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Human and animal in vitro models have confirmed hypomineralization of differentiating cell lines with sidestream smoke being more harmful to developing cells than mainstream smoke. Furthermore, first reports are emerging to suggest a differential impact of conventional versus harm-reduction tobacco products on bone tissue as it develops in the embryo or in vitro. To gather first insight into the molecular mechanism of such differences, we assessed the effect of sidestream smoke solutions from Camel (conventional) and Camel Blue (harm-reduction) cigarettes using a human embryonic stem cell osteogenic differentiation model. Sidestream smoke from the conventional Camel cigarettes concentration-dependently inhibited in vitro calcification triggered by high levels of mitochondrially generated oxidative stress, loss of mitochondrial membrane potential, and reduced ATP production. Camel sidestream smoke also induced DNA damage and caspase 9-dependent apoptosis. Camel Blue-exposed cells, in contrast, invoked only intermediate levels of reactive oxygen species insufficient to activate caspase 3\/7. Despite the absence of apoptotic gene activation, damage to the mitochondrial phenotype was still noted concomitant with activation of an anti-inflammatory gene signature and inhibited mineralization. Collectively, the presented findings in differentiating pluripotent stem cells imply that embryos may exhibit low bone mineral density if exposed to environmental smoke during development.<\/jats:p>","DOI":"10.3390\/antiox11122474","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T02:16:41Z","timestamp":1671157001000},"page":"2474","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sidestream Smoke Extracts from Harm-Reduction and Conventional Camel Cigarettes Inhibit Osteogenic Differentiation via Oxidative Stress and Differential Activation of intrinsic Apoptotic Pathways"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6176-7895","authenticated-orcid":false,"given":"Nicole R. 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J. Public Health"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1177\/003335491312800308","article-title":"Estimates of smoking before and during pregnancy, and smoking cessation during pregnancy: Comparing two population-based data sources","volume":"128","author":"Tong","year":"2013","journal-title":"Public Health Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1007\/s00198-010-1415-y","article-title":"Parental smoking during pregnancy and offspring bone mass at age 10 years: Findings from a prospective birth cohort","volume":"22","author":"Tobias","year":"2011","journal-title":"Osteoporos. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1359\/jbmr.2001.16.9.1694","article-title":"Neonatal bone mass: Influence of parental birthweight, maternal smoking, body composition, and activity during pregnancy","volume":"16","author":"Godfrey","year":"2001","journal-title":"J Bone Miner Res."},{"key":"ref_5","unstructured":"Centers for Disease Control and Prevention (CDC) (2008). Smoking prevalence among women of reproductive age\u2014United States, 2006. MMWR Morb. Mortal. Wkly. Rep., 57, 849\u2013852."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.bone.2017.05.007","article-title":"Maternal smoking during pregnancy is associated with childhood bone fractures in offspring\u2014A birth-cohort study of 6718 children","volume":"101","author":"Parviainen","year":"2017","journal-title":"Bone"},{"key":"ref_7","unstructured":"Burns, D.M., and Benowitz, N.L. (2001). Public health implications of changes in cigarette design and marketing, National Cancer Institute. Risks Associated with Smoking Cigarettes with Low Machine-Measured Yields of Tar and Nicotine, Smoking and Tobacco Control Monograph No. 13; NIH Publication No. 02-5074."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/stem.2746","article-title":"Low osteogenic yield in human pluripotent stem cells associates with differential neural crest promoter methylation","volume":"36","author":"Sparks","year":"2018","journal-title":"Stem Cells"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1093\/ntr\/ntac144","article-title":"Exposure to cigarette smoke impedes human in vitro osteoblast differentiation independently of nicotine","volume":"24","author":"Martinez","year":"2022","journal-title":"Nicotine Tob. Res."},{"key":"ref_10","unstructured":"U.S. Department of Health and Human Services (2006). The Health Consequences of Involuntary Exposure to Tobacco Smoke: A Report of the Surgeon General. Secondhand Smoke What It Means to You."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Rasmussen, T.P. (2018). Risk Assessment Using Human Pluripotent Stem Cells: Recent Advances in Developmental Toxicity Screens. Stem Cells in Birth Defects Research and Developmental Toxicology, Wiley.","DOI":"10.1002\/9781119283249"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Karmach, O., Madrid, J.V., Dasgupta, S., Volz, D.C., and zur Nieden, N.I. (2022). Embryonic Exposure to Cigarette Smoke Extract Impedes Skeletal Development and Evokes Craniofacial Defects in Zebrafish. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23179904"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/bdrc.20085","article-title":"Oxidative stress as a mechanism of teratogenesis","volume":"78","author":"Hansen","year":"2006","journal-title":"Birth Defects Res. C Embryo Today"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1002\/bdrc.20098","article-title":"Effects of oxidative stress on embryonic development","volume":"81","author":"Dennery","year":"2007","journal-title":"Birth Defects Res. C Embryo Today"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1002\/bdrc.20081","article-title":"Mechanism of teratogenesis: Electron transfer, reactive oxygen species, and antioxidants","volume":"78","author":"Kovacic","year":"2006","journal-title":"Birth Defects Res. C Embryo Today"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1164\/rccm.200512-1835OC","article-title":"Clusterin decreases oxidative stress in lung fibroblasts exposed to cigarette smoke","volume":"174","author":"Carnevali","year":"2006","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_17","unstructured":"Sies, H. (1985). Oxidative Stress, Academic Press."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0021-9150(03)00053-4","article-title":"High oxidative stress in patients with stable coronary heart disease","volume":"168","author":"Weinbrenner","year":"2003","journal-title":"Atherosclerosis"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.atherosclerosis.2004.08.007","article-title":"Association between novel oxidative stress markers and C-reactive protein among adults without clinical coronary heart disease","volume":"178","author":"Abramson","year":"2005","journal-title":"Atherosclerosis"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1089\/ars.2006.1465","article-title":"Involvement of oxidative stress in the pathogenesis of diabetes","volume":"9","author":"Kaneto","year":"2007","journal-title":"Antioxid. Redox. Signal."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.mrgentox.2008.09.017","article-title":"Oxidative stress in environmental-induced carcinogenesis","volume":"674","author":"Mena","year":"2009","journal-title":"Mutat. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1095\/biolreprod53.1.29","article-title":"Ciliary beat frequency of hamster oviducts is decreased in vitro by exposure to solutions of mainstream and sidestream cigarette smoke","volume":"53","author":"Knoll","year":"1995","journal-title":"Biol. Reprod."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0890-6238(97)00100-7","article-title":"Cigarette smoke inhibits oocyte cumulus complex pick-up by the oviduct in vitro independent of ciliary beat frequency","volume":"12","author":"Knoll","year":"1998","journal-title":"Reprod. Toxicol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/978-1-60761-962-8_17","article-title":"Absorption-based assays for the analysis of osteogenic and chondrogenic yield","volume":"690","author":"Davis","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1002\/ar.22641","article-title":"Quantitative analysis of histological staining and fluorescence using ImageJ","volume":"296","author":"Jensen","year":"2013","journal-title":"Anat. Rec."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.acthis.2017.03.001","article-title":"A simple ImageJ macro tool for analyzing mitochondrial network morphology in mammalian cell culture","volume":"119","author":"Valente","year":"2017","journal-title":"Acta Histochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_28","unstructured":"Oliveros, J.C. (2022, June 06). (2007\u20132015) Venny. An Interactive Tool for Comparing Lists with Venn\u2019s Diagrams. Available online: https:\/\/bioinfogp.cnb.csic.es\/tools\/venny\/index.h."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.diff.2015.11.002","article-title":"Cardiac differentiation potential of human induced pluripotent stem cells in a 3D self-assembling peptide scaffold","volume":"90","author":"Semino","year":"2015","journal-title":"Differentiation"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.taap.2003.09.019","article-title":"Molecular multiple endpoint embryonic stem cell test--a possible approach to test for the teratogenic potential of compounds","volume":"194","author":"Kempka","year":"2004","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.taap.2010.05.010","article-title":"Comparing three novel endpoints for developmental osteotoxicity in the embryonic stem cell test","volume":"247","author":"Davis","year":"2010","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1038\/nprot.2011.348","article-title":"The validated embryonic stem cell test to predict embryotoxicity in vitro","volume":"6","author":"Seiler","year":"2011","journal-title":"Nat. Protoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/978-1-4939-7883-0_5","article-title":"Human Pluripotent Stem Cells to Assess Developmental Toxicity in the Osteogenic Lineage","volume":"1797","author":"Madrid","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.toxrep.2014.11.016","article-title":"Non-human primate and rodent embryonic stem cells are differentially sensitive to embryotoxic compounds","volume":"2","author":"Walker","year":"2014","journal-title":"Toxicol. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Walker, L.M., Sparks, N.R.L., Puig-Sanvicens, V., Rodrigues, B., and zur Nieden, N.I. (2021). An Evaluation of Human Induced Pluripotent Stem Cells to Test for Cardiac Developmental Toxicity. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22158114"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"11147","DOI":"10.1074\/jbc.M413787200","article-title":"c-Abl tyrosine kinase regulates caspase-9 autocleavage in the apoptotic response to DNA damage","volume":"280","author":"Raina","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6006","DOI":"10.1167\/iovs.09-3628","article-title":"Dual involvement of caspase-4 in inflammatory and ER stress-induced apoptotic responses in human retinal pigment epithelial cells","volume":"50","author":"Bian","year":"2009","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"35631","DOI":"10.1074\/jbc.M005401200","article-title":"c-Abl has high intrinsic tyrosine kinase activity that is stimulated by mutation of the Src homology 3 domain and by autophosphorylation at two distinct regulatory tyrosines","volume":"275","author":"Brasher","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0092-8674(02)00623-2","article-title":"Autoinhibition of c-Abl","volume":"108","author":"Pluk","year":"2002","journal-title":"Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4523","DOI":"10.1038\/sj.onc.1202722","article-title":"Bcl-2\/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2\/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability","volume":"18","author":"Imazu","year":"1999","journal-title":"Oncogene"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1096\/fj.04-3329fje","article-title":"Oxidative Bax dimerization promotes its translocation to mitochondria independently of apoptosis","volume":"19","author":"Coppola","year":"2005","journal-title":"FASEB J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1042\/BJ20070319","article-title":"Bnip3 mediates mitochondrial dysfunction and cell death through Bax and Bak","volume":"405","author":"Kubli","year":"2007","journal-title":"Biochem. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"11247","DOI":"10.1074\/jbc.M809392200","article-title":"Cleavage of Bid by executioner caspases mediates feed forward amplification of mitochondrial outer membrane permeabilization during genotoxic stress-induced apoptosis in Jurkat cells","volume":"284","author":"Shelton","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_44","first-page":"a008714","article-title":"Mechanisms of Action of Bcl-2 Family Proteins","volume":"5","author":"Kale","year":"2013","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_45","first-page":"1206235","article-title":"The Effect of Tobacco Smoking on Bone Mass: An Overview of Pathophysiologic Mechanisms","volume":"2018","author":"Haddad","year":"2018","journal-title":"J. Osteoporos."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1006\/taap.1999.8789","article-title":"Normal patterns of angiogenesis and extracellular matrix deposition in chick chorioallantoic membranes are disrupted by mainstream and sidestream cigarette smoke","volume":"163","author":"Melkonian","year":"2000","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1093\/toxsci\/68.1.237","article-title":"Mainstream and sidestream cigarette smoke inhibit growth and angiogenesis in the day 5 chick chorioallantoic membrane","volume":"68","author":"Melkonian","year":"2002","journal-title":"Toxicol. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1179\/135100001101536274","article-title":"A comparative study by electron paramagnetic resonance of free radical species in the mainstream and sidestream smoke of cigarettes with conventional acetate filters and \u2018bio-filters\u2019","volume":"6","author":"Valavanidis","year":"2001","journal-title":"Redox. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1095\/biolreprod.105.041152","article-title":"Cigarette smoke inhibits hamster oocyte pickup by increasing adhesion between the oocyte cumulus complex and oviductal cilia","volume":"73","author":"Gieseke","year":"2005","journal-title":"Biol. Reprod."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1093\/humrep\/dei080","article-title":"Sidestream smoking is equally as damaging as mainstream smoking on IVF outcomes","volume":"20","author":"Neal","year":"2005","journal-title":"Hum. Reprod."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1093\/humrep\/den419","article-title":"Comparison of toxicity of smoke from traditional and harm-reduction cigarettes using mouse embryonic stem cells as a novel model for preimplantation development","volume":"24","author":"Lin","year":"2009","journal-title":"Hum. Reprod."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.taap.2009.03.019","article-title":"Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse","volume":"237","author":"Polyzos","year":"2009","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1093\/humrep\/del380","article-title":"Smoke from traditional commercial, harm-reduction, and research brand cigarettes impairs oviductal functioning in hamsters (Merocricetus auratus) in vitro","volume":"22","author":"Riveles","year":"2007","journal-title":"Human Reprod."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1080\/009841097160393","article-title":"The changing cigarette, 1950\u20131995","volume":"50","author":"Hoffmann","year":"1997","journal-title":"J. Toxicol. Environ. Health"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1002\/tera.1420400505","article-title":"Susceptible period of nitrous oxide teratogenicity in Sprague-Dawley rats","volume":"40","author":"Fujinaga","year":"1989","journal-title":"Teratology"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1159\/000146751","article-title":"Maternal and fetal chromosomal aberrations in mice following prenatal exposure to subembryotoxic doses of lead nitrate","volume":"135","author":"Nayak","year":"1995","journal-title":"Acta Anat."},{"key":"ref_57","first-page":"719","article-title":"Adverse birth outcomes associated with maternal smoking and polymorphisms in the N-Nitrosamine-metabolizing enzyme genes NQO1 and CYP2E1","volume":"67","author":"Sasaki","year":"2008","journal-title":"Am. J. Epidemiol."},{"key":"ref_58","first-page":"8","article-title":"New questions and insights into nitrate\/nitrite and human health effects: A retrospective cohort study of private well users\u2019 immunological and wellness status","volume":"74","author":"Zeman","year":"2011","journal-title":"J. Environ. Health"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.reprotox.2012.09.004","article-title":"Redox control of teratogenesis","volume":"35","author":"Hansen","year":"2013","journal-title":"Reprod. Toxicol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2286","DOI":"10.1096\/fj.01-0206fje","article-title":"The mitochondrial permeability transition initiates autophagy in rat hepatocytes","volume":"15","author":"Elmore","year":"2001","journal-title":"FASEB J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1161\/CIRCRESAHA.111.255216","article-title":"Redox signaling in cardiac physiology and pathology","volume":"111","author":"Burgoyne","year":"2012","journal-title":"Circ. Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3389\/fcell.2016.00063","article-title":"Redox Events as Modulators of Pathology and Therapy of Neuroinflammatory Diseases","volume":"4","author":"Lepka","year":"2016","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1242\/dev.128.1.137","article-title":"DNA damage-induced neural precursor cell apoptosis requires p53 and caspase 9 but neither Bax nor caspase 3","volume":"128","author":"Leonard","year":"2001","journal-title":"Development"},{"key":"ref_64","first-page":"5815","article-title":"Apoptosis induced by DNA damage O6-methylguanine is Bcl-2 and caspase-9\/3 regulated and Fas\/caspase-8 independent","volume":"60","author":"Ochs","year":"2000","journal-title":"Cancer Res."},{"key":"ref_65","first-page":"217037","article-title":"Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions","volume":"2012","author":"Sharma","year":"2012","journal-title":"Am. J. Bot."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"33888","DOI":"10.1074\/jbc.M702969200","article-title":"Caspase-9-induced mitochondrial disruption through cleavage of anti-apoptotic BCL-2 family members","volume":"282","author":"Chen","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1111\/j.1365-3083.2009.02331.x","article-title":"Feedback regulation of mitochondria by caspase-9 in the B cell receptor-mediated apoptosis","volume":"70","author":"Eeva","year":"2009","journal-title":"Scand. J. Immunol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1093\/cvr\/cvn104","article-title":"Mitochondrial fission mediates high glucose-induced cell death through elevated production of reactive oxygen species","volume":"79","author":"Yu","year":"2008","journal-title":"Cardiovasc. Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1007\/s00125-010-1770-4","article-title":"Mitochondrial fragmentation and superoxide anion production in coronary endothelial cells from a mouse model of type 1 diabetes","volume":"53","author":"Makino","year":"2010","journal-title":"Diabetologia"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/s00125-009-1553-y","article-title":"Diabetes regulates mitochondrial biogenesis and fission in mouse neurons","volume":"53","author":"Edwards","year":"2010","journal-title":"Diabetologia"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.exer.2014.04.003","article-title":"All-trans-retinal induces Bax activation via DNA damage to mediate retinal cell apoptosis","volume":"123","author":"Sawada","year":"2014","journal-title":"Exp. Eye Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1038\/sj.cdd.4400783","article-title":"Pro-apoptotic cascade activates BID, which oligomerizes BAK or BAX into pores that result in the release of cytochrome c","volume":"7","author":"Korsmeyer","year":"2000","journal-title":"Cell Death Differ."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"402","DOI":"10.15252\/embj.201592789","article-title":"Bax assembles into large ring-like structures remodeling the mitochondrial outer membrane in apoptosis","volume":"35","author":"Wurm","year":"2016","journal-title":"EMBO J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"11549","DOI":"10.1074\/jbc.274.17.11549","article-title":"An APAF-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9","volume":"274","author":"Zou","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1038\/sj.emboj.7601533","article-title":"Bak and Bax are non-redundant during infection- and DNA damage-induced apoptosis","volume":"26","author":"Keep","year":"2007","journal-title":"EMBO J."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.bbabio.2013.10.015","article-title":"The endoplasmic reticulum-mitochondria connection: One touch, multiple functions","volume":"1837","author":"Marchi","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Cagalinec, M., Liiv, M., Hodurova, Z., Hickey, M.A., Vaarmann, A., Mandel, M., Zeb, A., Choubey, V., Kuum, M., and Safiulina, D. (2016). Role of Mitochondrial Dynamics in Neuronal Development: Mechanism for Wolfram Syndrome. PLoS Biol., 14.","DOI":"10.1371\/journal.pbio.1002511"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1093\/jmcb\/mjw004","article-title":"Mitochondria-associate endoplasmic reticulum membranes allow adaptation of mitochondrial metabolism to glucose availability in the liver","volume":"8","author":"Theurey","year":"2016","journal-title":"J. Mol. Cell Biol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ceca.2017.01.003","article-title":"The endoplasmic reticulum-mitochondria coupling in health and disease: Molecules, functions and significance","volume":"62","author":"Filadi","year":"2017","journal-title":"Cell Calcium"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"eaaq1380","DOI":"10.1126\/scisignal.aaq1380","article-title":"ER-mitochondria cross-talk is regulated by the Ca2+ sensor NCS1 and is impaired in Wolfram syndrome","volume":"11","author":"Angebault","year":"2018","journal-title":"Sci. Signal."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1083\/jcb.200310015","article-title":"Involvement of caspase-4 in endoplasmic reticulum stress-induced apoptosis and Abeta-induced cell death","volume":"165","author":"Hitomi","year":"2004","journal-title":"J. Cell Biol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1254\/jphs.10217SC","article-title":"Caspase-4 Directly Activates Caspase-9 in Endoplasmic Reticulum Stress-Induced Apoptosis in SH-SY5Y Cells","volume":"115","author":"Yamamuro","year":"2011","journal-title":"J. Pharmacol. Sci."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"17908","DOI":"10.1074\/jbc.M113.458836","article-title":"Transmembrane Protein 214 (TMEM214) mediates endoplasmic reticulum stress-induced caspase 4 enzyme activation and apoptosis","volume":"288","author":"Li","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/B978-0-12-385114-7.00004-0","article-title":"Measuring ER stress and the unfolded protein response using mammalian tissue culture system","volume":"490","author":"Oslowski","year":"2011","journal-title":"Methods Enzymol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.neuint.2007.08.017","article-title":"Presenilin-1 mutation activates the signaling pathway of caspase-4 in endoplasmic reticulum stress-induced apoptosis","volume":"52","author":"Yukioka","year":"2008","journal-title":"Neurochem. Int."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/sj.onc.1201192","article-title":"Involvement of caspase-4(-like) protease in Fas-mediated apoptotic pathway","volume":"15","author":"Kamada","year":"1997","journal-title":"Oncogene"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1089\/oli.2007.0064","article-title":"Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4","volume":"17","author":"Lin","year":"2007","journal-title":"Oligonucleotides"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"33078","DOI":"10.1074\/jbc.M607904200","article-title":"The marine product cephalostatin 1 activates an endoplasmic reticulum stress-specific and apoptosome-independent apoptotic signaling pathway","volume":"281","author":"Rudy","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1002\/eji.201545523","article-title":"Caspase-4 mediates non-canonical activation of the NLRP3 inflammasome in human myeloid cells","volume":"45","author":"Gaidt","year":"2015","journal-title":"Eur. J. Immunol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"8480","DOI":"10.4049\/jimmunol.179.12.8480","article-title":"Caspase-4 interacts with TNF receptor-associated factor 6 and mediates lipopolysaccharide-induced NF-kappaB-dependent production of IL-8 and CC chemokine ligand 4 (macrophage-inflammatory protein-1)","volume":"179","author":"Lakshmanan","year":"2007","journal-title":"J. Immunol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"26041","DOI":"10.1074\/jbc.M602222200","article-title":"NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages","volume":"281","author":"Cao","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"14978","DOI":"10.1073\/pnas.0809784106","article-title":"Tolerance and M2 (alternative) macrophage polarization are related processes orchestrated by p50 nuclear factor kappaB","volume":"106","author":"Porta","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1053\/j.gastro.2004.06.013","article-title":"Interleukin 10-deficient mice develop osteopenia, decreased bone formation, and mechanical fragility of long bones","volume":"127","author":"Gelb","year":"2004","journal-title":"Gastroenterology"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"4917","DOI":"10.1096\/fj.201701256RRR","article-title":"Concentration-dependent, dual roles of IL-10 in the osteogenesis of human BMSCs via P38\/MAPK and NF-kappaB signaling pathways","volume":"32","author":"Chen","year":"2018","journal-title":"FASEB J."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"2991","DOI":"10.1002\/1521-4141(200010)30:10<2991::AID-IMMU2991>3.0.CO;2-1","article-title":"Induction of the IL-10 gene via the fas receptor in monocytes--an anti-inflammatory mechanism in the absence of apoptosis","volume":"30","author":"Daigle","year":"2000","journal-title":"Eur. J. Immunol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"G820","DOI":"10.1152\/ajpgi.00438.2005","article-title":"IL-10 protects mouse intestinal epithelial cells from Fas-induced apoptosis via modulating Fas expression and altering caspase-8 and FLIP expression","volume":"291","author":"Bharhani","year":"2006","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"784","DOI":"10.3389\/fimmu.2018.00784","article-title":"Role of TNF-TNF Receptor 2 Signal in Regulatory T Cells and Its Therapeutic Implications","volume":"9","author":"Yang","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1359\/jbmr.1997.12.10.1637","article-title":"Fas and Fas ligand interaction is necessary for human osteoblast apoptosis","volume":"12","author":"Kawakami","year":"1997","journal-title":"J. Bone Miner Res."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1007\/s002230010069","article-title":"The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses","volume":"66","author":"Hatakeyama","year":"2000","journal-title":"Calcif. Tissue Int."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.4049\/jimmunol.170.3.1540","article-title":"Increased bone mass is a part of the generalized lymphoproliferative disorder phenotype in the mouse","volume":"170","author":"Lorenzo","year":"2003","journal-title":"J. Immunol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.lfs.2003.06.031","article-title":"Non-functional Fas ligand increases the formation of cartilage early in the endochondral bone induction by rhBMP-2","volume":"74","author":"Vucenik","year":"2003","journal-title":"Life Sci."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/11\/12\/2474\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:42:13Z","timestamp":1760146933000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/11\/12\/2474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,15]]},"references-count":101,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["antiox11122474"],"URL":"https:\/\/doi.org\/10.3390\/antiox11122474","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,15]]}}}