{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T13:58:37Z","timestamp":1793282317691,"version":"4.5.0"},"reference-count":56,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,31]],"date-time":"2022-12-31T00:00:00Z","timestamp":1672444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>An acidic polysaccharide fraction was obtained from Calocybe indica (CIP3a) after subjecting it to hot water extraction followed by purification through DEAE-cellulose 52 and Sepaharose 6B column chromatography. The CIP3a was further modified using chloroacetic acid to yield carboxymethylated derivatives (CMCIP3a). The modified polysaccharide was characterized using various spectroscopic methods. In addition, further antioxidant, antitumor and anticoagulant activities were also investigated. The polysaccharides CIP3a and CMCIP3a were heterogeneous in nature and composed of various molar percentages of glucose, arabinose and mannose with molecular weights of 1.456 \u00d7 103 and 4.023 \u00d7 103 Da, respectively. The NMR and FT-IR data demonstrated that the carboxymethylation on the polysaccharide was successful. In comparison to CIP3a polysaccharides, the modified derivatives had lower sugar and protein contents, and higher levels of uronic acid. The in vitro antioxidant activity showed that CMCIP3a with higher molecular weight displayed an elevated ability in scavenging the DPPH radical, ABTS, superoxide, hydroxyl radical, ferric reducing power, cupric reducing power and erythrocyte hemolysis inhibition with an EC50 value of 2.49, 2.66, 4.10, 1.60, 3.48, 1.41 and 2.30 mg\/mL, respectively. The MTT assay results revealed that CMCIP3a displayed a dose-dependent inhibition on five cancer cells (HT29, PC3, HeLa, Jurkat and HepG-2) in the range of 10\u2013320 \u03bcg\/mL. The APTT, PT and TT were significantly extended by CMCIP3a in relation to dosage, indicating that the anticoagulant effect of CIP was both extrinsic and intrinsic, along with a common coagulation pathway. These findings demonstrated that carboxymethylation might effectively improve the biological potential of the derivatives and offer a theoretical framework for the creation of novel natural antioxidants, low-toxicity antitumor and antithrombotic drugs.<\/jats:p>","DOI":"10.3390\/antiox12010105","type":"journal-article","created":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T02:12:48Z","timestamp":1672625568000},"page":"105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Effects of Carboxymethyl Modification on the Acidic Polysaccharides from Calocybe indica: Physicochemical Properties, Antioxidant, Antitumor and Anticoagulant Activities"],"prefix":"10.3390","volume":"12","author":[{"given":"Ambika","family":"Nataraj","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Biosciences, Periyar University, Salem 636001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7416-5591","authenticated-orcid":false,"given":"Sudha","family":"Govindan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Biosciences, Periyar University, Salem 636001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Rajendran","sequence":"additional","affiliation":[{"name":"Dhanvanthri Laboratory, Department of Sciences, Amrita School of Physical Sciences, Amrita Vishwa Vidyapeetham, Coimbatore 641112, India"},{"name":"Center of Excellence in Advanced Materials & Green Technologies (CoE\u2013AMGT), Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore 641112, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5236-7141","authenticated-orcid":false,"given":"Prasanna","family":"Ramani","sequence":"additional","affiliation":[{"name":"Dhanvanthri Laboratory, Department of Sciences, Amrita School of Physical Sciences, Amrita Vishwa Vidyapeetham, Coimbatore 641112, India"},{"name":"Center of Excellence in Advanced Materials & Green Technologies (CoE\u2013AMGT), Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore 641112, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnamoorthy Akkana","family":"Subbaiah","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo E. S.","family":"Munekata","sequence":"additional","affiliation":[{"name":"Centro Tecnol\u00f3gico de la Carne de Galicia, Avd. Galicia No. 4, Parque Tecnol\u00f3gico de Galicia, 32900 San Cibrao das Vi\u00f1as, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9682-4594","authenticated-orcid":false,"given":"Mirian","family":"Pateiro","sequence":"additional","affiliation":[{"name":"Centro Tecnol\u00f3gico de la Carne de Galicia, Avd. Galicia No. 4, Parque Tecnol\u00f3gico de Galicia, 32900 San Cibrao das Vi\u00f1as, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7725-9294","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"Lorenzo","sequence":"additional","affiliation":[{"name":"Centro Tecnol\u00f3gico de la Carne de Galicia, Avd. 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Agric."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"10155","DOI":"10.1016\/j.fbio.2022.101955","article-title":"Bioactive polysaccharides from medicinal mushrooms: A review on their isolation, structural characteristics and antitumor activity","volume":"49","author":"Garcia","year":"2022","journal-title":"Food Biosci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.tifs.2021.03.008","article-title":"Advances in dietary polysaccharides as anticancer agents: Structure-activity relationship","volume":"111","author":"Li","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jfutfo.2022.09.003","article-title":"Medicinal value of edible mushroom polysaccharides: A review","volume":"3","author":"Yu","year":"2023","journal-title":"J. Future Foods"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.carbpol.2018.11.012","article-title":"Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques","volume":"206","author":"Huang","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.ijbiomac.2022.01.192","article-title":"Effects of sulfation and carboxymethylation on Cy-clocarya paliurus polysaccharides: Physicochemical properties, antitumor activities and protection against cel-lular oxidative stress","volume":"204","author":"Xie","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1016\/j.ijbiomac.2019.11.078","article-title":"Preparation, characteristics, and an-tioxidant activities of carboxymethylated polysaccharides from blackcurrant fruits","volume":"155","author":"Duan","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"113079","DOI":"10.1016\/j.indcrop.2020.113079","article-title":"Extraction, structure characterization, carboxymethylation and antioxidant activity of acidic polysaccharides from Craterellus cornucopioides","volume":"159","author":"Liu","year":"2021","journal-title":"Ind. Crops Prod."},{"key":"ref_9","first-page":"e14121","article-title":"Structure characterization and antioxidant activity of carboxymethylated polysaccharide from Pholiota nameko","volume":"46","author":"Zhang","year":"2022","journal-title":"J. Food Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.carbpol.2009.03.028","article-title":"Carboxymethylation of a polysaccharide extracted from Ganoderma lucidum enhances its antioxidant activities in vitro","volume":"78","author":"Xu","year":"2009","journal-title":"Carbohydr. Polym."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.ijbiomac.2014.08.045","article-title":"Carboxymethylation of polysaccharides from Tremella fuciformis for antioxidant and moisture-preserving activities","volume":"72","author":"Wang","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.procbio.2018.06.002","article-title":"Structure characterization, modification through carboxymethylation and sulfation, and in vitro antioxidant and hypoglycemic activities of a polysaccharide from Lachnum sp","volume":"72","author":"Wang","year":"2018","journal-title":"Process Biochem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"114319","DOI":"10.1016\/j.indcrop.2021.114319","article-title":"Isolation, structural modification, characterization, and bioactivity of polysaccharides from Folium isatidis","volume":"176","author":"Abuduwaili","year":"2022","journal-title":"Ind. Crops Prod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"108555","DOI":"10.1016\/j.carres.2022.108555","article-title":"Carboxymethylation modification, characterization, antioxidant activity and anti-UVC ability of Sargassum fusiforme polysaccharide","volume":"515","author":"An","year":"2022","journal-title":"Carbohydr Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.ijbiomac.2019.01.029","article-title":"Purification, antitumor and anti-inflammation activities of an alkali-soluble and carboxymethyl polysaccharide CMP33 from Poria cocos","volume":"127","author":"Liu","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.ijbiomac.2009.04.010","article-title":"Chemical modification and antitumor activities of two polysaccharide-protein complexes from Pleurotus tuber-regium","volume":"45","author":"Tao","year":"2009","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.fshw.2021.04.015","article-title":"Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats","volume":"10","author":"Govindan","year":"2021","journal-title":"Food Sci. Hum. Wellness."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e05964","DOI":"10.1016\/j.heliyon.2021.e05964","article-title":"Sulfated modification, characterization and bioactivities of an acidic polysaccharide fraction from an edible mushroom Pleurotus eous (Berk.) Sacc","volume":"7","author":"Gunasekaran","year":"2021","journal-title":"Heliyon"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100308","DOI":"10.1016\/j.bcdf.2022.100308","article-title":"Novel studies of characterization, antioxidant, anticoagulant and anticancer activity of purified polysaccharide from Hypsizygus ulmarius mushroom","volume":"27","author":"Thimmaraju","year":"2022","journal-title":"Bioact. Carbohydr. Diet. Fibre."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Nataraj, A., Govindan, S., Ramani, P., Subbaiah, K.A., Sathianarayanan, S., Venkidasamy, B., Thiruvengadam, M., Rebezov, M., Shariati, M.A., and Lorenzo, J.M. (2022). Antioxidant, anti-tumour, and anticoagulant activities of polysaccharide from Calocybe indica (APK2). Antioxidants, 11.","DOI":"10.3390\/antiox11091694"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.etp.2016.04.001","article-title":"Antioxidant and anti-aging activities of polysaccharides from Calocybe indica var. APK2","volume":"68","author":"Govindan","year":"2016","journal-title":"Exp. Toxicol. Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/(SICI)1097-0126(199801)45:1<27::AID-PI900>3.0.CO;2-9","article-title":"Characterization of anacardium occidentale exudate polysaccharide","volume":"45","author":"Heatley","year":"1998","journal-title":"Polym. Int."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/j.ijbiomac.2019.09.211","article-title":"Effects of sulfated, phosphorylated and carboxymethylated modifications on the antioxidant activities in-vitro of polysaccharides sequentially extracted from Amana edulis","volume":"146","author":"Cao","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.ijbiomac.2017.02.065","article-title":"Preparation, characterization, and \u03b1-glycosidase inhibition activity of a carboxymethylated polysaccharide from the residue of Sarcandra glabra (Thunb.) Nakai","volume":"99","author":"Liu","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101209","DOI":"10.1016\/j.fbio.2021.101209","article-title":"Investigation of chemical and biological properties of an acidic polysaccharide fraction from Pleurotus eous (Berk.) Sacc","volume":"42","author":"Gunasekaran","year":"2021","journal-title":"Food Biosci."},{"key":"ref_26","first-page":"152","article-title":"Detection of antioxidant activity and bioactive constituents in the fruiting bodies of Hericium erinaceus pers-an edible mushroom","volume":"8","author":"Charumathy","year":"2016","journal-title":"Int. J. Pharm. Pharm. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.indcrop.2017.10.064","article-title":"Antioxidant and anticoagulant activities of mycelia polysaccharides from Catathelasma ventricosum after sulfated modification","volume":"112","author":"Liu","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.carbpol.2006.10.001","article-title":"Carboxymethylation of Tamarind kernel powder","volume":"69","author":"Goyal","year":"2007","journal-title":"Carbohydr. Polym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1016\/j.carbpol.2015.10.017","article-title":"Carboxymethylation of polysaccharide from Cyclocarya paliurus and their characterization and antioxidant properties evaluation","volume":"136","author":"Wang","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.carbpol.2008.02.019","article-title":"Structure characterization and carboxymethylation of arabinoxylan isolated from Ispaghula (Plantago ovata) seed husk","volume":"74","author":"Saghir","year":"2008","journal-title":"Carbohydr. Polym."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1111\/ijfs.13382","article-title":"Carboxymethylation of corn bran polysaccharide and its bioactive property","volume":"52","author":"Li","year":"2017","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.ijbiomac.2020.11.118","article-title":"A novel serine protease with anticoagulant and fibrinolytic activities from the fruiting bodies of mushroom Agrocybe aegerita","volume":"168","author":"Li","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.carbpol.2017.06.050","article-title":"The characterization, selenylation and antidiabetic activity of mycelial polysaccharides from Catathelasma ventricosum","volume":"174","author":"Liu","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.carbpol.2017.12.083","article-title":"Structural characterization, antioxidant and hepatoprotective activities of polysaccharides from Sophorae tonkinensis Radix","volume":"184","author":"Cai","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.carbpol.2015.07.031","article-title":"Preparation, characterization and antioxidant activities of acetylated polysaccharides from Cyclocarya paliurus leaves","volume":"133","author":"Xie","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3368","DOI":"10.1021\/jf063003p","article-title":"Structural and biological study of carboxymethylated Phellinus linteus polysaccharides","volume":"55","author":"Shin","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.indcrop.2018.12.047","article-title":"Structural features, antioxidant and acetylcholinesterase inhibitory activities of polysaccharides from stem of Physalis alkekengi L.","volume":"129","author":"Liu","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1039\/C8FO02190B","article-title":"Comparative study on the physicochemical properties and bioactivities of polysaccharide fractions extracted from Fructus mori at different temperatures","volume":"10","author":"Zhang","year":"2019","journal-title":"Food Funct."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.ijbiomac.2019.09.232","article-title":"The antioxidant activities of carboxymethylated garlic polysaccharide and its derivatives","volume":"145","author":"Cheng","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.ijbiomac.2013.08.023","article-title":"In vitro antioxidant and antitumor activities of different sulfated polysaccharides isolated from three algae","volume":"62","author":"Shao","year":"2013","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0003-2697(87)90222-3","article-title":"The deoxyribose method: A simple \u201ctest-tube\u201d assay for determination of rate constants for reactions of hydroxyl radicals","volume":"165","author":"Halliwell","year":"1987","journal-title":"Anal. Biochem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1016\/j.ijbiomac.2011.09.011","article-title":"Carboxymethylation of polysaccharides from Auricularia auricula and their antioxidant activities in vitro","volume":"49","author":"Yang","year":"2011","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1016\/j.ijbiomac.2018.09.052","article-title":"Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica","volume":"120","author":"Hammami","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1016\/j.carbpol.2014.10.046","article-title":"Preparation, characterization and bioactivities of derivatives of an exopolysaccharide from Lachnum","volume":"117","author":"He","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1016\/j.foodchem.2007.08.073","article-title":"Antioxidant properties of polysaccharides from Ganoderma tsugae","volume":"107","author":"Tseng","year":"2008","journal-title":"Food Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.foodhyd.2015.02.018","article-title":"Sulfated modification, characterization and antioxidant activities of polysaccharide from Cyclocarya paliurus","volume":"53","author":"Xie","year":"2016","journal-title":"Food Hydrocoll."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1016\/j.jfda.2016.10.007","article-title":"Antihemolytic and antioxidant properties of pearl powder against 2,2\u2032-azobis (2- amidinopropane) dihydrochloride-induced hemolysis and oxidative damage to erythrocyte membrane lipids and proteins","volume":"25","author":"Yang","year":"2017","journal-title":"J. Food Drug Anal."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/0891-5849(92)90102-M","article-title":"Kinetics and site specificity of hydroperoxide-induced oxidative damage in red blood cells","volume":"12","author":"Lubin","year":"1992","journal-title":"Free Radic. Biol. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.intimp.2010.01.002","article-title":"Immunopotentiation and anti-tumor activity of carboxymethylated-sulfated \u03b2-(1\u21923)-d-glucan from Poria cocos","volume":"10","author":"Chen","year":"2010","journal-title":"Int. Immunopharmacol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"10565","DOI":"10.1021\/jf902597w","article-title":"Enhancement of antitumor activities in sulfated and carboxymethylated polysaccharides of Ganoderma lucidum","volume":"57","author":"Wang","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Liu, H., Li, F., and Luo, P. (2019). Effect of carboxymethylation and phosphorylation on the properties of polysaccharides from Sepia esculenta Ink: Antioxidation and anticoagulation in vitro. Mar Drugs, 17.","DOI":"10.3390\/md17110626"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.intimp.2014.06.027","article-title":"Carboxymethylation enhances the maturation-inducing activity in dendritic cells of polysaccharide from the seeds of Plantago asiatica L.","volume":"22","author":"Jiang","year":"2014","journal-title":"Int. Immunopharmacol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.foodchem.2014.01.111","article-title":"Acetylation and carboxymethylation of the polysaccharide from Ganoderma atrum and their antioxidant and immunomodulating activities","volume":"156","author":"Chen","year":"2014","journal-title":"Food Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.carbpol.2015.01.062","article-title":"Carboxylate groups play a major role in antitumor activity of Ganoderma applanatum polysaccharide","volume":"123","author":"Sun","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.ijbiomac.2013.03.031","article-title":"Antioxidant activity and physicochemical properties of an acidic polysaccharide from Morinda officinalis","volume":"58","author":"Zhang","year":"2013","journal-title":"Int. J. Biol. Macromol."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/12\/1\/105\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:49:06Z","timestamp":1760147346000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/12\/1\/105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,31]]},"references-count":56,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["antiox12010105"],"URL":"https:\/\/doi.org\/10.3390\/antiox12010105","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,31]]}}}