxicity Effects of In Vivo Carbon Tetrachloride by Modulating Pro-Inflammatory Serum and Antioxidant Enzymes to Reverse the Liver Fibrosis"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9306-609X","authenticated-orcid":false,"given":"Abdullah M.","family":"Alnuqaydan","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5709-4130","authenticated-orcid":false,"given":"Abdulmajeed G.","family":"Almutary","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9357-2216","authenticated-orcid":false,"given":"Mohammed","family":"A. Alsahli","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulaiman","family":"Alnasser","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Unaizah College of Pharmacy, Qassim University, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3673-5044","authenticated-orcid":false,"given":"Bilal","family":"Rah","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia"},{"name":"Iron Biology Group, Sharjah Institute of Medical Research, University of Sharjah, Sharjah 27272, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3024","DOI":"10.1111\/febs.15877","article-title":"Liver Macrophages and Inflammation in Physiology and Physiopathology of Non-alcoholic Fatty Liver Disease","volume":"289","author":"Thibaut","year":"2022","journal-title":"FEBS J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"88","DOI":"10.3109\/03602532.2011.602688","article-title":"Oxidant Stress, Mitochondria, and Cell Death Mechanisms in Drug-Induced Liver Injury: Lessons Learned from Acetaminophen Hepatotoxicity","volume":"44","author":"Jaeschke","year":"2012","journal-title":"Drug Metab. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8082","DOI":"10.3748\/wjg.v20.i25.8082","article-title":"Oxidative Stress as a Crucial Factor in Liver Diseases","volume":"20","author":"Michalak","year":"2014","journal-title":"World J. Gastroenterol."},{"key":"ref_4","first-page":"1065","article-title":"Polyphenols in Fruits and Vegetables and Its Effect on Human Health","volume":"5","author":"Lima","year":"2014","journal-title":"Food Nutr. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.ejmech.2012.12.062","article-title":"Recent Advances in Natural Products from Plants for Treatment of Liver Diseases","volume":"63","author":"Zhang","year":"2013","journal-title":"Eur. J. Med. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.jnutbio.2016.09.001","article-title":"Pomegranate Protects against Arsenic-Induced P53-Dependent ROS-Mediated Inflammation and Apoptosis in Liver Cells","volume":"38","author":"Choudhury","year":"2016","journal-title":"J. Nutr. Biochem."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Vun-Sang, S., Rodrigues, K.F., Dsouza, U.J., and Iqbal, M. (2022). Suppression of Oxidative Stress and Proinflammatory Cytokines Is a Potential Therapeutic Action of Ficus Lepicarpa B.(Moraceae) against Carbon Tetrachloride (CCl4)-Induced Hepatotoxicity in Rats. Molecules, 27.","DOI":"10.3390\/molecules27082593"},{"key":"ref_8","first-page":"7","article-title":"Pharmacological Evaluation of Allium Cepa Extract as Hepatoprotective Potential in Albino Rat","volume":"1","author":"Maurya","year":"2018","journal-title":"Int. J. Health Clin. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1515\/reveh-2020-0048","article-title":"Toxicity of Carbon Tetrachloride, Free Radicals and Role of Antioxidants","volume":"36","author":"Unsal","year":"2021","journal-title":"Rev. Environ. Health"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Karapetsas, A., Voulgaridou, G.-P., Konialis, M., Tsochantaridis, I., Kynigopoulos, S., Lambropoulou, M., Stavropoulou, M.-I., Stathopoulou, K., Aligiannis, N., and Bozidis, P. (2019). Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental in Vitro Skin Models. Antioxidants, 8.","DOI":"10.3390\/antiox8050125"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.ejmech.2019.06.010","article-title":"Medicinal Prospects of Antioxidants: A Review","volume":"178","author":"Neha","year":"2019","journal-title":"Eur. J. Med. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1007\/s00441-016-2445-3","article-title":"Reactive Oxygen Species and Fibrosis: Further Evidence of a Significant Liaison","volume":"365","author":"Richter","year":"2016","journal-title":"Cell Tissue Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.etap.2014.01.026","article-title":"Therapeutic Potential of Morin against Liver Fibrosis in Rats: Modulation of Oxidative Stress, Cytokine Production and Nuclear Factor Kappa B","volume":"37","author":"Heeba","year":"2014","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1684\/ecn.2013.0341","article-title":"Antioxidant, Anti-Inflammatory and Hepatoprotective Effects of Silymarin on Hepatic Dysfunction Induced by Sodium Nitrite","volume":"24","author":"Sherif","year":"2013","journal-title":"Eur. Cytokine Netw."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"131098","DOI":"10.1016\/j.foodchem.2021.131098","article-title":"Tea and Its Phytochemicals: Hidden Health Benefits & Modulation of Signaling Cascade by Phytochemicals","volume":"371","author":"Bag","year":"2022","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"285","DOI":"10.2174\/1389201020666190318120103","article-title":"Tamarix Articulata (T. Articulata)\u2014An Important Halophytic Medicinal Plant with Potential Pharmacological Properties","volume":"20","author":"Alnuqaydan","year":"2019","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3826","DOI":"10.5958\/0974-360X.2018.00701.1","article-title":"Phenolic Content, HPLC Analysis and Antioxidant Activity Extract from Tamarix Gallica and Tamarix Articulata Growing in Southeast of Algeria","volume":"11","author":"Said","year":"2018","journal-title":"Res. J. Pharm. Technol."},{"key":"ref_18","first-page":"089","article-title":"Evaluation of the Cytotoxic Activity of Tamarix Articulata and Its Anticancer Potential in Prostate Cancer Cells","volume":"12","author":"Rah","year":"2022","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2217","DOI":"10.1080\/14786419.2017.1366478","article-title":"Destabilizing the Interplay between MiR-1275 and IGF2BPs by Tamarix Articulata and Quercetin in Hepatocellular Carcinoma","volume":"32","author":"Shaalan","year":"2018","journal-title":"Nat. Prod. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2586","DOI":"10.1016\/j.sjbs.2020.05.028","article-title":"Comparative Assessment of Biological Activities of Different Parts of Halophytic Plant Tamarix Articulata (T. Articulata) Growing in Saudi Arabia","volume":"27","author":"Alnuqaydan","year":"2020","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"162","DOI":"10.17113\/ftb.59.02.21.6904","article-title":"Tamarix Articulata Inhibits Cell Proliferation, Promotes Cell Death Mechanisms and Triggers G0\/G1 Cell Cycle Arrest in Hepatocellular Carcinoma Cells","volume":"59","author":"Alnuqaydan","year":"2021","journal-title":"Food Technol. Biotechnol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"114920","DOI":"10.1016\/j.jep.2021.114920","article-title":"Cajanus Cajan Ameliorated CCl4-Induced Oxidative Stress in Wistar Rats via the Combined Mechanisms of Anti-Inflammation and Mitochondrial-Membrane Transition Pore Inhibition","volume":"289","author":"Nwaechefu","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"112953","DOI":"10.1016\/j.biopha.2022.112953","article-title":"Evaluation of the Hepatoprotective Activity of Hydroalcoholic Extract of Alhagi Camelorum against Valproic Acid-Induced Hepatotoxicity in Rats","volume":"150","author":"Iqbal","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1039\/C4FO00088A","article-title":"Hepatoprotective Effects of Secoiridoid-Rich Extracts from Gentiana Cruciata L. against Carbon Tetrachloride Induced Liver Damage in Rats","volume":"5","year":"2014","journal-title":"Food Funct."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ammar, N.M., Hassan, H.A., Abdallah, H.M., Afifi, S.M., Elgamal, A.M., Farrag, A.R.H., El-Gendy, A.E.-N.G., Farag, M.A., and Elshamy, A.I. (2022). Protective Effects of Naringenin from Citrus Sinensis (Var. Valencia) Peels against CCl4-Induced Hepatic and Renal Injuries in Rats Assessed by Metabolomics, Histological and Biochemical Analyses. Nutrients, 14.","DOI":"10.3390\/nu14040841"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1080\/01480545.2020.1792487","article-title":"Protection of CCl4-Induced Hepatic and Renal Damage by Linalool","volume":"45","author":"Mazani","year":"2022","journal-title":"Drug Chem. Toxicol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hamza, A.A., Lashin, F.M., Gamel, M., Hassanin, S.O., Abdalla, Y., and Amin, A. (2020). Hawthorn Herbal Preparation from Crataegus Oxyacantha Attenuates in Vivo Carbon Tetrachloride-Induced Hepatic Fibrosis via Modulating Oxidative Stress and Inflammation. Antioxidants, 9.","DOI":"10.3390\/antiox9121173"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Al-Megrin, W.A., Alkhuriji, A.F., Yousef, A.O.S., Metwally, D.M., Habotta, O.A., Kassab, R.B., Abdel Moneim, A.E., and El-Khadragy, M.F. (2019). Antagonistic Efficacy of Luteolin against Lead Acetate Exposure-Associated with Hepatotoxicity Is Mediated via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Activities. Antioxidants, 9.","DOI":"10.3390\/antiox9010010"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lin, S.-Y., Xu, D., Du, X.-X., Ran, C.-L., Xu, L., Ren, S.-J., Tang, Z.-T., Yin, L.-Z., He, C.-L., and Yuan, Z.-X. (2019). Protective Effects of Salidroside against Carbon Tetrachloride (CCl4)-Induced Liver Injury by Initiating Mitochondria to Resist Oxidative Stress in Mice. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20133187"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1182\/blood-2006-06-029934","article-title":"Solenopsin, the Alkaloidal Component of the Fire Ant (Solenopsis Invicta), Is a Naturally Occurring Inhibitor of Phosphatidylinositol-3-Kinase Signaling and Angiogenesis","volume":"109","author":"Arbiser","year":"2007","journal-title":"Blood"},{"key":"ref_31","first-page":"10444","article-title":"Ailanthone Exerts an Antitumor Function on the Development of Human Lung Cancer by Upregulating MicroRNA-195","volume":"120","author":"Hou","year":"2018","journal-title":"J. Cell. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"37416","DOI":"10.1074\/jbc.M605063200","article-title":"Mitochondrial Production of Reactive Oxygen Species Mediate Dicumarol-Induced Cytotoxicity in Cancer Cells","volume":"281","author":"Du","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_33","first-page":"1","article-title":"Dronabinol Has Preferential Antileukemic Activity in Acute Lymphoblastic and Myeloid Leukemia with Lymphoid Differentiation Patterns","volume":"16","author":"Salitzky","year":"2016","journal-title":"BMC Cancer"},{"key":"ref_34","first-page":"867","article-title":"Oxymatrine Induces Cell Cycle Arrest and Apoptosis and Suppresses the Invasion of Human Glioblastoma Cells through the EGFR\/PI3K\/Akt\/MTOR Signaling Pathway and STAT3","volume":"40","author":"Dai","year":"2018","journal-title":"Oncol. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Li, Y., Yao, J., Han, C., Yang, J., Chaudhry, M.T., Wang, S., Liu, H., and Yin, Y. (2016). Quercetin, Inflammation and Immunity. Nutrients, 8.","DOI":"10.3390\/nu8030167"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1002\/jcb.25407","article-title":"Resveratrol Increases Anti-proliferative Activity of Bestatin through Downregulating P-glycoprotein Expression via Inhibiting PI3K\/Akt\/MTOR Pathway in K562\/ADR Cells","volume":"117","author":"Wang","year":"2016","journal-title":"J. Cell. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Huang, T.-H., Lin, C.-F., Alalaiwe, A., Yang, S.-C., and Fang, J.-Y. (2019). Apoptotic or Antiproliferative Activity of Natural Products against Keratinocytes for the Treatment of Psoriasis. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20102558"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-8722-6-19","article-title":"Targeting Mitochondrial Reactive Oxygen Species as Novel Therapy for Inflammatory Diseases and Cancers","volume":"6","author":"Li","year":"2013","journal-title":"J. Hematol. Oncol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"451","DOI":"10.3109\/13880209.2015.1045086","article-title":"Protective Effect and Antioxidant Role of Sweetgum (Liquidambar Orientalis) Oil against Carbon Tetrachloride-Induced Hepatotoxicity and Oxidative Stress in Rats","volume":"54","author":"Suzek","year":"2016","journal-title":"Pharm. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Dutta, S., Chakraborty, A.K., Dey, P., Kar, P., Guha, P., Sen, S., Kumar, A., Sen, A., and Chaudhuri, T.K. (2018). Amelioration of CCl4 Induced Liver Injury in Swiss Albino Mice by Antioxidant Rich Leaf Extract of Croton Bonplandianus Baill. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0196411"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"29930","DOI":"10.1007\/s11356-019-06158-y","article-title":"Amelioration of Hepatic Function, Oxidative Stress, and Histopathologic Damages by Cassia Fistula L. Fraction in Thioacetamide-Induced Liver Toxicity","volume":"26","author":"Kaur","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Bischoff, K., Mukai, M., and Ramaiah, S.K. (2018). Liver Toxicity. Veterinary Toxicology, Elsevier.","DOI":"10.1016\/B978-0-12-811410-0.00015-5"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.cbi.2015.04.006","article-title":"Ameliorative Efficacy of Tetrahydrocurcumin against Arsenic Induced Oxidative Damage, Dyslipidemia and Hepatic Mitochondrial Toxicity in Rats","volume":"235","author":"Muthumani","year":"2015","journal-title":"Chem. Biol. Interact."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"26087","DOI":"10.3390\/ijms161125942","article-title":"The Role of Oxidative Stress and Antioxidants in Liver Diseases","volume":"16","author":"Li","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_45","unstructured":"Salam, A., and Farwin, S. (2017). Biochemistry of Reactive Oxygen Species in Selective Cancer Cell Toxicity and Protection of Normal Cells, University of Cincinnati."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e10209","DOI":"10.1002\/btm2.10209","article-title":"Mitochondrial Transplantation Therapy Inhibit Carbon Tetrachloride-induced Liver Injury through Scavenging Free Radicals and Protecting Hepatocytes","volume":"6","author":"Zhao","year":"2021","journal-title":"Bioeng. Transl. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.tox.2010.04.014","article-title":"Epigallocatechin-3-Gallate (EGCG) Reduces Liver Inflammation, Oxidative Stress and Fibrosis in Carbon Tetrachloride (CCl4)-Induced Liver Injury in Mice","volume":"273","author":"Tipoe","year":"2010","journal-title":"Toxicology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1080\/01635581.2018.1521442","article-title":"Effect of Phoenix Dactylifera L. Sap against Bleomycin-Induced Pulmonary Fibrosis and Oxidative Stress in Rats: Phytochemical and Therapeutic Assessment","volume":"71","author":"Bahri","year":"2019","journal-title":"Nutr. Cancer"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.mam.2018.06.003","article-title":"Organ and Tissue Fibrosis: Molecular Signals, Cellular Mechanisms and Translational Implications","volume":"65","author":"Weiskirchen","year":"2019","journal-title":"Mol. Asp. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.bbagen.2013.08.020","article-title":"Berberine and S Allyl Cysteine Mediated Amelioration of DEN+CCl4 Induced Hepatocarcinoma","volume":"1840","author":"Sengupta","year":"2014","journal-title":"Biochim. Biophys. 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